巻号一覧
28 巻, 1 号
選択された号の論文の5件中1～5を表示しています
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赤真 清人, 大谷 昌, 森 守, 有光 佳子1975 年 28 巻 1 号 p. 1-9
発行日: 1975年
公開日: 2010/03/19
ジャーナル フリーA method for estimating the leptospiricidal activity of therapeutic antiserum for Weil's disease was improved by using the intracutaneous method in guinea pigs. The neutralization curves between the leptospiral suspensions for challenge and the antisera were shown to be linear over a wide range of the dosis of the pathogen. Although the reproducibility of the neutralization experiments was high, a significant divergence was observed among the slopes in various test samples. However, the trouble due to such a discrepancy in the slope may practically be reduced if such an antiserum preparation that has a regression coefficient close to the mean of those of various neutralization lines constructed by many different products is adopted as the reference. A practical method for the potency test was suggested.抄録全体を表示PDF形式でダウンロード (799K) -
小早川 隆敏1975 年 28 巻 1 号 p. 11-22
発行日: 1975年
公開日: 2010/03/19
ジャーナル フリーCell-mediated immunity to Dirofilaria immitis (DI) in guinea pigs was confirmed by the migration inhibition test (MIT), the blast transformation test (BTT), the delayed skin reaction, and the skin reaction by passive transfer with sensitized peritoneal exudate (PE) cells. All migration inhibition (MI) positive cases were always associated with positive skin reactions and two cases showed positive skin reactions without MI. The cellular antibody confirmed by MIT first appeared on the 4th day after single sensitization, but DNA synthesis in splenic lymphocytes had already started on the 3rd day in the absence of delayed skin reaction and MI.
Then, the role of this cellular antibody in the immune mechanism against DI infection was investigated by the in vitro and in vivo cytotoxicity test using microfilariae (Mf) of this species as a target. The cytotoxic activity significantly increased in the sensitized splenic and PE cells, and in vivo normal PE cells implanted into sensitized animals.抄録全体を表示PDF形式でダウンロード (2608K) -
杉本 正信, 小島 朝人, 柳沼 一政, 江頭 靖之1975 年 28 巻 1 号 p. 23-35
発行日: 1975年
公開日: 2010/03/19
ジャーナル フリーThe mouse sensitized by subcutaneous (sc) injection of lysozyme in emulsion of Freund's complete adjuvant (FCA) was shown by a modified footpad test to develop three kinds of hypersensitivities. Injecting lysozyme in 2.5-μ1 emulsion of Freund's incomplete adjuvant (FIA) into the footpad elicited strong footpad swelling in 30 min (anaphylactic reaction), in 3 hr (Arthus-type reaction) and in 24 hr (delayed-type hypersensitivity; DTH) . The mice showing anaphylactic reaction in the footpad test manifested severe active systemic anaphylaxis, and the sera of these animals showed high IgG1 antibody titers with only sparingly detectable or no IgE antibody titers.
In the sensitizing system with the use of FCA, the antigenicity of S-carboxy-methylated lysozyme (CM-lysozyme) devoid of the three-dimensional conformation of lysozyme was compared with that of the native molecule. CM-lysozyme and lysozyme completely cross-reacted to each other in DTH, but not at all in the anaphylactic or Arthus-type reaction or in IgG1 antibody production. CM-lysozyme was shown also to have the ability to bestow immunological memory for the induction of humoral immunity against lysozyme; intravenous (iv) injection of lysozyme in saline or sc injection of CM-lysozyme-FCA alone failed to induce immediate hypersensitivities and IgG1 antibody production against lysozyme, but pre-sensitization by sc injection of CM-lysozyme-FCA enabled the animal to induce these responses to significant levels when iv injection of lysozyme in saline was given as a booster.抄録全体を表示PDF形式でダウンロード (1215K) -
黒川 正身, 石田 説而, 朝川 貞雄, 岩佐 三郎, 後藤 紀久, 倉塚 和夫1975 年 28 巻 1 号 p. 37-52
発行日: 1975年
公開日: 2010/03/19
ジャーナル フリーIntraperitoneal or intravenous inoculation of live or inactivated influenza virus induced two characteristic responses of the peripheral leukocytes in mice, an early appearing leukopenic response and late appearing lymphopenia. The former response usually developed and subsided within several hours, though the change in leukocyte population was fairly complicated depending upon the activity of the inoculated material, while the latter began several hours after inoculation and reached its minimum level in 10 to 20 hr. The agent responsible for the former may be virus pyrogen, while the latter seems to be caused by some substance (s) other than that.
The early appearing leukopenic response was similar to that due to bacterial endotoxin in respect to the characteristic pattern of the change in peripheral leukocyte population, though it was relatively easy to distinguish one from the other by the length of the latent period and by the heat stability of the causative agent. Live or inactivated influenza virus causing the early appearing leukopenic response was found also to have the mouse body weight-decreasing toxicity.
The significance of these findings in the laboratory control test of influenza vaccine for untoward reactions often observed in human inoculated with some inactivated influenza vaccines was discussed. The possible roles of the two agents, virus pyrogen and endotoxin, in the febrile response were mentioned.抄録全体を表示PDF形式でダウンロード (3027K) -
1975 年 28 巻 1 号 p. 53-100
発行日: 1975年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (4665K)
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