In 1949, we, working with Sauton's Synthetic-Medium Old Tuberculin (or SOT) of human stain H
2 as the source, were able to establish a simple procedure for preparing o-aminophenol azo-tuberculin derivative quite equivalent in skin-reacting potency to the PPD of Seibert (1, 2, 5) . Following this, it was also demonstrated in our Institute that all of the o-aminophenol azo-tuberculin samples derived from different SOT-lots of H
2 strain were equally effective in eliciting skin reaction on the one hand (3, 4, 7), and that para-aminophenol azo-tuberculin prepared under similar conditions also possessed of a strong skin-reacting potency comparable to that of the ortho-aminophenol azo tuberculin derivative on the other (6) .
Recently, Yanagisawa and Asami have carried out skin-test experiments on humann being with o-aminophenol azo-tuberculin derivative isolated under conditions similar to those we have used. In agreement with our observations, Yanagisawa and co-workers. found that 0.1γ of o-aminophenol azo-tuberculin derivative (human strain “Aoyama B”) was found to be equivalent to 0.05 “mg” (1/2, 000, 0.1 cc) of Old Tuberculin (8) .
It was, anyways, of interest to determine whether the skin-reacting potency of o-aminophenol azo-tuberculin derivative varied with different strains of
Mycobacterium tuberculosis or not. The present communication records the results of such experiments.
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