巻号一覧
124 巻, 5 号
選択された号の論文の5件中1～5を表示しています
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総説
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二井 將光, 孫(和田) 戈虹, 和田 洋2004 年 124 巻 5 号 p. 243-260
発行日: 2004/05/01
公開日: 2004/04/29
ジャーナル フリーProton-translocating ATPases are essential cellular energy converters that transduce the chemical energy of ATP hydrolysis into transmembrane proton electrochemical potential differences. The structures, catalytic mechanism, and cellular functions of three major classes of ATPases including the F-type, V-type, and P-type ATPase are discussed in this review. Physiological roles of the acidic organelles and compartments contained are also discussed.
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田渕 圭章2004 年 124 巻 5 号 p. 261-268
発行日: 2004/05/01
公開日: 2004/04/29
ジャーナル フリーThe use of in vitro cell culture models has been of central importance in the development of our understanding of the cellular and molecular biology of organs and tissues. Transgenic mice and rats harboring temperature-sensitive simian virus 40 large T-antigen genes are useful for establishing cell lines from organs and tissues that have proved difficult to culture in vitro. Many conditionally immortalized cell lines with normal specific functions were generated from transgenic animals. DNA microarray technology has broad applications and is directed toward the study of global gene expression. Using established cell lines and DNA microarrays, we identified many genes that were up- and down-regulated in the process of the cell differentiation or cell death. In this review, the characteristics of established cell lines and possible applications of the study of global gene expression are discussed.
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大石 真也2004 年 124 巻 5 号 p. 269-277
発行日: 2004年
公開日: 2004/04/29
ジャーナル フリーA structure-activity relationship study was performed on cyclic RGD peptides using a combination of multisubstituted alkene dipeptide isosteres. To clarify the effects on bioactivity of a valine N-methyl group in the cyclo(-Arg-Gly-Asp-D-Phe-MeVal-) peptide developed by Kessler's group, novel D-Phe-Val-type isosteres with methyl-substituting groups on the olefin were designed and synthesized. Syntheses of D-Phe-ψ[(E)-CH=CMe]-Val-type isosteres were carried out in essentially identical fashion to the previously reported preparation of ψ[(E)-CH=CH]-type congeners. Alternatively, D-Phe-ψ[(E)-CMe=CX]-Val-type isosteres (X=H or Me) were synthesized via stereoselective alkylation of β-(1,3-oxazolidin-2-on-5-yl)-α,β-enoates using organocopper reagents. The resulting four isosteres were utilized in either solution- or solid-phase peptide synthesis to afford the cyclic RGD peptidomimetics, cyclo(-Arg-Gly-Asp-D-Phe-ψ[(E)-CX=CX]-Val-) (X=H or Me). αVβ3 and αIIbβ3 integrin antagonistic activities of the peptidomimetics along with Kessler's peptides were comparatively evaluated. In addition, structural calculations of these compounds by simulated annealing/energy minimization using dihedral and distance restraints derived from 1H-NMR data in DMSO gave insight into the effects of the valine N-methyl group as well as the D-phenylalanine carbonyl oxygen.
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一般論文
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宮崎 智雄, 関根 祐子, 青山 隆夫, 安野 伸浩, 中村 均, 山田 安彦, 伊賀 立二2004 年 124 巻 5 号 p. 279-286
発行日: 2004/05/01
公開日: 2004/04/29
ジャーナル フリーQuestionnaires were sent out to the staffs (13 physicians, 52 nurses and 5 medical engineers) of the ICU/CCU at the University of Tokyo Hospital, to evaluate pharmaceutical services by analyzing problems in the services offered. Four components of pharmaceutical services were evaluated: inventory control of drugs, check of drug usage and doses, mixing of injections, and offering drug information. Almost all responses from medical staffs evaluated pharmaceutical services overall as “good”. The high response rate (96%) from the nursing staff was attributed to the fact that they were familiar with the pharmacist's role with drug inventory, and mixing injections, when nursing was not available for these tasks. Although 50% of physicians rated the pharmaceutical services of providing drug information as “good”, this value was lower than responses on other items of the questionnaires, which suggests some dissatisfaction. The occurrences of drug information obtained by passive offering (121 subjects) was 4 times as common as drug information obtained by active offering (30 subjects). From this finding, and comments on the questionnaires from physicians, it suggests that physicians require more drug information for dosage regimens, and prefer the drug information to be provided more actively. Further, an important comment from physicians and nurses was that the services of pharmacists are not available on all shifts/all days of the week to provide consultation for drug information and mixing of injections. Although having a pharmacist available daily around the clock is desirable and ideal to the medical team, the number of pharmacists under the present system cannot support this. As a solution, we think that it is crucial that pharmacists educate medical staff when they are present to in order to optimize therapy and patient care over time.
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資料
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加藤 久美, 見崎 知子, 山崎 祥子, 新田 都子, 長谷川 美鶴, 神谷 悦功, 細田 蓮子2004 年 124 巻 5 号 p. 287-291
発行日: 2004/05/01
公開日: 2004/04/29
ジャーナル フリーIn Japan, trasdermal fentanyl (Durotep® Patch) was launched in March 2002, and it was regarded as making opioid rotation possible. When changing from morphine to transdermal fentanyl, the efficacy ratio of 1:150 is used in Japan as well as in many other countries. However, the ratio of 1:100 is used in Germany. As a result, a dose increase in transdermal fentanyl is often required to control pain. We studied transdermal fentanyl use in the Aichi Cancer Center (ACC) to investigate the actual conversion ratio and appropriate switching by following up 144 patients (81 men, 63 women) who had received transdermal fentanyl in the ACC from March 19, 2002, to April 30, 2003. Transdermal fentanyl improved pain control in patients who had difficulty in tolerating oral medication or in continuing morphine because of side effects. Regression analysis indicated that the efficacy ratio of oral morphine to transdermal fentanyl was 1:78. As the fentanyl dosage was excessive even in some patients who followed the recommended morphine/fentanyl conversion of 150:1, it is dangerous to use the conversion ratio of 78:1 at first. Morphine side effects were reduced in some patients who changed to transdermal fentanyl, but there was no reduction in those who needed high-dose morphine for rescue analgesia. Therefore it is safe and effective to use low-dose transdermal fentanyl in the beginning and to control pain promptly using rescue morphine based on the present recommended dosage. For opioid rotation, quick-acting opioids other than morphine are expected to be launched in Japan.
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