YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
Volume 98 , Issue 9
Showing 1-26 articles out of 26 articles from the selected issue
  • MASAYASU KIMURA, ISAMI WAKI, TADAHARU HONKE, MATAO KANAOKA
    1978 Volume 98 Issue 9 Pages 1139-1144
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    The fraction of smooth muscle-contracting substances (SMC) extracted from dry ox bile was examined using isolated guinea pig ileum. The dialyzable SMC fraction was soluble in water and ethanol, and reacted with both ninhydrin and Dragendorff's reagents. The contracting activity in products of the SMC fraction hydrolyzed by crude pseudocholinesterase or hydrochloric acid decreased to less than 1/10 or to less than 1/100, respectively, though did not decrease by either α-chymotrypsin or trypsin. Active choline esters and ninhydrin-positive substances were obtained from the SMC fraction, using ion-exchange column chromatography on Dowex 50W-X2.
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  • ITSUO MORI, YOSHIKAZU FUJITA, TAKEHISA ENOKI
    1978 Volume 98 Issue 9 Pages 1145-1151
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    The behavior of o-hydroxyhydroquinonephthalein (Qn.Ph.) was studied by spectrophotometry in the presence of a cationic, anionic, or nonionic surface-active agents in water-methanol solvents. A sensitive and simple spectrophotometric determination of uranium (VI) with Qn.Ph. and a quaternary ammonium salt was established. The dissociation equilibrium constants were respectively calculated by spectrophotometric method in water-methanol solvents with and without the presence of a surface-active agent. A spectrophotometric method for the determination of uranium (VI) based on the coloration of the uranium (VI)-Qn.Ph. complex solubilized in the cetyltrimethylammonium chloride miscelle, can be applied in the concentration range of 0-3.6μg/ml of uranium (VI). The apparant molar extinction coefficient at 555 nm is 9.4×104.
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  • MASAKI SATO, YUZO AMIKURA, SHUJI SUZUKI, TOSHIRO MURATA
    1978 Volume 98 Issue 9 Pages 1152-1157
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Effect of the soluble fraction of rat liver on lipid peroxidation in hepatic microsomes was investigated. The soluble fraction markedly accelerated the lipid peroxidation in microsomes, even in the presence of 40μM Fe2+ and/or 10μM L-ascorbic acid. However, the soluble fraction effectively inhibited the NADPH-dependent lipid peroxidation in microsomes, especially in the presence of both glucose-6-phosphate and glucose-6-phosphate dehydrogenase. The inhibitory effect on the microsomal lipid peroxidation induced by NADPH-regenerating system was reduced by heating the soluble fraction. The addition of Zn2+, an inhibitor of glutathione reductase, to the reaction mixture weakened the anti-peroxidative action of the soluble fraction on the lipid peroxide formation in microsomes together with NADPH-regenerating system and, inversely, oxidized glutathione enhanced the action of this fraction. In addition, presence of oxidized glutathione and glutathione reductase preparation was as effective as reduced glutathione in depressing lipid peroxidation in microsomes stimulated by NADPH-regenerating system. These findings suggest that both the activation of glutathione reductase system by NADPH and a heat-stable factor (s) take part in the inhibitory effect of the soluble fraction on the NADPH-linked lipid peroxidation in microsomes. This soluble fraction also inhibited the microsomal lipid peroxidation stimulated by L-ascorbic acid at the concentration of 50μM or above, and the inhibitory effect was slightly increased by heating of this fraction. It is assumed that the inhibitory effect of the soluble fraction on L-ascorbic acid-dependent lipid peroxidation might be due to the mechanism different from that acts on the NADPH-dependent lipid peroxidation.
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  • TETSUJI KAMETANI, KAZUO KIGASAWA, MINEHARU HIIRAGI, KIKUO WAKISAKA, OS ...
    1978 Volume 98 Issue 9 Pages 1158-1166
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    A variety of pyrazolo [4, 3-g] quinoline-6-carboxylic acid derivatives were prepared, for the test of antibacterial activity, by the thermal cyclization of diethyl N-(1-trityl-6-indazolyl) aminomethylenemalonate followed by alkylation and removal of the protective trityl group.
