Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 23, Issue 5
Displaying 1-5 of 5 articles from this issue
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  • A SUGGESTED ROLE OF THE LYSOSOMAL MEMBRANE AS A PART OF THE DEFENCE MECHANISM AGAINST TUBERCULOUS INFECTION
    KOOMI KANAI, EIKO KONDO
    1970 Volume 23 Issue 5 Pages 295-302
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.295
    JOURNAL FREE ACCESS
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    A large-molecular complex fraction associating with cathepsin and acid phosphatase activities was separated from a detergent-extract of the lysosomal pellet of lungs by gel-filtration. When the fraction was obtained from“BCG-stimulated”animals, it showed antimycobacterial activities. The corresponding fraction from normal animals did not. By further fractionation, the complex was separated into 5 subfractions; hydrophobic protein, phospholipid, neutral fat, free fatty acid and water-soluble peptide fractions. The antimycobacterial activities were attributed to the last two subfractions. The corresponding subfractions from the inactive complex of normal animals were also antimycobacterial. These observations, together with those reported in the accompanying paper, led us to a view that the lysosmal membrane might be a site of the host-parasite interaction, and that a change in the orientation of membrane components caused by infection or allergic reaction produces a situation that the intracellular bacilli might come into contact with such antibacterial molecules. However, other possibilities were not excluded.
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  • SEPARATION AND PROPERTIES OF “IN VIVO GROWN TUBERCLE BACILLI” ASSOCIATED WITH THE LYSOSOMAL MEMBRANE
    KOOMI KANAI, EIKO KONDO
    1970 Volume 23 Issue 5 Pages 303-314
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.303
    JOURNAL FREE ACCESS
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    “In vivo grown tubercle bacilli”were separated mechanically from infected mouse lungs. The bacilli, unlike the same strain grown in vitro, were hydrophilic. The former were different from the latter by lowered activities of acid phosphatase at pH 6.5 and of tetrazolium-reduction, and also by new activities of acid proteinase and of acid phosphatase functional at much lower pH. Combination of Gomori stain for acid phosphatase at pH 5.0 and Ziehl-Neelsen stain revealed the association of the in vivo bacilli with membraneous tissue components with Gomori-positive reaction prolucts and some of the bacilli themselves stained black. Such observations were never obtained with in vitro grown bacilli. When the in vitro grown bacilli were incubated with lysosomal components separated from the mouse tissue, the surface of the bacilli showed a strong affinity to the lysosomal components, and in vitro tests demonstrated that the enzymatic activities of the bacilli changed into the pattern of the in vivo bacilli. The water-soluble peptide fraction and the free fatty acid fraction of the lysosomal components were found responsible for lowering the activities of tetrazolium-reduction and acid phosphatase specific for the bacilli.
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  • ANALYSIS OF HOST-ORIGINATED LIPIDS ASSOCIATING WITH “IN VIVO GROWN TUBERCLE BACILLI”
    EIKO KONDO, KOOMI KANAI, KENJI NISHIMURA, TORU TSUMITA
    1970 Volume 23 Issue 5 Pages 315-326
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.315
    JOURNAL FREE ACCESS
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    1) In order to confirm the preceding observation that “in vivo grown tubercle bacilli” harvested from infected mouse lungs were closely associated with host components, lipid analysis was conducted of this material.
    2) The 90% acetone extract contained sphingomyelin as the major phospholipid, the neutral lipids including cholesterol in 20%, and free fatty acids such as palmitic, stearic, oleic and linoleic acids.
    3) The 90% acetone-insoluble residue was further extracted with chloro-form-methanol (2: 1, v/v) . From this extract, sphingomyelin and lecithin were demonstrated only in a slight amount, insteads cardiolipin was detected as the major phospholipid. Tuberculostearic acid was present in 25% of the constituent fatty acids of the phospholipid fraction. Cholesterol was still detected, but mostly as the ester.
    4) These results, together with those in the preceding paper, suggest that the 90% acetone treatment could remove the major portion of host-originated lipids associating with the bacilli grown in vivo and the chloroform-methanol extracted mostly the components proper to the bacilli.
    5) Interesting was an additional observation that the ratio of lecithin to sphingomyelin and the ratio of cholesterol to its ester decreased greatly in the bacillus-associated host lipids, probably as a result of the host-parasite interaction.
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  • DEMONSTRATION OF MYCOLIC ACID AND PHTHIOCEROL DIMYCOCEROSATE IN “IN VIVO GROWN TUBERCLE BACILLI”
    KOOMI KANAI, ERNST WIEGESHAUS, DONALD W. SMITH
    1970 Volume 23 Issue 5 Pages 327-333
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.327
    JOURNAL FREE ACCESS
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    A study was made of the lipids of Mycobacterium bovis separated from homogenates of infected mouse lungs by differential centrifugation. By means of chromatography and infrared spectroscopy, phthiocerol dimycocerosate and mycolic acid were separated and identified.
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  • SYMPOSIUM ON TOXINS
    1970 Volume 23 Issue 5 Pages 335-379
    Published: 1970
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.23.335
    JOURNAL FREE ACCESS
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