巻号一覧
21 巻, 6 号
選択された号の論文の7件中1～7を表示しています
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阪口 玄二, 阪口 澄子, 近藤 久1968 年 21 巻 6 号 p. 369-378
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーA bioassay method for Clostridium botulinum type-E toxins has been proposed. The method consists of intravenous injections into mice with serially diluted toxin samples and a reference toxin, determination of the time in minutes from injection to death of each mouse, conversion of the time into an appropriate score, and calculation of the toxicity relative to that of a stable, wellstandardized reference toxin by the parallel-line-assay method. If five mice are injected with each of four to five serial twofold dilutions, the fiducial limits of the toxicity will be about ±27-30%. This method enables us to determine the toxicities of several samples in an hour or two, and the relative value to a reference toxin is highly reproducible. The proposed method to calculate ip LD50 from the death time is applicable only to the trypsin-activated toxin in the undissociated as well as dissociated forms containing about 500 or more ip LD50/ml.抄録全体を表示PDF形式でダウンロード (739K) -
I. PURIFICATION AND TOXOIDING OF ALPHA TOXIN OF C. PERFRINGENS伊藤 明治1968 年 21 巻 6 号 p. 379-391
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーα-Toxin of Clostridium perfringens was highly sensitive to formalin; its antigenicity was destroyed rapidly during toxoiding. It was succeeded in toxoiding α-toxin under a strict regulation of protein concentration, formalin concentration, constituents of the medium, temperature and incubation period.
The ratio of egg unit to mouse toxicity changed during toxoiding and a long incubation was required when the egg yolk reaction was used as a measure for the toxicity. The customary usage of the in vitro test may have been one of the reasons for the inconsistent antigenicities of α-toxoid in the past.抄録全体を表示PDF形式でダウンロード (3605K) -
山内 一也, 速水 正憲, 福田 朗子, 小船 富美夫1968 年 21 巻 6 号 p. 393-404
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーResponse of Japanese quails to an inoculation of Schmidt-Rup-pin strain of Rous sarcoma virus (SR-RSV) was examined. The quails were as susceptible as chick embryo cells to the infection of SR-RSV. The tumors induced in the quails contained a significant amount of infectious virus and avian leukosis group-specific CF antigen at early stages of 1 to 3 weeks after inoculation, but they turned to be noninf ective at the late stage with a concomitant disappearance of CF antigen. High incidence of regression was observed with the tumors induced by a low dose of virus, while the tumors induced by a high dose rarely regressed.
The quails became resistant to a subsequent challenge following a primary inoculation of the virus. The resistant state was established about 10 days after a primary inoculation, and was specifically induced by infectious SR-RSV. An association of virus neutralizing antibody or interferon in the resistant state was not demonstrated. Possibility of a participation of cellular immunity in the induction of resistance was discussed.抄録全体を表示PDF形式でダウンロード (1105K) -
金井 興美, 近藤 瑩子1968 年 21 巻 6 号 p. 405-414
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーA large-molecular mycobactericidal fraction associated with cathepsin and acid phosphatase activities was separated from detergent-extracts of the lung and spleen granular fraction of tuberculous mice by gel-filtration on Sephadex G-150 column. The mycobactericidal activity was expressed only in the acidic environment below pH 6.0, and the activity was reduced when the amount of bacilli exposed to the fraction was increased. The latter observation appeared compatible with the finding that the mycobactericidal activity was removed from the fraction by absorbing with heat-killed tubercle bacilli. Heating of the fraction at 100 C and pH 5.6 for 10 min destroyed both enzymatic activities completely, but the mycobactericidal activity remained unaffected. Incubation of the fraction at 40 C and pH 4.1 liberated the mycobactericidal moiety as a smaller-molecular protein separated from acid phosphatase activity. These findings and others suggested that the mycobactericidal principle existed in the form of a complex with lysosomal hydrolases, but as a factor distinct from them.抄録全体を表示PDF形式でダウンロード (910K) -
金井 興美, 近藤 瑩子1968 年 21 巻 6 号 p. 415-422
発行日: 1968年
公開日: 2010/12/08
ジャーナル フリーAn attempt was made to compare the antimycobacterial fraction of the spleen and lung lysosomal components between normal and tuberculous mice. The results indicated that the yield of spleen mycobactericidal fraction increased after infection in terms of tissue weight and more markedly in terms of organ, and that the fraction was highly active in reducing viable counts in acidic buffer environment. As for the corresponding fraction of the lungs, the rate of infection-induced increase was not so marked as the previous case, besides the fractin was much less active even in the higher protein concentration than the spleen sample. These observations were compatible with the finding that tubercle bacilli could multiply and persist more freely in the lungs than in the spleen. In addition, it was demonstrated that tubercle bacilli exposed in vitro to the spleen antimycobacterial fraction were less infective when they were injected into mice. The significance of lysosomal response was discussed in relation to tuberculous infection and immunity.抄録全体を表示PDF形式でダウンロード (742K) -
赤真 清人, 大谷 昌, 亀山 昭一1968 年 21 巻 6 号 p. 423-426
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (398K) -
勝部 泰次, 萩原 敏且, 宮川 博, 武藤 健, 今泉 清, 増田 敬三, 三宅 巌1968 年 21 巻 6 号 p. 427-430
発行日: 1968年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (449K)
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