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  • HIDEO SAWADA, EMI SASAKI, SHINGO ASANO, AKIRA HARA
    1978 Volume 98 Issue 9 Pages 1167-1172
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Prostatic acid phosphatase was purified from human prostate gland by ammonium sulfate fractionation, phosphate-cellulose, Sephadex G-100, and DEAE-cellulose column chromatography. The purified enzyme was free of human serum protein and had a single protein corresponding to phosphatase activity by disc electrophoresis. Effect of various detergents and bovine serum albumin on hydrolysis of six substrates by purified prostatic acid phosphatase was investigated. Enhancing effect of various nonionic detergents and bovine serum albumin on hydrolysis of thymolphthalein monophosphate was observed. Optimal concentration of the enhancing effect was observed. In less than the optimal concentration, Lineweaver-Burk plots presented increase of Km and Vmax values, while in more than the optimal concentration Km value increased without change in Vmax value.
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  • TAKUZO HISANO, KEIJI MURAOKA, MASATAKA ICHIKAWA
    1978 Volume 98 Issue 9 Pages 1173-1182
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Oxidation with hydrogen peroxide was examined with 2-(2-pyridyl)-3-phenyl-4 (3H)-quinazolinone (Ia) and its derivatives with a methyl group in 3-position (Ib), 5-position (Ic), and 6-position (Id) of the 2-pyridyl group, in acetic acid (method A) or in trifluoroacetic acid (method B). Only N'-monoxide (II) was obtained by method A but II and N, N'-dioxide (III) were obtained by method B. (The nitrogen in 1-position of the quinazolinone ring is designated as N and that in the pyridine ring as N'). By the same reaction of 2-(4-pyridyl)-3-phenyl (or o-tolyl)-4 (3H)-quinazolinone (If or Ig), II was obtained by method A but III was not obtained by method B from which compounds formed by cleavage of the quinazolinone ring by further progress of oxidation, 2-nitrobenzanilide (IVf) and 2-nitro-2'-methylbenzanilide (IVg), were obtained, together with II. Some reactions of the N, N'-dioxides (IIIc and IIId) of Ic and Id were examined. Application of carbon disulfide or phenyl isothiocyanate to IIIc in dimethylformamide resulted in deoxygenation to form N'-monoxide (IIc) of Ic. Application of phosphorus trichloride or N, N-dimethylthiocarbamoyl chloride to IIId in chloroform also resulted in deoxygenation to form N'-monoxide (IId) of Id. Application of N, N-diethylthiocarbamoyl chloride to IId resulted in the formation of the anticipated rearrangement product, 2-{6-(N, N-diethylcarbamoylthio) methyl-2-pyridyl)}-3-phenyl-4 (3H)-quinazolinone (V).
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  • YOSHITAKA ITOH, KYOKO MINOSHIMA, KIYOMI KIKUGAWA
    1978 Volume 98 Issue 9 Pages 1183-1188
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    More than 70% of leucocytes or lymphocytes present in packed red cells were separated and recovered by cotton-wool (Gossypium barbadense) filtration. Adhering leucocytes was recovered from an Imugard filter by a plasma-containing solution with an inverted tapping. Cell viability tests as assessed by the Trypan Blue dye exclusion and phytohemagglutinin-induced transformation revealed that the recovered lymphocytes were viable.
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  • MIKIO HORI, TADASHI KATAOKA, HIROSHI SHIMIZU, KAZUHIRO ONOGI
    1978 Volume 98 Issue 9 Pages 1189-1197
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    As a fundamental study on dibenzothiepins, reaction of 11-phenyl-6, 11-dihydrodibenzo [b, e] thiepin-11-ol (10) or 11-chloro-11-phenyl-6, 11-dihydrodibenzo [b, e] thiepin (14) with triphenylmethyl cation or SbCl5 was examined under various conditions. 11-Phenyl-6, 11-dihydrodibenzo [b, e] thiepin-11-ylium salt (8) was isolated as a black powder (perchlorate) and a green powder (hexachloroantimonate), but BF4 salt was obtained only as a solution. Treatment of the hexachloroantimonate with excess of SbCl5 was found to result in a novel dehydrocyclization reaction to give selectively 9, 13b-dihydrofluoreno [1, 9a, 9-c, d] [2]-benzothiepin-13b-ylium hexachloroantimonate (19). Some discussions were made on the mechanism of the formation of 19.
