Rapid methods for the microbiological assay of dissolved (free) vitamin B_<12> and biotin in seawater have been developed by using Lactobacillus delbrueckii subsp. lactis ATCC 7830 and Lactobacillus plantarum ATCC 8014 as assay organisms, respectively. Desalting and concentration of B_<12> (or biotin) in seawater were achieved by using Sep-pak C_<18> (or Amberlite XAD-2 column). Recoveries of B_<12> and biotin from seawater were 92% and 89%, respectively. The level of biotin in seawater determined by the method using L. plantarum was compared with that determined by the current method using Achromobacter sp. yH-51.
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