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Volume 42, Issue 1
Displaying 1-4 of 4 articles from this issue
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Jean TAYLOR-WIEDEMAN, Kazuyo YAMASHITA, Kikuko MIYAMURA, Shudo YAMAZAK ...1989 Volume 42 Issue 1 Pages 1-11
Published: 1989
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA seroepidemiologic time-comparison study was conducted to evaluate changes in IgG antibody to varicella-zoster virus (VZV) and to determine VZV prevalence in Japan with randomly collected serum samples from two healthy Japanese populations: 1973 (n=670) vs. 1984 (n=677) . Enzyme-linked immunosorbent assay (ELISA) was found to be superior to the immune adherence hemagglutination test (IAHA) especially for detecting seropositivity in adults. Serologic results showed essentially no significant difference between the 1973 and the 1984 age-specific prevalences; with the exception of a slightly lower prevalence in the 5-year-old age group in 1973 than that in 1984. In general, the age-specific prevalence was lowest in the 1-year-old group (mean 11%) and increased in a linear fashion until age 9 (mean 82.9%) ; prevalence continued to increase slowly after age 9 and plateaued at 100% for ages ≥ 25-29.View full abstractDownload PDF (1001K) -
Takuro ENDOH, Kenji YAGITA1989 Volume 42 Issue 1 Pages 13-23
Published: 1989
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA simple method for assessing the infectivity of Toxoplasma gondii using primary cultured monolayer cells has been devised. Statistic analysis important for interpreting the results of such experimentation was made. In this method, the number of intracellular toxoplasmas and the percentage of confluency of the monolayer were measured. The latter value was used for conversion of the number of intracellular toxoplasmas per unit area to the percentage of infective toxoplasmas. A linear dose-response relationship between the number of intracellular toxoplasmas per unit area and that of toxoplasmas inoculated was demonstrated with primary cell monolayers from the lungs of two-day old inbred golden hamsters and the kidneys of newborn mice and newborn Wistar Imamichi strain rats. On the average, 37% of the toxoplasma organisms harvested from the peritoneal exudate of mice on the third or fourth day of infection were found to be infective. This value compares very favorably with the value 40% reported previously.View full abstractDownload PDF (902K) -
Toshiro KUROKI, Shiro YAMAI, Tsutomu KOYAMA1989 Volume 42 Issue 1 Pages 25-29
Published: 1989
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWe report the first human case of Entamoeba polecki infection found in Japan. Cysts of E. histolytica-like amoeba were detected in a stool sample from a female Cambodian refugee. The cysts were morphologically investigated after stained by the modified Kohn's method. The amoeba was identified as E. polecki by the findings that the cyst had frequently an inclusion mass, usually one or very rarely two nuclei, frequently a darkly stained nucleus, and some other characteristics. This protozoan parasite is essentially non-pathogenic to humans, but it is morphologically similar to and often confused with E. histolytica, a pathogenic species. Therefore, it is very important in laboratory diagnosis to differentiate the former from the latter.View full abstractDownload PDF (1759K) -
Nobuo OHTA, Yukio HOSAKA, Masaru MINAI, Masataka HAYASHI, Yasushi MIKI1989 Volume 42 Issue 1 Pages 31-38
Published: 1989
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWe tested effects of praziquantel, an antischistosomal compound, on clinical and immunological parameters of chronic schistosomiasis japonica. Two Japanese patients, who had high antibody titers to Schistosoma japonicum antigens but no fecal schistosome eggs or no or mild symptoms complained, were treated with praziquantel. Within two years after treatment, anti-schistosome antibodies in sera from the patients became negative in enzyme-linked immunosorbent assay. There was no significant alteration in cellular immunity to the parasite. Although S. japonicum infection is believed to have been eradicated in Japan, our present results seem to suggest the possiblity that a few Japanese individuals, who have high anti-schistosome antibody, still harbor live parasites.View full abstractDownload PDF (621K)
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