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  • EIICHI KOSHINAKA, SAKAE KURATA, KAGARI YAMAGISHI, SHINJI KUBO, HIDEO K ...
    1978 Volume 98 Issue 9 Pages 1198-1207
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    For the development of a new bronchodilator, chloro-substituted α-(tert-butylamino-methyl) benzyl alcohols (VIIa-c) were synthesized via glyoxal derivatives. Further, hydroxy-substituted derivatives (VIII, IXa-e) of VIIa were synthesized by several methods. Among these compounds, o-chlorobenzyl alcohol hydrochloride (C-78) had a strong effect, and its 4-hydroxy derivative showed a strong effect, comparable to Salbutamol.
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  • AKIO ISHII, TAKAYOSHI OISHI, MORITOSHI ENDO, KAZUYUKI MINEURA
    1978 Volume 98 Issue 9 Pages 1208-1214
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Absorption, excretion, and metabolism of Glybuzole (2-benzenesulfonamido-5-tert-butyl-1-thia-3, 4-diazole) were studied in normal rats, and rats pretreated with phenobarbital, SKF 525A, or carbon tetrachloride. Plasma half-life of 14C-Glybuzole after its oral administration was affected by the pretreatment ; phenobarbital, 4.5 hr ; SKF 525A, 28 hr ; carbon tetrachloride, 34 hr ; and nontreated, 7 hr. Urinary, fecal, and biliary excretion of Glybuzole was also affected by these pretreatments. Glybuzole was excreted in urine 2.5 times higher in carbon tetrachloride-pretreated rats than in normal rats. Biliary excretion of Glybuzole in normal rats reached 91.1% of administered radioactivity within 48 hr, indicating the presence of enterohepatic circulation. Major urinary metabolites were the unchanged drug, a metabolite (I) oxidized at the tert-butyl side-chain, a novel metabolite (II) oxidized at the position para to the phenyl group of Glybuzole, and their O- or N-glucuronides. Unchanged drug was major and metabolites I and II were minor components. In bile, glucuronides, mainly N-glucuronide of Glybuzole, were major metabolites. Carbon tetrachloride pretreatment suppressed the excretion of N-glucuronide. The plasma metabolites (I and II) had no antidiabetic activity in rabbits.
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  • MASATO SHINODA, SETSUKO OHTA, YUKITOSHI HAYASE, MOTOYUKI SHIMIZU
    1978 Volume 98 Issue 9 Pages 1215-1220
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Skin damage in mice given whole-body irradiation of 30 kVp soft X-ray on the back was observed for 40 days. Depilation and ulceration were seen after irradiation of 2400-12000 R, and the time of appearance and duration of such symptoms were found to be in inverse proportion to be in inverse proportion to the irradiated dose. For irradiation of 3000-7000 R, damages quantitated from the irradiation dose and score table showed a significant linear relation. Irradiation of 4000 R showed hardly any effect on peripheral erythrocytes and total leucotytes.
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  • YOSHIYUKI SEYAMA, TAKAYOSHI TERASAWA, SABURO YAMASHITA, NOBUO ISHIKAWA
    1978 Volume 98 Issue 9 Pages 1221-1228
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Carnosine was iodinated and separated into 4-monoiodocarnosine (MIC) and 2, 4-diiodocarnosine (DIC) by Sephadex G-10 column chromatography, and their effect on smooth musole was examined. Carnosine induced smooth muscle contraction, while its iodinated products, MIC and DIC, induced relaxation of the rat intestinal duodenum. This fact suggested that a hydrogen atom at position 4 in the imidazole ring of carnosine is essential for the contraction, and its substitution by iodine produced the relaxation effect. The specificity of iodocarnosine antagonism was examined in guinea-pig ileum by inhibiting contraction induced by various agonists. It was found that MIC and DIC inhibited contraction stimulated by histamine and prostaglandine F2a(PGF2a), but they did not inhibit acetylcholine and bradykinin. This result suggested that anti-histamine effect of iodocarnosine may be ascribable to its analogous structure to histamine molecule. Therefore, other iodinated compounds having structure similar to histamine were examined for ability to inhibit histamine-stimulated smooth muscle contraction. Anti-histamine effect of monoiodohistidine (MIH) and diiodohistidine (DIH) was considerably weaker than that of β-alanyl-MIH (MIC) and β-alanyl-DIH (DIC), and monoiodohistamine did not exert anti-histamine effect at all. This suggested that at least MIH moiety is essential for the anti-histamine activity, and β-alanyl residue may augment the activity. Iodocarnosine was capable of inhibiting the contraction due to anaphylaxis in guinea-pig ileum that had been sensitized by bovine serum albumin. The anaphylatic reaction induced the release of histamine, PG's, slow reacting substance of anaphylaxis (SRS-A), and inflammatory factors in the antigen-antibody reaction. It may be concluded that iodocarnosines (either MIC or DIC) have anti-histamine, anti-PG, and anti-inflammatory effect.
