Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
Volume 39, Issue 5-6
Displaying 1-4 of 4 articles from this issue
  • Hisaichi BANNAI
    1986 Volume 39 Issue 5-6 Pages 185-198
    Published: 1986
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Two human interferons (IFNs), natural lymphoblastoid IFN-α (Lb-IFN-α) and recombinant IFN-α (Re-IFN-αA) were compared for their induction of 2'-5' oligoadenylate (2-5A) synthetase and pharmacokinetic behaviors in cynomolgus monkeys. In in vitro experiments, (1) the enzyme activity induced in an epithelial cell strain from human amniotic membrane (FL) cells was much higher than that in primary culture of cynomolgus monkey kidney (PMK) cells, and (2) Lb-IFN-α induced higher levels of the enzyme than did Re-IFN-αA in both cells. In in vivo experiments, (1) no difference was found in clearance from the blood between the two IFNs, but the half life of the enzyme induced in peripheral leukocytes by Lb-IFN-α was about twice longer than that by Re-IFN-αA, and (2) the enzyme levels detected in various tissues were IFN-dose dependent. These results indicate that natural Lb-IFN-α is more effective in inducing 2-5A synthetase than Re-IFN-αA, and that the enzyme activity of various tissues as well as peripheral blood lymphocytes (PBL) increased in the cynomolgus monkeys administered with human IFNs.
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  • Masanori KAWANAKA, Shigeo HAYASHI, Clint E. CARTER
    1986 Volume 39 Issue 5-6 Pages 199-206
    Published: 1986
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The utilization of amino acids and glucose in the external nutrients and the excretion of nitrogenous compounds by Schistosoma japonicum eggs were investigated with the eggs cultured in a chemically defined medium (MEMSE-J) . Of the 15 amino acids in MEMSE-J, arginine and glutamine markedly decreased in concentration during cultivation of S. japonicum eggs. The nitrogenous excretory products of developing eggs were demonstrated to be at least four amino acids (alanine, proline, glutamic acid and ornithine), urea and ammonia. Glucose was consumed at an estimated rate of 32 ng/living egg/day during the period of egg growth and differentiation. When 14C-labelled glucose was included in the culture medium, the radioactivity was incorporated into three amino acids (alanine, proline and glutamic acid), which were excreted by S. japonicum eggs. The results were discussed with reference to the possible role in stimulating fibrosis in the granuloma of schistosomiasis.
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  • Soedarto SOEKIMAN, Takeo MATSUMURA, Hiroshi YAMANISHI
    1986 Volume 39 Issue 5-6 Pages 207-211
    Published: 1986
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Aedes albopictus as well as Aedes aegypti is an important vector of chikungunya and dengue viruses. Electron microscopic observations on the salivary glands of Ae. albopictus infected with chikungunya virus were performed in comparing with those of Ae. aegypti infected with dengue virus. No virus budding from the cell surface of the chikungunya-infected mosquito's salivary glands was found as shown in dengue-infected ones, in contrast to the findings of the mammalian cells such as Vero, KB, IMR, J-111 and BHK-21 cells infected with chikungunya and/or dengue virus (es) .
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  • 1986 Volume 39 Issue 5-6 Pages 216-259
    Published: 1986
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
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