Japanese Journal of Medical Science and Biology

MONKEY B-LYMPHOTROPIC PAPOVAVIRUS GENOME: THE ENTIRE DNA SEQUENCE AND VARIABLE REGIONS

Akemi FURUNO, Tadahito KANDA, Kunito YOSHIIKE

1986 Volume 39 Issue 4 Pages 151-161
Published: 1986
Released on J-STAGE: March 19, 2010

DOIhttps://doi.org/10.7883/yoken1952.39.151

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DNA from monkey B-lymphotropic papovavirus (LPV) adapted to growth in human B-lymphoblastoid cell line BJA-B consisted of three classes of molecules of 5.1, 5.0, and 4.9 kilobases (kb), and the entire DNA sequence of 5.1-kb molecule was determined. The three types of LPV DNA yielded infectious virus upon transfection to BJA-B cells. Comparison of the structures among these clones and clone K38 sequenced by Pawlita et al. (1985) suggests that the transcriptional control region, the small-T antigen gene, and the VP-1 gene are variable in the LPV genome, and that the 5.0-kb LPV resembles the parent virus, from which all the others had evolved by duplication and deletion during passages in BJA-B cells, probably for adaptation to better growth in human cells.

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USE OF MICROPLATE CELL CULTURE AND ENZYME IMMUNOASSAY IN TITRATION OF SERUM NEUTRALIZING ANTIBODY AGAINST HOCHI STRAIN OF SEROTYPE 4 HUMAN ROTAVIRUS

Tomoko URASAWA, Yasuyuki MORITA, Shozo URASAWA, Koki TANIGUCHI

1986 Volume 39 Issue 4 Pages 163-167
Published: 1986
Released on J-STAGE: March 19, 2010

DOIhttps://doi.org/10.7883/yoken1952.39.163

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The tube neutralization test read by enzyme immunoassay developed by Wyatt et al. (1983) for serotype determination of human rotavirus was modified so as to use stationary cultures of MA104 cells in a microtiter plate instead of roller tube cultures. Sera obtained from different age groups were titrated for neutralizing antibody against serotype 4 human rotavirus Hochi strain by this test and the results were compared with those obtained by the plaque neutralization test. There was a good correlation between the titers obtained by the two tests and the age distribution pattern of serotype 4 neutralizing antibody was similar to those of serotype 1 and 3 antibodies previously reported.

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CYTOTOXIC AND ANTITUMOR ACTIVITY OF A SOLUBLE FRACTION OF STREPTOCOCCUS PYOGENES AGAINST S180 SARCOMA CELLS

Yoshihiro HIGUCHI

1986 Volume 39 Issue 4 Pages 169-175
Published: 1986
Released on J-STAGE: March 19, 2010

DOIhttps://doi.org/10.7883/yoken1952.39.169

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A fraction (60F) having cytotoxic and antitumor activities was obtained from cell-free extract of group A Streptococcus pyogenes by precipitating with 50% to 60% saturated ammonium sulfate. 60F showed cytotoxic activity inhibiting the uptake of ³H-thymidine by S180 sarcoma cells and enhancing ⁵¹Cr-release from ⁵¹Cr-labeled cells. 60F showed also antitumor activity, depressing tumor growth and prolonging lives of mice bearing S180 sarcoma cells.

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HUMAN CASES OF INFECTION WITH CANINE WHIPWORMS, TRICHURIS VULPIS (FROELICH, 1789), IN JAPAN

Noboru KAGEI, Shigeo HAYASHI, Keiko KATO

1986 Volume 39 Issue 4 Pages 177-184
Published: 1986
Released on J-STAGE: March 19, 2010

DOIhttps://doi.org/10.7883/yoken1952.39.177

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The eggs of Trichuris, detected in the feces of 19 persons who had stayed in institutions for mentally retarded and/or multi-handicapped patients, were identified as those of T. vulpis (Froelich, 1789) on the basis of morphological features. This is the first record of human infection with the canine whipworm in Japan.

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