巻号一覧
45 巻, 3 号
選択された号の論文の6件中1～6を表示しています
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Ashim GHATAK, Giovanni MONTE, Martha GARCIA, 都島 基夫1992 年 45 巻 3 号 p. 99-111
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (1393K) -
渡理 英二, 横室 公三1992 年 45 巻 3 号 p. 113-125
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーEight T-cell hybridomas were established from the draining lymph node of C3H mice immunized with Semliki forest virus (SFV) . Six of them showed specificity toward viral-structure protein E2, while the remaining two clones included one with specificity to an other structural protein E1 and the other with specificity to C. The production of IL-2 by the E2 protein-specific T-cell hybridomas in the presence of SFV was suppressed by treating the antigenpresenting cells (APC) with ammonium chloride raising pH of the acidic compartments. It was found also that treatment of APC with a thiol protease inhibitor, leupeptin or E64, resulted in a reduced response of some of the E2-specific T-cell hybridomas. The E2 protein of SFV proved to be resistant at pH7.0, and sensitive at pH5.0 to in vitro cathepsin B treatment. In contrast, the E1 and C proteins proved to be resistant to both pH values. These results indicate that the thiol protease, probably cathepsin B, works as one of the enzymes group involved in antigen processing.抄録全体を表示PDF形式でダウンロード (2525K) -
Hong-Guang GUO, Kenneth Shueh-Shen CHANG1992 年 45 巻 3 号 p. 127-136
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーWe used polymerase chain reaction (PCR) to amplify a segment, about 560 base pairs (bp), of HIV-1 gag DNA prepared from peripheral blood mononuclear cells of a seropositive Taiwanese pair of mother and infant. TM-1 and TC-1 clones of PCR-amplified DNA derived from the mother and infant, respectively, showed a 94.5% homology with each other. However, the TM-1 and TC-1 sequences exhibited lower degrees of homology, i.e. only 85.1% and 85.8%, respectively, with the corresponding gag segment of a North American HIV-1 subtype (HXB2), and 86.4% and 87.0%, respectively, with that of a Zairean HIV-1 subtype (Z2Z6) . The divergence of TM-1 and TC-1 sequences from those of HXB2 and Z2Z6 is particularly prominent in the first (5' proximal) 200 bp of the cloned DNA segment, involving transitions more frequently than transversions. Two additional clones TM-2 and TC-2 derived from the mother and infant were sequenced for the first 200 bp. These four clones showed a high degree of homology (94.7-97.5%) among themselves, providing an evidence for transmission of the virus from the mother to the infant. These findings show the epidemiological value of PCR, and indicate the presence of a gag subtype of HIV-1 which is distinct from both the North American and Zairean subtypes according to the phylogenetic tree constructed.抄録全体を表示PDF形式でダウンロード (879K) -
Mohammed Jameela BANU, Kaliappan NELLAIAPPAN, Subramanian DHANDAYUTHAP ...1992 年 45 巻 3 号 p. 137-150
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーMitochondrial malate dehydrogenase (mMDH) and malic enzyme (mME) of a filarial worm Setaria digitata were studied. mMDH exhibited the highest activities in the oxidation and reduction reactions at pH 9.5 and pH 6.2, respectively, while mME did so in the malate decarboxylation reaction at pH 6.8. mME showed no detectable activity on the pyruvate carboxylation direction. The Km values for malate (1.7 mM) and oxaloacetate (0.17 mM) and the ratio of Vmax oxidation: Vmax reduction (2.73) tend to favor the oxaloacetate reduction by mMDH, mME showed a relatively high Km value of 8.3 mM, for malate decarboxylation. A drug, diethylcarbamazine citrate (DEC-C), did not change appreciably the activity of either mMDH or mME, while filarin (a drug of herbal origin) effectively inhibited mMDH. The leaf extracts of Ocimum sanctum, Lawsonia inermis and Calotropis gigantea and leaf and flower extracts of Azadirachta indica were, however, found to inhibit both mMDH and mME.抄録全体を表示PDF形式でダウンロード (1035K) -
山下 和予, 宮村 紀久子, 山寺 静子, 加藤 信子, 赤塚 昌江, 井上 栄, 山崎 修道1992 年 45 巻 3 号 p. 151-161
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーIn Japan, aseptic meningitis cases due to enterovirus infections increase every summer in various degrees with an incidence peak usually in July. During the past 11 years from 1981 through 1991, a total of 8, 595 enterovirus isolations from aseptic meningitis cases were reported from 54 participating laboratories. Eight enterovirus types caused large epidemics; more than 100 isolations of each type from aseptic meningitis cases were reported for every epidemic year of the respective type. They were coxsackievirus (C) types B3 and B5, echovirus (E) types 4, 6, 7, 9, 18 and 30. Among these, the highest meningitisassociating frequency was reported for E30, representing 82.6% of the total isolations reported for the type during this period, followed by E4, 71.1%. The frequencies of E9, E7, E6 and CB5 were in a range from 54.5% to 44.4%, while that of E18 was 37.7% and that of CB3 21.0%. During the epidemics, enterovirusassociated meningitis was most frequently reported among children of 4-7 years of age. High frequencies were also shown in infants less than 1-year of age in some types. A total of 4, 240 enteroviruses were isolated from cerebrospinal fluid of aseptic meningitis cases, representing 49.3% of the cases with enterovirus isolation.抄録全体を表示PDF形式でダウンロード (874K) -
1992 年 45 巻 3 号 p. 163
発行日: 1992年
公開日: 2010/03/19
ジャーナル フリーPDF形式でダウンロード (54K)
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