All issues
Successor
Predecessor
Volume 49, Issue 4
Displaying 1-4 of 4 articles from this issue
- |<
- <
- 1
- >
- >|
-
S. Chia-Tung PAN1996 Volume 49 Issue 4 Pages 129-149
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (22120K) -
Ramasamy SELVAM, Govindarajan BASKARAN1996 Volume 49 Issue 4 Pages 151-165
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe hematological parameters were assayed in Plasmodium vivax patients with only one infection, two infections, three infections and more than three malarial infections during a period of six months. A steady fall in the levels of hemoglobin as well as packed cell volume (PCV) level was observed with increasing number of infections. The malarial patients showed a progressive decrease in RBC level with increasing number of attacks. The decrease in the hematological indices was statistically significant at all levels of parasitemia. There was a marked increase in the osmotic fragility of the malarial erythrocytes when compared to that of controls. During repeated malarial attacks, significant decrease in MCH (p<0.05) and MCHC (p<0.01) and increase in the MCV level (p <0.05) and Heinz body formation (p<0.001) were observed. Parasite density significantly influenced the fragility of the erythrocytes, Heinz body formation, MCV, MCH and MCHC levels. Thus, the erythrocytes of the patients repeatedly infected with Plasmodium vivax parasite are subjected to structural and functional impairment, ultimately culminating in anemia.View full abstractDownload PDF (1123K) -
Junko AMEMURA-MAEKAWA, Fumiaki KURA, Haruo WATANABE1996 Volume 49 Issue 4 Pages 167-186
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA facultative intracellular parasite Legionella pneumophila has two kinds of superoxide dismutase (SOD), iron-containing superoxide dismutase (Fe-SOD) and copper, zinc-containing one (Cu, Zn-SOD) . We cloned both SOD genes of L. pneumophila and determined their DNA sequences. The Fe-SOD gene (sodB), isolated by functional complementation of a SOD-deficient Escherichia coli strain, encoded a protein of 192 amino acids conserving the Fe-SOD-specific amino acid residues. A clone containing entire Cu, Zn-SOD gene (sodC) was constructed by connecting two contiguous DNA fragments; one with a lower part of the gene was obtained by colony hybridization with a probe acquired by polymerase chain reaction (PCR) with degenerate oligonucleotide primers corresponding to conserved regions of known Cu, Zn-SOD genes and the other with an upper part of the gene was by IPCR (inverted PCR) . The sodC gene encoded a protein of 162 amino acids, of which the first 20 amino acids inferred a signal peptide similar to other bacterial Cu, Zn-SODs reported previously. Both clones expressed their SOD activities in E. coli K-12 through their own plausible promoters. We examined for SOD genes on chromosomes of several Legionella species. All chromosomes were hybridized with Fe-SOD gene of L. pneumophila, but Cu, Zn-SOD gene did not hybridize to the chromosomes of other than L. pneumophila strains.View full abstractDownload PDF (6051K) -
Sadanori SHINTAKU, Yasuhiko FUKUDA, Akinori KIMURA, Shunji HOSHINO, To ...1996 Volume 49 Issue 4 Pages 187-200
Published: 1996
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSSixty-five haplo-identical living-related renal transplant pairs were subjected to comparative studies for HLA class-I typing suitability between the DNA and serological methods. Our HLA-A genotyping method was highly resolutive and allowed assigning 33 serologically blank specificities and subdividing some HLA-A serological specificities, of which A2 discrimination was considered to be indispensable for the matching analysis because of its high frequency among Japanese. Our HLA-B genotyping method made it possible to identify the 17 serologically blank specificities despite their“low resolutive”capacities. Analysis of 14 DRB1-compatible pairs suggested that HLA-A and -B compatibilities had beneficial effects on the long term graft survival. It was concluded that HLA-A and -B should be genotyped for the matching analysis of the kidney transplant pairs to obtain satisfactory graft outcome.View full abstractDownload PDF (1264K)
- |<
- <
- 1
- >
- >|