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Volume 26 (1973)
- Issue 5-6 Pages 179-
- Issue 4 Pages 155-
- Issue 3 Pages 103-
- Issue 2 Pages 49-
- Issue 1 Pages 1-
Predecessor
Volume 26, Issue 5-6
Displaying 1-9 of 9 articles from this issue
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KUNIKO WATANABE, TOSHIO IZUMI1973 Volume 26 Issue 5-6 Pages 179-185
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe thimerosal content of serum assayed biologically (biological content) was markedly lower than that assayed chemically (chemical content) . About half of thimerosal added to fresh serum was changed immediately to a form nondialyzable against water; only half the chemical content possessed the preservative activity. The nondialyzable thimerosal content of fresh serum was higher than that of old serum; that of the albumin fraction higher than that of the globulin fraction. The nondialyzable thimerosal was separable by cysteine equilibrium dialysis without any loss of the antitoxin potencies. The present results indicate that only free, water-dialyzable thimerosal possesses the preservative activity and that thimerosal is not a suitable preservative for such protein-rich biological products as antitoxins, although about 50% of the added thimerosal retained the preservative activity for at least 10 months.View full abstractDownload PDF (574K) -
GENJI SAKAGUCHI, SUMIKO SAKAGUCHI1973 Volume 26 Issue 5-6 Pages 187-195
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe passive hemagglutination test with the sheep red blood cells coupled with Clostridium botulinum type E progenitor toxin is highly sensitive and specific to titrate type E botulinum antitoxin. The hemagglutination titers are proportional to the neutralization titers in sera obtained from immunized animals of different species, except for those taken at an early stage after the primary stimulation, when a greater part of the antibodies is in the IgM fraction.
By the use of the sheep red blood cells coupled with each component of the progenitor toxin, the antibody against each component of progenitor or activated progenitor toxin can be titrated.
A single injection with formol toxoid of the progenitor or activated progenitor toxin stimulated the animal to produce the antibody to each component at similar rates. No different immunogenicities were found between progenitor and activated progenitor toxins.View full abstractDownload PDF (696K) -
II. NEUROTROPIC PROPERTIES OF A SSPE VIRUS IN HAMSTERSSHIGETAKA KATOW, AKIRA SHISHIDO, KAORU KOBUNE, NOBUYUKI UCHIDA1973 Volume 26 Issue 5-6 Pages 197-211
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe Niigata-1 strain of subacute sclerosing panencephalitis (SSPE) virus was found highly neurovirulent in both newborn and adult hamsters as well as mice.
Rapid growth of the virus in the brain tissues of newborn hamsters was similar to that of highly neurovirulent measles virus. Pretreatment of newborn hamsters with anti-measles serum was effective on protecting them from the infection with the strain.
The growth pattern of the virus in the brain tissues of adult hamsters was quite different from that of measles viruses. The virus grew rapidly in the brain tissues of the animals, reached the maximal titer within 7 days after intracerebral inoculation, and caused deaths of the animals. On the contrary, measles virus grew once but was rapidly abolished, presumably by the effect of the antibody produced. Most adult hamsters immunized with measles virus survived the challenge with Niigata-1 strain.
Major pathological changes of the brain induced by the virus were softening, degeneration of the neurons, and cell infiltration. A case of subacute meningoencephalitis was demonstrated upon sacrifice on the 24 th day after challenge of an animal immunized with measles virus.
