In artificial joints, adsorbed protein films from synovial fluid significantly influence tribological behavior. While film structure
(lamellar for low friction, heterogeneous for high friction) and frictional properties are known, their formation process has been
unclear. This study investigates normal load history effects on the formation of adsorbed bovine serum albumin (BSA) films on
CoCrMo-UHMWPE interfaces and their frictional characteristics. Friction tests using a reciprocating tribometer with
in-situ
electrochemical analysis monitored both the coefficient of friction (COF) and net protein adsorption/desorption behavior. Load
was varied incrementally, from low to high (L→H) or high to low (H→L). Results showed distinct, history-dependent behaviors.
The L→H case exhibited an exponential COF increase, correlated with a shift from protein desorption to adsorption. Conversely,
the H→L case demonstrated a stable low COF, where an initial adsorption event indicated film stabilization. This study proposes a
mechanism where initiating friction at high loads denatures BSA, forming a uniform and low-friction film. Initiating at low loads
forms BSA aggregates, creating a heterogeneous and high-friction film. These findings clarify that load history is a critical
parameter governing protein film formation, providing a mechanical basis for previously observed phenomena.
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