YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
113 巻, 2 号
選択された号の論文の7件中1~7を表示しています
  • 大熊 誠一
    1993 年 113 巻 2 号 p. 95-113
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    The purification and serological and chemical properties of Vicia graminea lectin (VGA) and Vicia unijuga lectin (VUA) were described, and then the binding-specificity of anti-M and -N antibodies and both the lectins was discussed in this review. On the basis of the facts that Vgu glycoproteins which react with either VGA or VUA but not with anti-M and -N antibodies and were not detected in human normal organ tissues and sera, were separated and identified as mucin-type glycoproteins with very high molecular weights from human cancerous organ tissues, ascitic fluids of cancer patients and cyst fluids of human ovarian cystadenoma in malignant, it was concluded that Vgu glycoproteins are new tumor-associated substances.
  • 中嶋 暉躬
    1993 年 113 巻 2 号 p. 114-132
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    Biologically active materials were characterized chemically from various kinds of amphibian skin, and from the venom of wasps or spiders. The assay system employed for the biological activity on smooth muscle, were vaso-constriction and-delation, smooth muscle contraction and relaxation. Cytotrophic action including haemolysis, granulocyte degranulation, platelet aggregation, chemotaxis of leucocyte, and modulation of neural synaptic transmission were adopted for screening of the other materials. Many kinds of active peptide honologous to mammaliam peptide hormone, for example thyrotropin-releasing hormone (TRH), neurotensin, cholecystokinin (CCK), angiotensin, were found in high concentration from the methanol extract of the frog skin. A new cytotrophic peptide family was found both in frog skin and wasp venom. These peptides were rich in hydrophobic amino acids and basic ones. A peptide named mastoparan acts directly on the GTP binding protein as the hormone mimetic action and degranulates histamine granules of the mast cells. Another peptide revealing chemotactic activity for neutrophils acts directly on the FMLP receptor of the cells. These types of cytotrophic peptides modulate a cell signalling system via GTP-binding protein in such cells. Finally, over 15 kinds of neurotoxin were found from the spider venom which inhibit synaptic transmission of glutamate, both for lobster neuromuscular junction and mammaliam hippocampal neurons. A specific binding protein for this type of spider toxin was isolated from the bovin brain. The protein is a family of calreticulin previously isolated as a calcium regulating protein from sarcoplasmic reticulum in skeletal muscle.
  • 宮原 正信, 大高 博, 片山 博, 巽 義男, 宮一 論起範, 富森 毅
    1993 年 113 巻 2 号 p. 133-154
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    145 flavonoids were studied for their inhibitory effects on the ironinduced lipid peroxidation in mitochondria obtained from rat livers. Of these compounds tested, 30, 57, 59, 67, 70, 72, 77, 102 and 110 (ED50≤0.5nmol/mg prot) showed distinctly more potent inhibitory activity than baicalein (ED50≤5nmol/mg prot) and 59 and 72 (ED50≤0.05nmol/mg prot) exhibited the most potent activity. In order to elucidate the relationships between substituents on the flavonoid skeleton and the biological activity, the quantitative structure-activity relationships (QSAR) were analyzed by the adaptive least-squares (ALS) method for 142 flavonoids. The analysis has shown that the presence of the 1, 4- and 1, 2-hydroquinon in A-ring and/or B-ring of flavonoids and hydrophobicity of the molecule are responsible for the in vitro inhibitory activity.
  • 上田 條二, 門馬 則子, 大澤 啓助
    1993 年 113 巻 2 号 p. 155-158
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    A simple method using ion-pair high-performance liquid chromatography was established for the rapid and precise simultaneous determination of honokiol (3', 5-di-2-propenyl-1, 1'-biphenyl-2, 4'-diol) and magnolol (5, 5'-di-2-propenyl-1, 1'-biphenyl-2, 2'-diol) in oriental pharmaceutical decoctions containing Magnolia bark. An ODS column and a mixture of water involving 10 mM tetra-n-amylammonium bromide (TAA) and acetonitorile (4 : 6) as a mobile phase were used for the separation. Honokiol and magnolol were eluted without interference of other co-existing components within 12 min.
  • 渡辺 厚, 大沼 惟子
    1993 年 113 巻 2 号 p. 159-166
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    Crystal morphology, habit and refractive indices necessary for the analyses of amobarbital, barbital, phase III and ephedrine hydrochloride were studied using a polarizing microscope and partly using a reflexing goniometer. These substances, market products of Japanese Pharmacopoeia XII, were measured for optical properties including key refractive indices to confirm their modifications, and their possible facial angles along the zone of which axis is parallel to the elongated direction was also measured using a goniometer. Stereographic projections were drawn and the crystal habits of market products were shown by orthographic projections. It was also found that one of the key refractive indices of each modification was specially usable for the analyses of crystal habits including their crushed powders.
  • 黒野 幸久, 宮島 誠, 池田 憲
    1993 年 113 巻 2 号 p. 167-175
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    The interactions of camptothecin (CPT) and its derivatives (CPT-11 and SN-38) with human plasma proteins (serum albumin (HSA) and α1-acid glycoprotein (α1-AGP)) were studied mainly by means of ultraviolet and fluorescence spectroscopy. The binding constants of lactone ring-opened forms (A form) of CPT and CPT-11 with HSA were larger than those of the intact lactone forms (L form). In the case of SN-38, there was no difference in the constants between A form and L form. The binding constant of CPT (A form) with HSA was larger than those of CPT-11 and SN-38. The presence of cisplatin, which is presumed to be coadministered with CPT derivatives, did not affect the interaction of CPT derivatives with HSA. Only L form of CPT-11 among the CPT derivatives examined interacted with α1-AGP, followed by quenching the fluorescence of α1-AGP.
  • 岡本 光美, 塚原 民夫
    1993 年 113 巻 2 号 p. 176-179
    発行日: 1993/02/25
    公開日: 2008/05/30
    ジャーナル フリー
    Proscillaridin in commercial tablet preparations was analyzed by HPLC on a phenylpropyl chemically bonded stationary phase, prepared by treating porous silica with phenylpropyldimethylchlorosilane (PHP). From the elemental analysis data for carbon, the number of bonded PHP surface groups per 1 nm2 of silica (mean pore diameter 13.1 nm, specific surface area 330 m2/g, mean particle size 5.5 μm) in PHP gel was calculated to be 2.07. Using methanol-distilled water (80 : 20, v/v) as an eluent, the separation of proscillaridin was successfully performed on the PHP column.
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