Ultraviolet light irradiation (UV irradiation) was applied to p-nitrophenyl β-D-gluco-pyranosiduronic acid (PNG) in various systems ; (i) acetate buffer pH 4.0, (ii) acetate buffer-CuSO
4, (iii) acetate buffer-FeSO
4, (iv) acetate buffer-hydrogen peroxide, (v) acetate buffer-CuSO
4-hydrogen peroxide, (vi) acetate buffer-FeSO
4-hydrogen peroxide. In all the systems investigated, the cleavage of PNG was clearly observed, however, the ratio of this cleavage was different in each system. The cleavage was : a few per cent in i, 30 per cent in iv, 39 per cent in v, and 70 per cent in vi, respectively. From the above results it was assumed that UV irradiation might have a promoting effect on the formation of hydroxyl radical·OH, and thus the hydroxyl radical formed would have a potent influence on the cleavage of PNG.
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