Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
Volume 19, Issue 1
Displaying 1-7 of 7 articles from this issue
  • HIROTO SHIMOJO, KYOKO SAWAMURA, RISAKO WADA, EIKO YOSHIKAWA
    1966 Volume 19 Issue 1 Pages 1-8
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The infectivity of adenovirus type 12 and type 18 could be titrated sensitively and accurately by the use of human embryonic kidney cell cultures. Both tube titration and plaque titration could be used. The detailed techniques of the titrations are described.
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  • HIROTO SHIMOJO, HIROSHI YAMAMOTO, EIKO YOSHIKAWA, TADASHI YAMASHITA
    1966 Volume 19 Issue 1 Pages 9-22
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Tumor antigen in hamster tumors induced by adenovirus type 12 could be assorted by the complement-fixation test into two antigens: TS antigen, nonprecipitable by ultracentrifugation, and TP antigen, precipitable by ultracentrifugation. Tumor antigen was formed prior to the formation of infectious mature virus, viral coat protein, viral DNA or viral antigens in the lytic infection of human embryonic kidney cell cultures with adenovirus type 12. In the non-lytic infection of cultured hamster cells with adenovirus type 12, tumor antigen was formed as early as in human embryonic kidney cell cultures, but no infectious virus was formed. On the basis of these findings, the significance of tumor antigen in virus infection and the relationship between the virus infection and the transformation of hamster cells are discussed.
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  • HIDEMASA OGAWA, SHIGEO HONJO, MASAO TAKASAKA, TOORU FUJIWARA, KIYOSHI ...
    1966 Volume 19 Issue 1 Pages 23-32
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The locality of mulplication of S. flexneri 2a in the alimentary tract and the causal relation between the distribution of the fluorescing bacilli in the tissue and the appearence of inflammatory response were investigated on 4 cynomolgus monkeys in the earlier stage of 12 and 24 hr after an oral administration of the bacilli.
    The results of the present observations suggested the importance of the involvement of the large intestine, particularly of epithelial cells, for the multiplication of the bacilli. In the small intestine, there was evidence neither of penetration of the bacilli into the epithelial cells nor of a pronounced increase in the number of the bacilli in the lumen.
    The degeneration of the epithelial cells had already taken place when the bacilli penetrated into the cells. This fact would suggest that some preconditioning on epithelial cells by certain cytotoxic substances seems to be necessary for the penetration of the bacilli into them.
    The penetration of the bacilli into the lamina propria via the intact epithelial barrier was not observed in the earlier stage of infection; this phenomenon appeared 3 to 4 days after infection when the surface mucosa degenerated to necrosis and desquamated.
    The presence of the bacilli does not necessarily coincide with the locality of the lesions.
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  • KATSUMOTO UEDA, YOKO UEDA, KIYOSHI IMAIZUMI
    1966 Volume 19 Issue 1 Pages 33-43
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Endotoxin sensitivity was investigated in mice infected with Brucella abortus. A hyperreactive state was observed at 12 hr after intravenous injection with a large dose of Brucella.
    Ability of endotoxins of a variety of bacterial species to elicit endotoxin hyperreactivity was compared in mice infected with Brucella 24 hr previously. Endotoxins of enteric bacteria were most efficient, that of virulent Brucella was moderately efficient, and that of low-virulent Brucella was not efficient.
    The hyperreactive state after injection with different doses of 3 strains of Brucella abortus, virulent, low-virulent and avirulent, was compared. A close correlation was found between hyperreactivity to endotoxin and the severity of infection. Appearance and degree of endotoxin hyperreactivity depended on virulence and dose of Brucella injected.
    A slight degree of hyperreactivity was observed 24 hr after the injection with live avirulent Brucella or formalin-killed Brucella. Intravenous and intraperitoneal injections were much more effective than infections from other routes. Relationship between induction of endotoxin hyperreactivity and deposition of brucellae in the liver and spleen is discussed.
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  • KATSUMOTO UEDA, YOKO UEDA, KIYOSHI IMAIZUMI
    1966 Volume 19 Issue 1 Pages 45-49
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Effects of treatment with streptomycin or of pretreament with attenuated Brucella on the induction of endotoxin hyperreactivity in mice infected with Brucella abortus were investigated.
    The mice treated with streptomycin in an early stage after the infection showed a reduced sensitivity to endotoxin than control (infected, non-treated) mice, and it was also exhibited that they had fewer oreanisms in the organs than the controls.
    Mice pretreated with attenuated Brucella and challenged with a virulent strain exhibited a higher sensitivity than controls (infected) at 1 day after the challenge, but at 7 days after challenge, they showed a lower sensitivity than the controls. Viable count of the liver of the pretreated mice was reduced drastically at 7 days after challenge.
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  • ISAMU SAWADA
    1966 Volume 19 Issue 1 Pages 51-57
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Vampirolepis isensis n. sp. isolated from the bats, Rhinolophus cornutus and R. ferrumequinicm, in Mie Prefecture is described. A list of bat tapeworms reported from various districts of the world and a table of the morphological features of tapeworms in Vampirolepis are given.
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  • YOSHITAKA KOMIYA, AKIO KOBAYASHI
    1966 Volume 19 Issue 1 Pages 59-64
    Published: 1966
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
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