The relationship between neurovirulence of SSPE virus and character of cell association of the virus and between immune responses of the host to measles virus and subacute process of SSPE were discussed.View full abstractDownload PDF (8339K) -
EIKO KONDO, KOOMI KANAI1973 Volume 26 Issue 5-6 Pages 213-227
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDuring the progress of tuberculous infection in mice, cathepsin activity of the lung homogenate increased to a considerable extent. With a view that this increase was due to the local accumulation of phagocytic cells, alveolar and peritoneal macrophages were harvested for examination of their proteinase activities. They were capable of digesting acid-denatured hemoglobin and less efficiently native tuberculoproteins. Alveolar macrophages appeared more active in this respect than peritoneal macrophages. The pH optimum lay between 3.0 and 4.0. The activity was inhibited with pepstatin, a specific pepsin inhibitor, suggesting the cathepsin D-type nature. The peptide fraction as degraded products of tuberculoproteins was still capable of eliciting skin reactions in sensitized guinea pigs, though the potency was lower. These data were compatible with our previous finding that the tuberculin-active peptides of low-molecular weights were separated from the infected mouse lung homogenate.View full abstractDownload PDF (3101K) -
YOSHIKO ARIMITSU, KIYOTO AKAMA1973 Volume 26 Issue 5-6 Pages 229-237
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSPassive transfer of serum from mice infected with Borrelia duttonii showed conspicuous protection of normal mice against challenge with the pathogen. The effective principle in the serum was found in the γ-globulin fraction. On the 4th day of infection, the protective activity was detected in the 19S globulin fraction, whereas on the 30th day, mainly in the 7S globulin fraction. The circulating antibodies formed at the crisis of the disease appeared to play an important role in the disappearance of the pathogen from the circulation.View full abstractDownload PDF (767K) -
I. STAGES OF OOGENESIS AND THE FUNCTION OF THE FOLLICLEHIRONORI SAKURAI1973 Volume 26 Issue 5-6 Pages 239-248
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe ovarian development of the housefly, Musca domestics vicina Macquart was studied histologically and biochemically. During the course of ovarian development, the ovarian weight increased remarkably following the enlargement in size of the follicle; these growth curves showed similar patterns. In the developing ovary, protein and lipid accumulated abundantly, and a small amount of glycogen was observed in the later stages of oogenesis. From the cytological observations, oogenesis could be divided into 7 stages; the follicledifferentiating stage (Stage 1), the follicle-growing stages (Stages 2, 3), the vitellogenic stages (Stages 4 to 6) and the mature egg stage (Stage 7) . The oocyte initiated development at early stages of oogenesis; the nurse cells and follicular epithelial cells were considered to fulfil important functions with regard to the growth of the oocyte.View full abstractDownload PDF (4689K) -
YASUO KAWANISHI, FUMIAKI CHO, SHIGEO HONJO1973 Volume 26 Issue 5-6 Pages 249-260
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSerum protein fraction values were determined by electrophoretic separation with apparently healthy 24 laboratory-bred cynomolgus monkeys and 40 female laboratory-conditioned ones of wild origin. We found that β-globulin was always separated into two fractions. This finding seems to reflect an intrinsic nature of β-globulin of this species.
Statistically significant differences were found between young (1-2 years old) and matured (4-6 years old) laboratory-bred monkeys with regard to the total protein content and the α2-globulin value. No statistically significant difference was noted between males and females of laboratory-bred monkeys.
There was clear difference between the laboratory-bred and laboratory-conditioned monkeys in the levels of serum protein fractions; the γ-globulin value being lower and the albumin value higher in the former group than in the latter.
A probable ellipse was drawn on the basis of the combined data of the albumin and γ-globulin values. It would serve as a graphic criterion for conditioning wild imported monkeys. Its applicability was studied with groups of wildimported monkeys at various stages of conditioning after their arrival.View full abstractDownload PDF (824K) -
FUMIAKI CHO, SHIGEO HONJO1973 Volume 26 Issue 5-6 Pages 261-268
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSimplified and practical procedures for collecting macaque semen containing little coagulum and for preserving semen in vitro for a long period were studied with cynomolgus monkeys that had proved fertile.
The semen ejaculated into the vagina of the female mated was taken by aspirating with a pippet. A greater part of the semen taken in this way was usually represented by fluid.
The semen thus obtained was diluted with a glycerolated extender containing lactose, glucose, raffinose and egg-yolk. The most adequate concentration of glycerol in the extender was 5%. Then, about 0.1 ml of diluted semen was frozen into a pellet form on a dry-ice block after about 30-min pre-cooling to 4C. The frozen pellet of semen was preserved in a liquid nitrogen container. The quality of the preserved semen was evaluated by its spermatozoal survival rate and motility; the semen has successfully been preserved for at least 20 weeks until the time of preparing this paper, maintaining about a 60% level of the spermatozoal survival rate.View full abstractDownload PDF (747K) -
TOMOKO SHIMIZU, HISASHI KONDO1973 Volume 26 Issue 5-6 Pages 269-271
Published: 1973
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (287K)
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