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Volume 34 (1981)
- Issue 6 Pages 329-
- Issue 5 Pages 271-
- Issue 4 Pages 197-
- Issue 3 Pages 113-
- Issue 2 Pages 61-
- Issue 1 Pages 1-
Predecessor
Volume 34, Issue 6
Displaying 1-6 of 6 articles from this issue
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RYOSUKE MURATA1981 Volume 34 Issue 6 Pages 329-354
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (1947K) -
TAKESHI A. SATO, MASANORI HAYAMI, KAZUYA YAMANOUCHI1981 Volume 34 Issue 6 Pages 355-364
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSerological relationships among measles virus (MV), canine distemper virus (CDV), and rinderpest virus (RV), which constitute morbillivirus subgroup of paramyxoviridae, were investigated by immunoprecipitation and SDSpolyacrylamlde gel electrophoresis for their major structural proteins, i.e., hemagglutinin (H), nucleocapsid (NC), fusion (F), and matrix (M) proteins. The molecular weights of the four structural proteins of MV and CDV were confirmed to correspond to those previously reported by several investigators. Structural proteins of RV were analyzed for the first time in the present study and found to have molecular weights of 74, 000, 62, 000, 44, 000, and 40, 000 for H, NC, F, and M proteins, respectively. By labeling with glucosamine, the presence of carbohydrate moiety was found in H protein for all the three viruses and in F protein of CDV. The serums from the convalescent animals infected with respective virus disclosed one-way cross pattern depending on the combinations of virus and antiserums, but failed to show the reciprocal cross reactivity. On the other hand, hyperimmune serums to respective virus showed the reciprocal cross-reactivity with the four structural proteins indicating that each of the major structural proteins possesses the antigen common to all three morbilliviruses.View full abstractDownload PDF (2154K) -
TAKESHI A. SATO, MASANORI HAYAMI, KAZUYA YAMANOUCHI1981 Volume 34 Issue 6 Pages 365-373
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSBy immunoprecipitation and SDS-polyacrylamide gel electrophoresis, antibody responses to the structural proteins of measles virus were examined on patients with various forms of natural measles, atypical measles, and subacute sclerosing panencephalitis (SSPE) . The serum of atypical measles most strongly reacted with four structural proteins, i.e., hemagglutinin (H), nucleocapsid (NC), fusion (F), and matrix (M) proteins. In natural measles, antibodies to the four structural proteins were detected at such an early convalescent stage as one month after the onset of disease. In late convalescent serums taken 9 years after natural measles, antibodies to the four structural proteins were detected at such an early convalescent stage as one month after the onset of disease. In late convalescent serums taken 9 years after natural measles, however, only low level antibody to M protein was present, whereas antibodies to H, NC and F proteins persisted. The serums and cerebrospinal fluid of SSPE patients showed patterns similar with those of the late convalescent serums. In Vero cells infected with cell-associated SSPE viruses (Niigata-1, ZH, and SI strains), M protein was not clearly demonstrated with serum of either atypical measles or SSPE patients, whereas H protein was demonstrated.View full abstractDownload PDF (790K) -
ROJANASUPHOT SUNTHAREE, CHANYASANHA CHARNCHUDHI, AHANDRIK SOMPOP, CHAT ...1981 Volume 34 Issue 6 Pages 375-379
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDengue viruses were isolated in a mosquito cell clone, C6/36, and their serotypes were identified by the immune adherence hemagglutination (IAHA) test. Even when viruses were not cytopathogenic, the IAHA test detected virus growth in the cells.View full abstractDownload PDF (307K) -
AKIRA SASAGAWA, TOYOKO INAYOSHI, FUKUYO NAKAMURA, MAKOTO SUZUKI, SHIGE ...1981 Volume 34 Issue 6 Pages 381-384
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSOne hundred and eighty-one cynomolgus monkeys born and raised at Tsukuba Primate Center for Medical Science (TPC) were serologically examined for the presence of antibodies against simian virus 5, measles virus and herpes simplex virus. All of them were virtually free from infections with these viruses, whereas wild-originated breeders kept at TPC were highly positive for these antibodies. These results can be regarded as demonstrating that an indoor breeding system such as TPC's is useful and valuable for producing nonhuman primates of good quality being completely free from those viral infections.View full abstractDownload PDF (362K) -
NOBUYUKI SHINOHARA, HIROSHI TANAKA, TSUYOSHI SAITO, JUNKO DEGUCHI, REI ...1981 Volume 34 Issue 6 Pages 385-392
Published: 1981
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSince periodical survey of the sewage entering the sewage-farm in Matsuyama City revealed a high incidence of Salmonella typhi of different phage types, attempts were made to trace the upstream reservoir. It was found that S. typhi was drained into a particular manhole at a distance of about 5 km from the sewage-farm. Two members of two families were found to be carriers. Further investigation detected other 25 carriers. The 27 carriers were all pupils of the same primary school. Ten of them showed mild symptoms such as fever, abdominal pain and diarrhea; the remaining 17 were asymptomatic. The phage type of 24 isolates was of Vi degraded approaching phage type A [degraded Vi (A) ] and that of the other three was of type 53. The results coincided with those of the isolates from sewage.View full abstractDownload PDF (697K)
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