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  • SHOJI OKADA, OSAMU TAMEMASA
    1978 Volume 98 Issue 9 Pages 1229-1235
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    ^<14>C-labeled dibutyl phthalate (DBP), one of the most widely utilized plasticizers, when administered orally to mice at a dose of 0.94μCi/25μg/mouse, was rapidly metabolized and excreted. The total radioactivity remaining 8 hr after the administration in 10 kinds of the organs or tissues examined was not more than 1% of the administered activity. One hour after the administration, the highest radioactivity per organ and per wet weight of tissue was detected in the liver (2.25% of the administered) and the kidney (100 dpm/mg wet tissue), respectively. In these two organs, 14C-DBP was metabolized to 14C-monobutyl phthalate (MBP) and 14C-phthalic acid, and the former was the major metabolite. Although DBP and MBP interacted with DNA in vitro, no radioactivity was recovered from the DNA of 14C-DBP-treated cells of mouse liver, possibly because 14C-DBP and its metabolites were hardly transported into the nuclei.
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  • KIKUYO TANAKA, KANETO UEKAMA, TOSHIHISA YOTSUYANAGI, KEN IKEDA
    1978 Volume 98 Issue 9 Pages 1236-1242
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Partition of ion pairs formed between phenothiazine drugs and alkylsulfonates from aqueous solution to cyclohexane was markedly enhanced by the addition of alcohols in the organic phase. Stability constants, Kc, for solvated ion pair and number of alcohol molecules associated with ion pair, n, were determined. The effect of variables, such as pH, chain length of alkylsulfonates, and temperature on the partition of phenothiazine drugs into cyclohexane containing various alcohols was investigated to gain insight into the role of alcohol solvation in enhancing the extractive process.
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  • CHIAKI TANI, NAOTAKA NAGAKURA, CHIKAKO KURIYAMA
    1978 Volume 98 Issue 9 Pages 1243-1251
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    With the droplet countercurrent chromatography, two new alkaloids, 13β-hydroxystylopine (3) and carpoxidine (9), were isolated from Corydalis ophiocarpa HOOK. et THOMS. together with many known alkaloids. Their structure was elucidated by spectroscopy and by synthesis.
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  • JOJU HAGINIWA, YOSHIHIRO HIGUCHI
    1978 Volume 98 Issue 9 Pages 1252-1254
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Lactic acid itself (or in weakly alkaline condition) is oxidized to acetaldehyde by pyridine N-oxide containing FeCl3 or FeSO4·7H2O corresponding to 1/170 mol of the acid, in N2 stream (30 ml/min). This reaction progresses at above 65° but catalytic action of iron disappears on the addition of a copper salt. This reaction carried out by the addition of potassium tert-butoxide corresponding to 1/100 mol of lactic acid to the iron salt, in an oil bath at 160-170°for 1 hr, gives acetaldehyde in 77% yield. The same oxidation in phosphoric acid acidity, without the addition of potassium tert-butoxide, gives acetaldehyde in 11% yield, with formation of pyruvic acid in 0.8% yield.
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  • TAKEHISA INOUE, TASUKU SHINBORI, MICHIYO FUJIOKA, KEIJI HASHIMOTO, YOS ...
