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Volume 49, Issue 3
Displaying 1-5 of 5 articles from this issue
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Ren-Ku TSENG, Hour-Young CHEN, Chi-Byi HONG1996 Volume 49 Issue 3 Pages 77-93
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSIn order to understand the epidemic trends of influenza virus infection in Taiwan, 5, 882 throat-swab specimens were collected from June 1979 to June 1995. Influenza virus was detected in 313 specimens including samples collected at Taichung and Tainan from 1981 to 1982. Among them, 214 isolates (68.4%) were identified as influenza virus type A, and 99 (31.6%) as type B. In the course of the suveillance, the influenza virus strain A/Taiwan 1/86 (H1N1), known as a world-wide reference strain, was isolated in April 1986. Influenza virus infection was identified throughout the year in Taiwan based on the frequency of detection of the virus. Some strains were referred to as intermediate strains in comparison with the reference strains on the basis of antigenic heterogeneity. About 80% of the isolates identified in this laboratory were from children under 12 years old. The rate of isolation of virus was about 46% during the epidemic season.View full abstractDownload PDF (1132K) -
Akter PARVIS, Rahman MAJIBUR, Bhuiyan SHAKHAWAT, Rahman ARIFUR, Hossai ...1996 Volume 49 Issue 3 Pages 95-102
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThis work focuses mainly on the effect of NaCl along with other mono and divalent anions and rations at pH 4 on survival and virulence properties of Aeromonas hydrophila. To find the optimum stress condition, effects of several other physical factors on NaCl induction were also assayed. A. hydrophila collected from International Center for Diarrheal Disease Research, Bangladesh (ICDDR, B) was found to be more sensitive to pH 4.0 when grown in media containing 200 mM or more salt. Induction of acid sensitivity was rapidly gained in NaCl-supplemented broth at 37 C. There was only slight sensitization after 5 min, but a marked effect was observed within 30 min. Uninduced A. hydrophila gave an average of 40% survival after exposure to pH 4.0 for 5 min. However, NaCl-induced cells of this organism reduced the survival to a great extent having an average of 0.06%. Either increase in salt concentration (100 mM through 400 mM) or decrease (50 mM) resulted in decrease in percent survival when exposed to gradually increased concentrations of salt. The most effective dose for induction was 400 mM NaCl salt. NaCl-induction was found to be more pronounced in log-phase organisms. All other monovalent and divalent anions and cations tested were almost as effective as NaCl except KCl.View full abstractDownload PDF (620K) -
Tomoko KOBAYASHI, Hiro-omi TAMURA, Ryo TAGUCHI, Shigezo UDAKA, Hiroh I ...1996 Volume 49 Issue 3 Pages 103-112
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWe succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.View full abstractDownload PDF (2266K) -
Masahiro FUJITA, Takahisa FURUTA, Somei KOJIMA, Takeshi KURATA, Yasuhi ...1996 Volume 49 Issue 3 Pages 113-120
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA serosurvey for Pneumocystis carinii infection of laboratory-bred and wild-born monkeys was made by the indirect immunofluorescence method. The antibody titers of wild cynomolgus (Macaca fascicularis) and Japanese monkeys (Macaca fuscata fuscata) to P. carinii (Pc) were higher than those of laboratory-bred cynomolgus monkeys; 54.9% of the wild cynomolgus and 40% of Japanese monkeys had antibody titers higher than 1: 40, although only 9.4% of the laboratory-bred cynomolgus monkeys did so. Pc cysts in the lungs were examined in the laboratory-bred cynomolgus monkeys by using toluidine blue O stain, and the number of cysts in the lung was small, when compared with those in the lungs of SIV-infected monkeys showing severe pneumosystosis. The results obtained from the studies suggested the possibility that P. carinii infection was more widely epidemic in the wild-born monkeys than in laboratory-bred monkeys.View full abstractDownload PDF (655K) -
Kazuhiko MEIGATA, Ryo HONDO, Akikazu FUJIMA, Mika SHINKAI-SHIBATA, Sei ...1996 Volume 49 Issue 3 Pages 121-127
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSWe titrated human cytomegalovirus (HCMV) DNA in urine specimens obtained from 14 healthy individuals and a renal transplant patient with HCMV pneumonitis by modifying the method for titration of varicella-zoster virus DNA previously described (1, 2) . Of 14 HCMV seropositive healthy individuals, 13 had HCMV DNA under the detection limit of 102.0 copies/ml, whereas one person had 102.0 copies/ml. The viral DNA in urine samples was at a low level in healthy individuals with latent infection. In a case with HCMV pneumonitis after renal transplantation, the amount of HCMV DNA in urine gradually in creased from the level under 102.0 copies/ml and reached a peak of 104.7 copies/ml one month prior to the manifestation of pneumonitis. It, thereafter, decreased with the course of clinical remission, and finally settled at under 102.0 copies/ml. Serial titrations of HCMV DNA in urine specimens proved to be useful in identifying recipients at risk of developing active HCMV infection after renal transplantation and as a guide for treatment of patients.View full abstractDownload PDF (1915K)
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