Although Chargaff has not made any investigation regarding separation of bonded lipoid in diphtherial bacteria, the authors saponified this lipoid and obtained reddish-brown pigment of carotene series, m.p. 184°, from non-saponifiable substance. From the saponified portion were obtained lauric acid, m.p. 44°, myristic acid, m.p. 54°, palmitic acid, m.p. 63°, stearic acid, m.p. 68°, and cerotic acid, m.p. 78°, which were all identified by mixed fusion with authentic compounds. As unsaturated acids, palmito-oleic acid was obtained which was identified by comparison with standard compound as dihydroxy-palmito-oleic acid, m.p. 130°. Dihydroxyacetone, m.p. 695° (dibenzoate, m.p. 125°) was obtained from the aqueous solution which was also identified by mixed fusion. From the aqueous solution was also obtained yellow crystals, m.p. 140°, which was assumed to be phenylhydrazone of hexose, and colorless crystals, m p. 110° (semicarbazone, decompn. 168°), C
6H
13NO
5, which coincides with d-glucosamine. Evaporation of aqueous solution by conc. hydrochloric acidity, isolation by Jones-Foremarr method gave two portions, one being C
6H
13NO
2, m.p. 293-295°, and the other, C
5H
9NO
4, m.p. 202°, which were identified as leucine and glutamine, respectively.
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