In order to examine the course of intravenously injected iron chondroitinsulfate phagocytized in the reticuloendothelial system and the iron in it utilized as hemoglobin iron, iron chondroitinsulfate labeled with
59Fe was injected intravenously in normal mice, in three different doses, and examinations were made on its systemic distribution, separation of
59Fe into hemin iron and non-hemin iron in liver and spleen, and periodical changes in non-hemin
59Fe in the fraction separated by the Yoneyama -Konno method from the liver and small intestine, and the following points were revealed. 1) Disappearance of
59Fe from blood immediately after intravenous injection of iron (
59Fe) chondroitinsulfate, its incorporation into the liver, followed by its disappearance from the liver, and incorporation of
59Fe into erythrocytes all become rapid with decreased amount administered. 2). Introduction of
59Fe into hemin iron is not observed in the liver but the peak of
59Fe in the spleen during 8-96 hours after the intravenous injection is clearly due to the incorporation of
59Fe into hemin iron. 3) Formation of ferritin in the liver requires a certain time lag and there is a good correspondence between that and incorporation of
59Fe into erythrocytes. 4)
59Fe that has collected in the small intestine is partly excreted and its excretion rate is dependend on the dose administered but follows the first-order formula.
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