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Volume 16 (1963)
- Issue 6 Pages 315-
- Issue 5 Pages 251-
- Issue 4 Pages 151-
- Issue 2-3 Pages 55-
- Issue 1 Pages 1-
Predecessor
Volume 16, Issue 1
Displaying 1-6 of 6 articles from this issue
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G. M. DACK1963 Volume 16 Issue 1 Pages 1-12
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (1251K) -
TOHRU TOKUNAGA, TOYOHO MUROHASHI1963 Volume 16 Issue 1 Pages 13-20
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThese years, not a few studies have been reported on mycobacteriophage from the standpoint of phage typing (Penso et al., 1949; Hnatoko, 1953; Froman et al., 1954, 1959; Takeya et al., 1957, 1959; Doke, 1960; Hikawa, 1959; Segawa et al., 1960; Redmond et al., 1960; Murohashi et al., 1959, 1961) . Almost all of the phages studied were isolated from soil samples, and the studies were carried out independently by researchers of different laboratories according to their own methods. Differences in the biological properties between these phages were not yet sufficiently analysed in comparison. To identify or classify Mycobacteria by the use of phages isolated in various parts of the world, it seems methodologically indispensable to select adequate phage strains by their biological properties.
In the present paper, results of serological studies on the classification of 32 mycophage strains are presented. Among these phages 19 strains were sent by the courtesy of G. Penso, S. Froman, P. Hauduroy, K. Takeya and S. Doke and the remaining 13 were isolated by the present authors (Murohashi et al., 1961) . Further, based on the results obtained, phages were selected temporarily which seemed to be suitable for the typing of Mycobacteria in the present stage of the mycophage studies.View full abstractDownload PDF (793K) -
TOHRU TOKUNAGA, TOYOHO MUROHASHI1963 Volume 16 Issue 1 Pages 21-30
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe most reliable method for the phage sensitivity test of bacteria seems to be the comparison of EOP (efficiency of plating) according to the plaque counting results by the agar layer method. However, this method is relatively complicated from the technical view point, and therefore, a method of spotting a certain amount of phage suspension on the lawn of bacilli to be tested has been established and adopted routinely on Salmonella and Staphylococci, etc. In the techniques of the spotting method in these bacteria, it was known that the use of the“routine test dilution”of the typing phage is an important principle. However, in the case of Mycobacteria, very little has been hitherto reported on the methodology of the phage sensitivity test; no investigator has used the routine test dilution except Penso (1951) who applied it to saprophytic Mycobacteria.
In the present paper a routine procedure for the phage sensitivity test of Mycobacteria, the growth of which are remarkably slower than the non-acid-fast bacilli, was studied, especially the availability of a routine test dilution (RTD) of phage suspension. In addition, the following factors affecting the test results were studied: the inoculum amount of bacteria to be tested, time intervals from bacterial inoculation to phage spotting and from phage spotting to reading results.View full abstractDownload PDF (1057K) -
YUICHI KOSEKI, SHIGEHIRO OKAMOTO1963 Volume 16 Issue 1 Pages 31-38
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSCapreomycin, originally called capromycin, is a new antibiotic which was isolated from Streptomyces capreolus in the laboratories of Eli Lilly & Co. Herr et al. (1961, 1962) reported that this peptide antibiotic, with a molecular weight of approximately 740, was active primarily against Mycobacteria, and cross-resistance was not recognized between capreomycin and streptomycin or viomycin in vitro. On the other hand, Robinson and Wichelhausen (1962) noted some differences in the susceptibility to capreomycin of the wild strains tested. These reports, however, did not include a detailed analysis of the cross-resistance with capreomycin.
This paper presents results of cross-resistance experiments on capreomycin and some clinically important antimycobacterial agents, employing human tubercle bacilli and M. phlei.View full abstractDownload PDF (643K) -
TONGCHAI PAPASARATHORN, BANCHONG TONGKOOM, SUTHAM HIRANIRAMON, JIRO IT ...1963 Volume 16 Issue 1 Pages 39-43
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSchistosoma spindale (Montgomery, 1906) has never been reported in Thailand, since this worm was discovered by Montgomery (1906) in two plain cattle (Bos indices) at Muktesar in India. Liston and Soparkar (1918) completed its life cycle in the goat infected with one kind of furcocercous cercariae obtained from a fresh water snail, Planorbis exustus (synonym of Indoplanorbis exustus) .
In 1960 and 1961, the cercarial fauna of fresh water snails in Thailand was surveyed by the authors at the Department of Parasitology, Faculty of Public Health in Bangkok. During that time, as reported by Ito et al. (1962), it was revealed that a fresh water snail, Indoplanorbis exustus, from north-east provinces of Thailand was heavily infected with one kind of schistosome cercaria. This cercaria was morphologically identical with that of Schistosoma spindale, which have been known as an important blood fluke of cattle, buffalos in India, and as a dermatitis producing cercaria for the inhabitants in Malaya.
The first step of our study was focused on getting adult worms by using this cercaria through the experimental infection. After many trials during a two years' period, several adult worms were obtained from experimental animals. The followings are a preliminary report on those experiments and the identification of worms.View full abstractDownload PDF (2326K) -
1963 Volume 16 Issue 1 Pages 45-53
Published: 1963
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (849K)
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