    1978 Volume 98 Issue 9 Pages 1255-1257
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    The presence of a new pungent principle in the root of Alpinia officinarum HANCE was revealed. The structure, of this principle was elucidated as 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone [I] mainly by spectroscopic methods. The absolute configuration of I was established as (R)-form by the application of Horeau's method.
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  • TADAAKI RIKIHISA, SHINJI TOYAMA, MUTSUHIRO MORI, TOKUJI SUZUKI
    1978 Volume 98 Issue 9 Pages 1258-1266
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    The metabolic fate of o-[3-(tert-butylamino)-2-hydroxypropoxyl] benzonitrile (Bunitrolol) in the rat was studied by using the drug labeled with 14C in the nitrile group. Urine samples collected for 24 hr after oral or intravenous administration contained about 60% of the dosed 14C. The amount of unchanged Bunitrolol in urine was small after both routes of administration. The major metabolite was conjugated 4-hydroxybunitrolol, which constituted about 90% of the urinary 14C after oral administration and about 70% after intravenous administration. The amount of free 4-hydroxybunitrolol was relatively small. The sum of the recovery of 4-hydroxybunitrolol and conjugated 4-hydroxybunitrolol was almost equal after both routes of administration. Two minor metabolites were detected. One of them was conjugated 2-hydroxybenzonitrile and the other was probably 3-(2-cyanophenoxy)-2-hydroxypropionic acid. Most of the metabolites in bile was conjugated 4-hydroxybunitrolol. Blood concentration of free 4-hydroxybunitrolol in the postdistributive phase was about 25-33% of the corresponding values of the parent drug after oral administration and about 5-9% after intravenous administration. These findings were different from the fact that 4-hydroxypropranolol was detected in the systemic circulation after oral administration of propranolol but not after intravenous administration. This difference would be explained by the predominant biotransformation of bunitrolol to 4-hydroxybunitrolol.
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  • FUMIYA KUROSAKI, TAKASHI YATSUNAMI, TOSHIHIKO OKAMOTO, YOSHIYUKI ICHIN ...
    1978 Volume 98 Issue 9 Pages 1267-1273
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    A satisfactory method for quantitative determination of a series of diterpene alkaloids in Japnaese aconite roots was developed, using the combination of dual wave length thinlayer chromatography scan and gas chromatography. As an application of this method, change of each diterpene alkaloid content in some Japanese Aconitum species was examined in various seasons.
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  • SHINICHI SUZUKI
    1978 Volume 98 Issue 9 Pages 1274-1278
    Published: September 25, 1978
    Released: May 30, 2008
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    1-Phenyl-1H-pyrazolo [3, 4-d] pyrimidine-4-carboxylic acid (I) forms an ester (III) with alcohols, in the presence of an acid. III reacts with hydroxylamine, hydrazine, and amines to form hydroxamic acid, hydrazide, and amides, respectively. I also forms a labile acid chloride (V) with thionyl chloride, and V reacts with alcohols, amines, and thioalcohols to form esters, amides, and thioesters, respectively. On the other hand, I easily undergoes decarboxylation to form 1-phenyl-1H-pyrazolo [3, 4-d] pyrimidine (VII). I undergoes the Hammick reaction, and decarboxylation by heating in the presence of a carbonyl compound affords a carbinol derivative (IX) or aryl 1-phenyl-1H-pyrazolo [3, 4-d]-pyrimidin-4-yl ketone (X) formed by oxidation of IX.
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  • ISAO TAKEUCHI, ISAO OZAWA, TOSHIRO OGAKI, YOSHIKI HAMADA, TOMIYOSHI IT ...
    1978 Volume 98 Issue 9 Pages 1279-1285
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    2-Aminoquinoline and 3-amino-1, 8-naphthyridine (XI) were cyclized by ethoxy methylenemalonate and both afforded an angular type products (II and XIII). Hydrolysis of II, ethyl 4-hydroxy-5-methyl-1, 6-phenanthroline-3-carboxylate, and XIII gave 2-aminoquinoline from II and carboxylic acid derivatives (III and XIV) from the others. N-Alkylation of III, ethyl carboxylate of III, and XIV gave IV, VII, and XV. The active methylene group in XVI was oxidized with selenium dioxide to the aldehyde derivative (XVII) and XVII was oxidized with hydrogen peroxide to obtain a carboxylic acid derivative (XVIII). Antibacterial test of the synthesized compounds, III to VII, XV to XVIII, and diazaphenanthrenes (XIX to XXXIII) indicated that these compounds have a slightly strong antibacterial activity against Pseudomonas aeruginosa.
