Allergen in crude extract of
Dirofilaria immitis was purified and separated from the IgG-inducing antigens by a combination of DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration.
The molecular weight of the purified preparation was estimated to be approximately 20, 000 by gel filtration. The carbohydrate content of the preparation was apparently low, about 2%.
Rats immunized with the allergenic fraction developed only a homocytotropic antibody and no hemagglutination antibody.
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