Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 27, Issue 6
Displaying 1-4 of 4 articles from this issue
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  • ISOLATION AND CHARACTERIZATION OF AN ESTERASEACTIVE ENZYME FROM PRONASE WITH SPECIAL REFERENCE TO ACTIVATION OF CLOSTRIDIUM BOTULINUM TYPE E PROGENITOR TOXIN
    TOSHIYUKI MIURA
    1974 Volume 27 Issue 6 Pages 285-296
    Published: 1974
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.27.285
    JOURNAL FREE ACCESS
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    (1) By sequential chromatography on CM-Sephadex, SP-Sephadex and Sephadex G-100, an esterase-active enzyme was isolated from a commercial preparation of pronase, (2) The purity of the isolated enzyme was proven by disc eleetrophoresis and sedimentation analysis. (3) The molecular weight of the esterase-active enzyme was approximately 16, 000. The sedimentation coefficient was 1.85 S. The N- and C-terminal amino acids were asparagine and valine, respectively. (4) The enzyme was similar to that of bovine trypsin in the substrate specificity. It appeared to be the same as the trypsin-like enzyme isolated from pronase by Wählby (1968) . However, the relative rates of hydrolyses of different substrates by the enzyme differed from those of bovine trypsin. (5) The enzyme activated Clostridium botulinum type E progenitor toxin. Even a higher toxicity was resulted by treating the progenitor toxin with this enzyme than with bovine trypsin. (6) The results support the hypothesis that activation of type E progenitor toxin involves hydrolysis of a lysine ester bond.
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  • INTRACUTANEOUS INFECTION WITH LEPTOSPIRA ICTEROHAEMORRHAGIAE (SHIBAURA STRAIN) OF THE GUINEA PIG
    MAMORU MORI, YOSHIKO ARIMITSU, SHO OTANI, KIYOTO AKAMA
    1974 Volume 27 Issue 6 Pages 297-308
    Published: 1974
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.27.297
    JOURNAL FREE ACCESS
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    Experimental leptospirosis with Leptospira icterohaemorrhagiae Shibaura strain was studied in guinea pigs. When the pathogen was inoculated intracutaneously to the back of the animals, localized haemorrhage was observed at the inoculated site before the appearance of general haemorrhage. The severity of the local lesion increased progressively until the 7th day of inoculation. The minimum infective dose (MID) or the 50% infective dose (ID50) of the leptospiral suspension was determined by the appearance of the macroscopic local haemorrhage 7 days after inoculation. The MID thus determined was almost comparable with the value determined by the development of general symptoms and signs by conventional ip inoculation. The number of the pathogen per ID50 varied between 6 and 35 in five experiments. The local haemorrhage was effectively protected by active or passive immunization.
    Microscopically, haemorrhage at the inoculated site was found mainly in the dermis, directly beneath the epidermis in particular, and accompanied with leakage of the pathogen. The pathogen was also detected abunduntly in the thickened epidermal layer covering the inoculated area as well as in the epithelial matrix of hair-follicle, probably due to the proliferation of the pathogen at the site.
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  • A CONSIDERATION TO IMMUNE DOSES OF STAPHYLOCOCCAL ENTEROTOXIN B TO RABBITS
    KUNIHIRO SHINAGAWA, MASANORI ISHIBASHI, HIROYUKI YAMAMOTO, NOBUHARU KU ...
    1974 Volume 27 Issue 6 Pages 309-314
    Published: 1974
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.27.309
    JOURNAL FREE ACCESS
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    Immune doses of staphylococcal enterotoxin B to rabbits were studied by comparing the efficiency of various immunization schedules. It was experienced that even two subcutaneous injections each with such a small dose as 10μg of enterotoxin B, the primary one with Freund's complete adjuvant and a booster one without adjuvant, could stimulate a rabbit to develop the antibody to a satisfactorily high titer determined by agar gel diffusion and passive hemagglutination.
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  • STANDARDIZATION AND CONTROL OF TOXOID COMPONENTS IN THE COMBINED VACCINE (DPT)
    SATORU KONDO, SHOICHI KAMEYAMA, RYOSUKE MURATA
    1974 Volume 27 Issue 6 Pages 315-319
    Published: 1974
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.27.315
    JOURNAL FREE ACCESS
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