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Volume 40, Issue 1
Displaying 1-4 of 4 articles from this issue
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Shinobu ABE, Atsushi YAMAKI, Yutaka DOI, Isao YOSHIOKA1987 Volume 40 Issue 1 Pages 1-13
Published: 1987
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSConcentrated and purified Sabin and virulent strains of poliovirus types 1, 2 and 3 were inactivated with formalin at 37C. By addition of 5.4μM arildone, an antiviral agent, to the virus suspension, the stability of D antigen increased in both Sabin and virulent strains of all types, especially in virulent type 1 Mahoney strain. The drug had neither any inhibitory nor enhancing effect on the formalin inactivation. When antibody response was compared in guinea pigs, Sabin strains inactivated in the absence of arildone were less immunogenic against homotypic virulent strains than inactivated vaccine prepared from virulent strains. On the other hand, Sabin strains inactivated in the presence of arildone were equally immunogenic. These results indicate that it is possible to prepare from Sabin strains a potent and safe inactivated vaccine having an immunogenicity comparable to that prepared from virulent strains.View full abstractDownload PDF (2346K) -
Yoshiyuki MORISHITA, Kimiko SHIROMIZU1987 Volume 40 Issue 1 Pages 15-26
Published: 1987
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSIn the rats fed on lactose-containing purified diet (PDL), bifidobacteria remarkably increased in number in both the jejunum and cecum as compared with those fed on control diet (PD) or conventional diet (NMF) . Besides, lactobacilli, streptococci, staphylococci, and Escherichia coli increased in number in the jejuna and/or ceca of rats fed on PDL as compared with those fed on PD. The incidence and numbers of Lactobacillus species were remarkably different between the rats fed on PDL and those on any of the other diets. Indole-producing Bacteroides spp. were rarely isolated from the PDL-fed rats. The cecum was markedly enlarged in the PDL rats and reduced in the PD rats as compared with that in the NMF animals.View full abstractDownload PDF (1007K) -
Masakazu TAKAHASHI, Masami NISHINA, Eitaro HORI, Kazuhiro MATSUSHITA, ...1987 Volume 40 Issue 1 Pages 27-33
Published: 1987
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESS1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy was used to study aerobic glucose metabolism in eggs of Angiostrongylus cantonensis in an NCTC-109 medium supplemented with fetal calf serum. Without any pretreatment of the spent medium, we were able to identify and quantitate, by NMR, the end-products of glucose metabolism in eggs after cultivation for 2, 4, and 8 days. We demonstrated that A. cantonensis eggs took up glucose rapidly; among the major end products were found lactic acid, acetic acid and alanine. The eggs are parasitic in a sense that the energy metabolism in them is dependent mainly upon the energy source present in outer medium.View full abstractDownload PDF (562K) -
Fumihiko KAWAMOTO, Sahoko MIZUNO, Hisashi FUJIOKA, Nobuo KUMADA, Etsur ...1987 Volume 40 Issue 1 Pages 35-46
Published: 1987
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThree fluorochromes were applied to stain various parasitic protozoans. By double staining with 4', 6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.View full abstractDownload PDF (2486K)
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