The utilization of amino acids and glucose in the external nutrients and the excretion of nitrogenous compounds by
Schistosoma japonicum eggs were investigated with the eggs cultured in a chemically defined medium (MEMSE-J) . Of the 15 amino acids in MEMSE-J, arginine and glutamine markedly decreased in concentration during cultivation of
S. japonicum eggs. The nitrogenous excretory products of developing eggs were demonstrated to be at least four amino acids (alanine, proline, glutamic acid and ornithine), urea and ammonia. Glucose was consumed at an estimated rate of 32 ng/living egg/day during the period of egg growth and differentiation. When
14C-labelled glucose was included in the culture medium, the radioactivity was incorporated into three amino acids (alanine, proline and glutamic acid), which were excreted by
S. japonicum eggs. The results were discussed with reference to the possible role in stimulating fibrosis in the granuloma of schistosomiasis.
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