Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
Volume 33, Issue 4
Displaying 1-5 of 5 articles from this issue
  • ATSUKO MORIBAYASHI, TETSUYA OHTAKI
    1980 Volume 33 Issue 4 Pages 189-201
    Published: 1980
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The inactivation process of ecdysteroids during pupal-adult development of the fleshfly, Sarcophaga peregrina, was analyzed by following injected 3H-20-hydroxyecdysone. 20-Hydroxyecdysone was rapidly metabolized and converted mostly into three groups of metabolites, one of which was closely related to 20-hydroxyecdysone in its chemical nature and the other two were polar conjugates of 20-hydroxyecdysone (OA and OB) . The first group of metabolites decreased through the first day after injection of 20-hydroxyecdysone, and began to increase thereafter. Those of conjugates OA and OB increased through the first day after the injection and then began to decrease. The conjugate OA introduced into phraate pupae changed to less polar substances, liberating 20-hydroxyecdysone at the beginning of adult development. Since the conjugates OA and OB did not exhibit any appreciable moulting hormone activity, we consider that some part of 20-hydroxyecdysone synthesized during larval-pupal development is stored as conjugate forms until the beginning of adult development, and that the conjugate OA is converted to 20-hydroxyecdysone for adult development.
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  • HIROSHI FUJIWARA, KAZUO KURATSUKA
    1980 Volume 33 Issue 4 Pages 203-216
    Published: 1980
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The body weight-decreasing effect of sublethal doses of endotoxin was enhanced in mice administered with such an amount of lead acetate that had no effect by itself on body weight. The enhancing effects of administration of lead acetate prior to, simultaneously with and after endotoxin administration were analyzed and compared by statistical methods.
    Linear dose-response relation between logarithmic doses of endotoxin and body weight-decreasing responses was obtained over a wide range of endotoxin doses with a constant dose of lead acetate. The non-parallelism between two dose-response lines obtained in lead acetate-treated and control mice was not denied. The relation of the two lines indicated that the minuter the dose of endotoxin, the more intense the enhancing effect of lead acetate appearing in the treated mice within certain limits of minute doses of endotoxin. These results were common in the three administration methods of lead acetate and can be applied to detection of minute doses of endotoxin.
    It was suggested that the pre-administration method of lead acetate was more suitable for detection of endotoxin than the other methods.
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  • SUHAS C. SANYAL, RAJIV K. AGARWAL, ESWARA ANNAPURNA, JOHN V. LEE
    1980 Volume 33 Issue 4 Pages 217-222
    Published: 1980
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Recently a group of organisms resembling Aeromonas hydrophila has been isolated from human diarrheal cases and from marine and estuarine environment. These organisms are tentatively designated as group‘F’vibrios. Live cells of 23 strains isolated from different sources were tested for their enterotoxicity in the adult rabbit ileal loop model. All but two caused fluid accumulation comparable to that obtained with Vibrio cholerae 569B. An inoculum of 104 viable bacteria could give rise to gut loop reaction. Culture filtrates of the 19 strains tested including one giving negative reaction with live cells also caused accumulation of fluid in loops.
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  • YOSHIKO ARIMITSU, MAMORU MORI, KIYOTO AKAMA
    1980 Volume 33 Issue 4 Pages 223-229
    Published: 1980
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Cross protective antigenicities of Leptospira were studied with 16 antisera to 13 serovars of Leptospira interrogans and a virulent strain “Shibaura (v) ”, which is now considered to belong to serovar copenhageni, for challenge. The antisera of the rabbits highly immunized with various serovar live antigens were examined for leptospiricidal activity; cross immunity was recognized only in the same serogroup, Icterohaemorrhagiae.
    Serovar icterohaemorrhagiae lacks a part of the antigenic components of copenhageni, but the antiserum to the former showed potent leptospiricidal activity to Shibaura (v) . Anti-copenhageni immune serum absorbed with the icterohaemorrhagiae antigen did not show any protective activity to Shibaura (v) .
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  • AKIRA SHIRAI, ALEXANDER L. DOHANY, ELSIE GAN, TEIK CHYE CHAN, DAVID L. ...
    1980 Volume 33 Issue 4 Pages 231-234
    Published: 1980
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Fifty-one Rickettsia tsutsugamushi isolates from small mammals collected in central Peninsular Malaysia were serologically characterized by direct immunofluorescence using eight prototype strains. Karp and Karp-related (TA763, TA686, TA716) antigens were found in 90.2% of the isolates.
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