A method of evaluating sulfonylurea herbicide (SU) resistance using surviving weeds after herbicide application in a paddy field was developed. Samples of
Scirpus juncoides Roxb. subsp.
junsoides Roxb. (height, 5-20cm),
Monochoria vaginalis Presl var.
plantaginea Solms-Laub. (3-7-leaf stage) and
Lindernia dubia Penn. (height, 5-10cm) were thoroughly rinsed in water. The shoots and roots were cut 2cm above and below the base of the shoots, respectively. The cuttings were transplanted into pots filled with paddy soil. Bensulfuron-methyl at 7.5g a. i./10a was applied 24 hours after transplanting. As the results, SU-susceptible biotype weeds exhibited little or no shoot regeneration, but SU-resistant biotype weeds exhibited vigorous shoot regeneration. The extent of regrowth of shoots was significantly different between SU-susceptible biotype and SU-resistant biotype weeds. Therefore, this evaluation method, named “shoot-regeneration method” can be used practically and efficiently in identifying SU-resistant biotype weeds in paddy fields.
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