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  • KAZUO ITO, JENGSHIOW LAI
    1978 Volume 98 Issue 9 Pages 1285-1287
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    From the herbs of Marsdenia tinctoria var. tomentosa MASAMUNE (Chinese name : Ron Mao Furonran ; Asclepiadaceae), seven constituents were isolated and identified as lupenyl palmitate, lupenyl acetate, lupenone, lupeol, β-sitosterol, 5, 7-dihydroxy-2, 6, 8-trimethylchromone, and 2, 6-dimethoxybenzoquinone.
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  • SHIN MATSUURA, SHIRO YOSHIOKA, MUNEKAZU IINUMA
    1978 Volume 98 Issue 9 Pages 1288-1291
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Seventeen compounds were isolated from Cassia obtusifolia L. (Leguminosae) ; triacontan-1-ol, stigmasterol, β-sitosterol-β-D-glucoside, friedelin, palmitic acid, stearic acid, succinic acid, d-tartaric acid, uridine, myo-inositol, d-ononitol, kaempferol, quercetin, juglanin, astragalin, quercitrin, and isoquercitrin were identified with the respective authentic samples.
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  • TETSUJI KAMETANI, KAZUO KIGASAWA, MINEHARU HIIRAGI, KIKUO WAKISAKA, SE ...
    1978 Volume 98 Issue 9 Pages 1291-1296
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    In order to evaluate their antimicrobial activity, 6- and 7-carbamoyl-1-ethyl-1, 4-dihydro-4-oxoquinoline-3-carboxylic acid derivatives (2) were prepared. Thermal cyclization of diethyl N-(4- and 3-carbamoylphenyl) aminomethylenemalonates gave 6- and 7-carbamoylquinolines, which, on alkylation followed by hydrolysis, afforded 2.
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  • ATSUO NAGAMATSU, SHINJI SOEDA, JINICHI INOKUCHI
    1978 Volume 98 Issue 9 Pages 1296-1299
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    Activity of angiotensin I-converting enzyme [EC 3.4.15.1] was assayed by chromatographical separation of the synthetic substrate (Bz-Gly-Gly-Gly or Bz-Gly-His-Leu) and the cleaved product (Bz-Gly) using high-speed liquid chromatography (HLC). Bz-Gly from each unhydrolyzed substrate was separated on the column of Zipax SAX (50 cm×2.1 mm i.d.), and with 0.01 M potassium phosphate buffer (pH 6.27) as eluant. In this condition, 1.3×10-4 to 2.4×10-3 units of the enzyme activity was measurable when Bz-Gly-Gly-Gly was used as the substrate, and 3.3×10-4 to 6.0×10-3 units of the activity was assayed with Bz-Gly-His-Leu as the substrate.
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  • YOSHINOBU AKADA, SADAKO KAWANO, YAICHIRO TANASE
    1978 Volume 98 Issue 9 Pages 1300-1304
    Published: September 25, 1978
    Released: May 30, 2008
    JOURNAL FREE ACCESS
    A rapid estimation procedure using a high-speed liquid chromatography is described for determination of ammaranth, tatrazine, Sunset Yellow FCF, Erythrosine, Phloxine B, Rose Bengal, and Acid Red. Separation can be achieved within 15 min employing a 50 cm column packed with Permaphase ODS, using gradient from 0.05% cetyltrimethylammonium bromide in 20% MeOH to MeOH as an eluant with a Shimadzu Du Pont LC-2 liquid chromatograph. Accuracy in this method was found to be less than ±1.5%. The limit of detection was several nanograms. Colouring matter in gelatin capsules were examined by this analytical method. One empty capsule is dissolved in 5 ml of 0.1 N NaOH and the mixture is passed through a column (1×5 cm) containing active charcoal. Gelatin is removed by passing 100 ml of H2O through the column. The colouring matter are then eluted with 50% pyridine, the eluate is evaporated to dryness, the residure is dissolved in a small amount of H2O and examined by this method.
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