The calibration curves of the main compounds among 2, 2′-biquinoline derivatives (unsubstituted, 4, 4′-dimethyl, 4, 4′, 6, 6′- and 4, 4′, 7, 7′-tetramethyl, 4, 4′-diphenyl, 4, 4′-diphenyl-6, 6′- and -7, 7′-dimethyl, 4, 4′-dimethyl-6, 6′-dimethoxy, 1, 1′-dimethyl-3, 3′-bibenzo[
f]quinoline), in isopentyl alcohol and dimethylformamide, were established.
The extraction method with isopentyl alcohol was found to be very suitable for the micro quantitative determination of copper (ca. less than 1γ/cc.) and, 4, 4′-diphenyl-6, 6′-dimethyl-2, 2′-biquinoline (PMB), the most sensitive reagent among them, has been employed for the determination. UV spectrum of PMB, as well as UV and visible region spectra of cuprous chelate were measured and the colorimetric determination of trace of copper has been established by measuring at 554mμ. The molar ratio of copper to PMB was found to be 1:2 in isopentyl alcohol and it may be capable to determine quantitatively within 0.02γ to 0.8γ/cc., following the Beer's law. Various conditions of pH, temperature, as well as the copresent ions in this determination was examined and the quantitative determination of copper in animal organs was attempted.
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