Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 35, Issue 3
Displaying 1-3 of 3 articles from this issue
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  • PURIFICATION AND PHYSICOCHEMICAL AND IMMUNOLOGICAL ANALYSIS OF CHICKEN ALPHA-FETOPROTEIN
    EIJI IDO, TETSUYA MATSUNO
    1982 Volume 35 Issue 3 Pages 87-96
    Published: 1982
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.35.87
    JOURNAL FREE ACCESS
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    Chicken alpha-fetoprotein was isolated from 12 to 13-day-old embryonic chicken serum by column chromatography on CM-Sephadex C-50, Hydroxyapatite and DEAF-Sephadex A-25. The purified protein was homogeneous based on polyacrylamide gel electrophoresis, immunoelectrophoresis and isoelectric focusing. The purified protein had the following physicochemical and immunological properties. (1) It was a glycoprotein with a single polypeptide chain. (2) The molecular weight of the protein was estimated at 71, 000 by SDS-polyacrylamide gel electrophoresis. (3) The isoelectric point of the protein was 4.90. (4) The amino acid composition of the protein was similar to those of mammalian α-fetoproteins. (5) The protein showed no steroid-binding capacity. (6) It was immunologically distinct from mammalian α-fetoprotein. (7) No immunological cross-reaction was observed between the protein and chicken albumin.
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  • NATURAL KILLER CELL ACTIVITIES OF SEVERAL INBRED STRAINS OF THE GUINEA PIG AND THEIR SUSCEPTIBILITIES TO RABBIT ANTISERUM DIRECTED TO GUINEA-PIG BRAIN TISSUE
    TETSURO KATAOKA, TAKAO OHNUKI, MIKIO MIYAMOTO, TOHRU TOKUNAGA, TSUNEO ...
    1982 Volume 35 Issue 3 Pages 97-104
    Published: 1982
    Released on J-STAGE: September 07, 2010
    DOIhttps://doi.org/10.7883/yoken1952.35.97
    JOURNAL FREE ACCESS
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    Natural killer (NK) cell activities of spleen and peripheral blood leukocytes from several inbred guinea-pig strains, established in this Institute, were assessed in an 18-hr 51Cr-release assay. As target cells in the NK assay, a human myeloid cell line, K562, appeared to be the most suitable among several cultured cell lines tested. Intensities of cytolysis were different from strain to strain, but generally high in JY-2, JY-4 and JY-10 and low in JY-1. Treatment of the effector cells with rabbit anti-guinea-pig brain tissue serum plus complement reduced the NK activity, whereas treatment with rabbit anti-guinea-pig thymocyte serum, or rabbit anti-asialo GM1 serum, plus complement showed no significant effect on the NK activity.
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  • SYMPOSIUM ON TOXINS
    1982 Volume 35 Issue 3 Pages 105-141
    Published: 1982
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.35.105
    JOURNAL FREE ACCESS
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