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Volume 44 (1991)
- Issue 5-6 Pages 195-
- Issue 4 Pages 147-
- Issue 3 Pages 87-
- Issue 2 Pages 41-
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Volume 44, Issue 5-6
Displaying 1-5 of 5 articles from this issue
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Eiko KONDO, Pimjai NAIGOWIT, Pensri PHANICHRUTTIWONG, Wimol PETKANCHAN ...1991 Volume 44 Issue 5-6 Pages 195-211
Published: 1991
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSEighty-one fresh isolates of Pseudomonas pseudomallei from melioidosis patients were subjected to the analysis for the fatty acid composition by gas-liquid chromatography (GLC) and pH-dependent pattern of nonspecific phosphatase activity. All the test strains were identical in the GLC profile showing the three peaks of characteristic hydroxy acids (3-OH 14: 0, 2-OH 16: 0, 3-OH 16: 0) and the two prominent peaks of cyclopropane acids (17: 0 Δ, 19: 0 Δ) . They had also basically the same pH-dependent curves of the enzymatic activity with paranitrophenyl phosphate as substrate, showing two to three peaks or shoulders only in the acidic side of the curve. These two biochemical characteristics could differentiate P. pseudomallei distinctly from P. aeruginosa, but not from P. cepacia.View full abstractDownload PDF (1244K) -
Eiko KONDO, Vimol PETKANCHANAPONG, Pimjai NAIGOWIT, Takeshi KURATA, Ko ...1991 Volume 44 Issue 5-6 Pages 213-224
Published: 1991
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSPseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.View full abstractDownload PDF (3398K) -
Koomi KANAI, Eiko KONDO1991 Volume 44 Issue 5-6 Pages 225-237
Published: 1991
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe substrate response in acid phosphatase activity of Pseudomonas pseudomallei and Pseudomonas cepacia was examined with different phosphate esters including hexose phosphates and phosphoaminoacids in a whole cell assay system. The enzymatic activity against each substrate was evaluated in terms of percent activity to that against para-nitrophenyl phosphate set as 100. A remarkable finding was that the phosphatase reaction was the highest with phosphotyrosine or phosphoserine as substrate showing 180% activity. This tyrosine phosphatase activity was resistant to heating at 60 C for 20 min and inhibited greatly by 0.1% ZnCl2. Pseudomonas cepacia showed the same pattern of substrate response and the same characteristics of tyrosine phosphatase activity.View full abstractDownload PDF (930K) -
Seema SOMJI, Shahana U. KAZMI, Azra SULTANA1991 Volume 44 Issue 5-6 Pages 239-243
Published: 1991
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSAn epidemiological study was carried out to determine the prevalence of Chlamydia infections in adult females by enzyme immunoassay and microscopic examination of Giemsa-stained smears. Endocervical swabs were collected from 126 females attending OB/GYN ward at Abbasi Shaheed Hospital, Karachi. 13.5% of 126 females tested were positive by enzyme immunoassay and only 5.6% were positive by the Giemsa-staining method. The infection rate among pregnant and nonpregnant women with urinogenital problems were 11.8% and 14.7%, respectively. The majority of females complained of excessive cervical discharge and pain in the lower abdomen. A high prevalence of infection in normal pregnant women (18.2%) indicates the asymptomatic nature of this infection.View full abstractDownload PDF (394K) -
1991 Volume 44 Issue 5-6 Pages 245-272
Published: 1991
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (2336K)
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