Viva Origino
Online ISSN : 1346-6933
Print ISSN : 0910-4003
Volume 33, Issue 3
Displaying 1-11 of 11 articles from this issue
  • Haruichi Asahara, Jun Iwaki, Junji Yokozawa, Koji Tamura, Nobukazu Nam ...
    2005 Volume 33 Issue 3 Pages 194-207
    Published: 2005
    Released on J-STAGE: January 29, 2022
    JOURNAL FREE ACCESS

    Identity determinants of Escherichia coli tRNATyr were examined using in vitro transcripts. The first and second anticodon nucleotides were base-specifically involved in tRNA recognition of tyrosyl-tRNA synthetase, whereas the third anticodon nucleotide was not. None of the identity determinants were found in the acceptor stem, except the discriminator base A73. With respect to the long variable arm, a stem of three base pairs in length was required for tyrosylation, while the sequence of the arm was not essential. From the results of identity conversion experiments, the discrimination modes of tRNATyr from other amino acid specific tRNAs sharing some of the identity determinants of tRNATyr were discussed.

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