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Successor
Volume 36 (1983)
- Issue 6 Pages 299-
- Issue 5 Pages 261-
- Issue 4 Pages 199-
- Issue 3 Pages 135-
- Issue 2 Pages 67-
- Issue 1 Pages 1-
Predecessor
Volume 36, Issue 3
Displaying 1-6 of 6 articles from this issue
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NANAE IZUMI, HISASHI KONDO, IWAO OHISHI, GENJI SAKAGUCHI1983 Volume 36 Issue 3 Pages 135-145
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe α-toxin of Clostridium oedematiens type A was purified from culture filtrate by two steps of column chromatography and repeated gel filtration. The purified α-toxin proved homogeneous in polyacrylamide gel electrophoresis and agar gel double diffusion. The molecular weight of the α-toxin was estimated at 280, 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis and at 260, 000 by gel filtration on a Sephadex G-200 column. The isoelectric point determined by isoelectric focusing polyacrylamide gel electrophoresis was 6.1. No dissociation of the purified α-toxin into subunits was demonstrated in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The 50% lethal and edematizing doses per mg protein of the purified α-toxin were 5.9×104 and 5.9×105, respectively. The L+/50 doses per mg protein of the toxin was 4.6×103. The purified α-toxin, when injected intradermally into the rabbit skin, induced increased vascular permeability. The toxin contained little or no hemolytic or lecithinase activity. These results attest that the lethal, edematizing and vascular permeability-enhancing activities elicited by C. oedematiens type A culture reside on the same protein molecule.View full abstractDownload PDF (3516K) -
TAZUKO YUASA, FUMITOSHI CHINO, TAKASHI TSURUHARA1983 Volume 36 Issue 3 Pages 147-156
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSRabbits were immunized with vaccinia-soluble early antigen (Es antigen) in Freund's complete adjuvant (FCA) . Circulating neutralizing antibody was not induced, but CF antibody against the soluble antigens of vaccinia-infected cells was induced. By intradermal inoculation of vaccinia virus, erythema developed early at the site of inoculation and enlarged up to 3 days post infection (p.i.) . No multiplication of the viruses in the erythematous areas was indicated on day 3 p.i. The erythematous areas showed histologically severe changes involving infiltration of numerous cells accompanied with necrotic and hemorrhagic lesions in a part of the dermis. Eosinophilic leukocytes were dominant and there were also some lymphocytes in the cell infiltration. The role of these kinds of cells was discussed in connection with cell-mediated immunity in the mechanism of excluding vaccinia virus from the immune rabbits.View full abstractDownload PDF (3947K) -
SETSUJI ISHIDA, SABURO IWASA, KIYOTO AKAMA, SADAO ASAKAWA, MARIKO TAI, ...1983 Volume 36 Issue 3 Pages 157-170
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA method has been developed for quantitative determination of the early appearing mouse paw swelling activity of pertussis vaccine. Mice were inoculated with the vaccine into the left hind paws. The difference in the thicknesses between the inoculated and the uninoculated paws was taken as the swelling response in 16 hr after inoculation. It was transformed into logarithm as a response metameter. A linear log dose-log response regression line was obtained over a relatively wide range of doses of the vaccine. No deviation from parallelism was significant among the regression lines of different vaccines tested.
The swelling activities relative to that of a reference vaccine of diphtheria-pertussis-tetanus combined (DPT) vaccines recently produced in Japan were determined. The relative activities ranged from 0.6 to 2.5, being twice the mean width of the 95% confidence interval of the relative activity.
Further experiments showed that the activity significantly correlated with endotoxin and histamine-sensitizing factor (HSF) but not with lymphocytosis-promoting factor (LPF) . In addition, the multiple regression analysis of such relationship suggested that the swelling activity is accounted for by the combined action of endotoxin and HSF. A model experiment with a mixture of the two toxins supported this conclusion.View full abstractDownload PDF (1239K) -
KOJI FUJIMOTO, KEIJI TERAO, FUMIAKI CHO, SHIGEO HONJO1983 Volume 36 Issue 3 Pages 171-176
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe placental transfer of IgG from the mother to her fetus was investigated with colony-bred cynomolgus monkeys. Very low levels of IgG were detected in sera of 84 days old fetus, indicating that transplacental transfer of IgG had started by this fetal age. Afterwards, gradual increase in the IgG level continued till 140 days of fetal age. A marked increase in the level was noted after 140 days of fetal age, during the last 4 weeks of full gestation term. Fetal anti-measles antibody increased in a pattern similar to that in the IgG level. The IgG level and anti-measles and anti-streptolysin O antibody titers of newborns never exceeded those of their mothers.View full abstractDownload PDF (447K) -
A report of the National Epidemiological Surveillance for Important Communicable DiseasesKIKUKO MIYAMURA, RYOSUKE MURATA, REISAKU KONO1983 Volume 36 Issue 3 Pages 177-190
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSAntitoxic immunity against diphtheria was surveyed during the period from 1962 to 1980. The survey was done by Schick test in the first decade and then by antitoxin titration of sera by the cell culture method in the recent six years. The data show clearly that the successful control of diphtheria has been accomplished in Japan as the result of active immunization. Both single (D) and combined (DP or DPT) vaccines were effective to convert Schick reaction negative. In addition, immunity was greatly improved by the introduction of the combined vaccine and one additional booster injection. However, such subsequent changes in the immunization schedule introduced in 1976 that giving the primary vaccination at older age and the omission of one booster injection at the preschool, has resulted in appearance of a high risk group below three years of age as well as in the lower immunity levels among school children. The data indicate that the continuous surveillance for diphtheria is required in this country.View full abstractDownload PDF (1111K) -
NOBUYUKI SHINOHARA, HIROSHI TANAKA, TSUYOSHI SAITO, JUNKO DEGUCHI, KEN ...1983 Volume 36 Issue 3 Pages 191-197
Published: 1983
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSEighty-two cases of typhoid fever were found in Matsuyama city in the period from 1974 to 1981. Seventy-six cases were found to be infected with Salmonella typhi other three with Salmonella paratyphi A, and the remaining three were diagnosed only clinically. The strains of S. typhi isolated from these patients showed such a variety of Vi-phage types as D1, D2, E1, M1, 53 and degraded Vi-positive strain (DVS) . The concurrent survey of the city sewage and river waters for typhoid bacilli was conducted with total 578 samples taken therefrom. S. typhi was isolated from 120 of those samples. The Vi-phage types of the isolates were closely related with those of the isolates from the patients. The periodical examinations of the city sewage and the draining river may serve as a useful means for the controlling typhoid fever epidemics.View full abstractDownload PDF (594K)
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