巻号一覧
44 巻 (1991)
- 5-6 号 p. 195-
- 4 号 p. 147-
- 3 号 p. 87-
- 2 号 p. 41-
- 1 号 p. 1-
- Supplement 号 p. 9-
44 巻, 5-6 号
選択された号の論文の5件中1～5を表示しています
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Eiko KONDO, Pimjai NAIGOWIT, Pensri PHANICHRUTTIWONG, Wimol PETKANCHAN ...1991 年 44 巻 5-6 号 p. 195-211
発行日: 1991年
公開日: 2010/03/19
ジャーナル フリーEighty-one fresh isolates of Pseudomonas pseudomallei from melioidosis patients were subjected to the analysis for the fatty acid composition by gas-liquid chromatography (GLC) and pH-dependent pattern of nonspecific phosphatase activity. All the test strains were identical in the GLC profile showing the three peaks of characteristic hydroxy acids (3-OH 14: 0, 2-OH 16: 0, 3-OH 16: 0) and the two prominent peaks of cyclopropane acids (17: 0 Δ, 19: 0 Δ) . They had also basically the same pH-dependent curves of the enzymatic activity with paranitrophenyl phosphate as substrate, showing two to three peaks or shoulders only in the acidic side of the curve. These two biochemical characteristics could differentiate P. pseudomallei distinctly from P. aeruginosa, but not from P. cepacia.抄録全体を表示PDF形式でダウンロード (1244K) -
Eiko KONDO, Vimol PETKANCHANAPONG, Pimjai NAIGOWIT, Takeshi KURATA, Ko ...1991 年 44 巻 5-6 号 p. 213-224
発行日: 1991年
公開日: 2010/03/19
ジャーナル フリーPseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.抄録全体を表示PDF形式でダウンロード (3398K) -
Koomi KANAI, Eiko KONDO1991 年 44 巻 5-6 号 p. 225-237
発行日: 1991年
公開日: 2010/03/19
ジャーナル フリーThe substrate response in acid phosphatase activity of Pseudomonas pseudomallei and Pseudomonas cepacia was examined with different phosphate esters including hexose phosphates and phosphoaminoacids in a whole cell assay system. The enzymatic activity against each substrate was evaluated in terms of percent activity to that against para-nitrophenyl phosphate set as 100. A remarkable finding was that the phosphatase reaction was the highest with phosphotyrosine or phosphoserine as substrate showing 180% activity. This tyrosine phosphatase activity was resistant to heating at 60 C for 20 min and inhibited greatly by 0.1% ZnCl2. Pseudomonas cepacia showed the same pattern of substrate response and the same characteristics of tyrosine phosphatase activity.抄録全体を表示PDF形式でダウンロード (930K) -
Seema SOMJI, Shahana U. KAZMI, Azra SULTANA1991 年 44 巻 5-6 号 p. 239-243
発行日: 1991年
公開日: 2010/03/19
ジャーナル フリーAn epidemiological study was carried out to determine the prevalence of Chlamydia infections in adult females by enzyme immunoassay and microscopic examination of Giemsa-stained smears. Endocervical swabs were collected from 126 females attending OB/GYN ward at Abbasi Shaheed Hospital, Karachi. 13.5% of 126 females tested were positive by enzyme immunoassay and only 5.6% were positive by the Giemsa-staining method. The infection rate among pregnant and nonpregnant women with urinogenital problems were 11.8% and 14.7%, respectively. The majority of females complained of excessive cervical discharge and pain in the lower abdomen. A high prevalence of infection in normal pregnant women (18.2%) indicates the asymptomatic nature of this infection.抄録全体を表示PDF形式でダウンロード (394K) -
1991 年 44 巻 5-6 号 p. 245-272
発行日: 1991年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (2336K)
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