Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 43, Issue 6
Displaying 1-5 of 5 articles from this issue
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  • GROWTH AND SPREAD OF CAUSATIVE AGENT OF CREUTZFELDT-JAKOB DISEASE IN INTRAPERITONEALLY INFECTED MICE
    Fumitoshi CHINO, Tokitada OHKAWA
    1990 Volume 43 Issue 6 Pages 197-208
    Published: 1990
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.43.197
    JOURNAL FREE ACCESS
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    The progression of Creutzfeldt-Jakob disease (CJD) was studied in ddY mice infected intraperitoneally with the Fukuoka-1 strain, a transmissible agent isolated from a CJD patient in Japan. Infectivity became detectable simultaneously in the brain, the spleen and the liver, i.e. 19 weeks after inoculation. Infectivity titer ranged from 102 to 103 LD50/g in all three organs up to 35 weeks, in contrast to intracerebrally infected mice in which the content of infectious CJD agent is higher in the brain than in the liver and the spleen. Cuffs appeared in the brain roughly four times in the period from 3 to 37 weeks after inoculation. The observed infectivity was discussed in relation to the appearance of cuffs in the brain.
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  • EVALUATION OF ELISA FOR DETECTION OF GIARDIA LAMBLIA-SPECIFIC COPRO-ANTIGEN EMPLOYING MONOSPECIFIC ANTIBODIES
    Parmesh DUTT, Virender Kumar VINAYAK
    1990 Volume 43 Issue 6 Pages 209-217
    Published: 1990
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.43.209
    JOURNAL FREE ACCESS
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    An enzyme linked immunosorbent assay (ELISA) system, using monospecific antibodiesfor the detection of Giardia lamblia specific 66 kDa coproantigen has been developed and evaluated. The assay detected the antigen in stool eluates of all the 24 microscopically confirmed cases of giardiasis and in 17 (68%) of the 25 microscopy-negative clinically suspected cases of giardiasis. None of stool eluates from 20 subjects infected with other protozoal / helminthic intestinal parasites or from 20 apparently healthy subjects had G. lamblia-specific copro-antigen. The ELISA employing monospecific antibodies is a sensitive and specific tool for the diagnosis of giardiasis and is especially useful for confirming microscopy-negative suspected cases of giardiasis.
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  • COMPARATIVE STUDIES ON CLOSTRIDIUM BOTULINUM TYPE A STRAINS ASSOCIATED WITH INFANT BOTULISM IN JAPAN AND IN CALIFORNIA, USA
    Kazue TABITA, Sumiko SAKAGUCHI, Shunji KOZAKI, Genji SAKAGUCHI
    1990 Volume 43 Issue 6 Pages 219-231
    Published: 1990
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.43.219
    JOURNAL FREE ACCESS
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    Twenty strains of Clostridium botulinum type A associated with infant botulism cases, six in Japan and 14 in California, USA, were compared in their characters. All six Japanese strains produced medium-sized progenitor toxin (M toxin; Mr 300 k) but no hemagglutinin and showed lower 50% infective doses (ID50) in the infant mouse test; whereas most American strains produced large-sized progenitor toxin (L + LL toxins; Mr 500 k and 900 k) and hemagglutinin in addition to M toxin and showed higher ID50 in infant mice. No marked difference in the biochemical properties was found between the two groups except for two American strains.
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  • ATTEMPTS TO QUANTITY CLOSTRIDIUM BOTULINUM TYPE A TOXIN AND ANTITOXIN IN SERUM OF TWO CASES OF INFANT BOTULISM IN JAPAN
    Motohide TAKAHASHI, Hiromasa NODA, Seijirou TAKESHITA, Toshiko FUJIWAR ...
    1990 Volume 43 Issue 6 Pages 233-237
    Published: 1990
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.43.233
    JOURNAL FREE ACCESS
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    Serum samples taken from two infant botulism cases during hospitalization were titrated for botulinum toxin by both the intraperitoneal (ip) injection method and the score method in mice. By the ip method, in which death is the only parameter, such low levels of toxin as lower than 4 ip LD50/ml may not be titrated even though the surviving mice show abdominal palsy. By the score method based on the degree of abdominal palsy, such low levels of toxin as 1.1 and 0.8 ip LD50/ml were detected in specimens of one of the patient's serum. No antitoxin was demonstrated in either case of infant botulism by applying the score method. It is not known whether spontaneous recovery from infant botulism is due to the antitoxin production.
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  • SYMPOSIUM ON TOXINS
    1990 Volume 43 Issue 6 Pages 239-280
    Published: 1990
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.43.239
    JOURNAL FREE ACCESS
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