Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
50 巻, 3 号
選択された号の論文の4件中1~4を表示しています
  • Kaushal KUMAR, Syed Jamil-Ur RAHMAN, Sushil Kumar SHARMA, Kuldip Singh ...
    1997 年 50 巻 3 号 p. 97-111
    発行日: 1997年
    公開日: 2010/03/19
    ジャーナル フリー
    Studies carried out in the states of Maharashtra, Gujarat, Uttar Pradesh and Union Territory of Delhi after the bubonic plague outbreak during 1994 revealed the presence of seven species of rodents, viz.Rattus rattus, R. norvegicus, Mus. musculus, Tat era indica, Suncus murinus, Bandicoota bengalensisandB. indica. The flea species encountered wereXenopsylla cheopisandX. astia. TheX. cheopisandX. astiaindex recorded in different areas of Beed district of Maharashtra; Surat, Vadodra and Baruch districts in Gujarat and Varanasi district in Uttar Pradesh and their implications have been discussed. Insecticide suspectibility tests carried out against DDT, dieldrin, malathion and deltamethrin withX. cheopiscollected from Maharashtra, Delhi and Varanasi revealed that this vector species is resistant to DDT and dieldrin but susceptible to malathion and deltamethrin. The prevalence and distribution of rodents species, highcheopisindex and prevalence ofTatera indicajust at the door steps of houses in village Mamla of Beed district provides highly congenital conditions for the intermingling of wild and domestic rodents and transfer of flea population from wild to domestic rodents and vice-versa. These conditions were found to be highly supportive for bubonic plague transmission in the district. The presence ofYersinia pestisantibodies inRattus rattuscollected from Beed, Surat and Varanasi areas are also indicative of bubonic plague in Beed and Varanasi and pneumonic plague in Surat during 1994.
  • Dong Wook RYANG, Sang Won CHO, Myung Geun SHIN, Jong Hee SHIN, Soon Pa ...
    1997 年 50 巻 3 号 p. 113-121
    発行日: 1997年
    公開日: 2010/03/19
    ジャーナル フリー
    This study was undertaken to determine molecular types and genetic similarity amongV. vulnificusisolates by RAPD analysis. We compared these results with serotypes ofV. vulnificus. Ninety-sevenV. vulnificusstrains including 69 strains from Chonnam University Hospital (CUH; Kwangju, Korea), 13 from Wonkwang University Hospital (WUH; Iksan, Korea), 13 from the Japanese National Institute of Health (JNIH) and two reference strains (ATCC 33815 and ATCC 27562) were analyzed. Four molecular types comprising all the strains were obtained by RAPD analysis. Type I was the most common (60/95) and included 58 strains from CUH. Type I showed a further subdivision into seven subtypes. Type II (23/95) composed of 11 strains from CUH, nine from WUH, three from JNIH and two reference strains. Six type III strains comprised four WUH strains and two JNIH strains. All six strains of type IV were from JNIH. The range of genetic similarity values amongV. vulnificusisolates was 0.24 to 1.00. The serotypes of 95 strains were O4 (84.2%), O14 (3.2%), O1 (2.1%), O13 (2.1%), and R (2.1%) . The most common O4 serotype strains were distributed among types I (60 strains), II (23 strains), III and IV (six strains) . Although theV. vulnificusisolates showed a wide range of genetic similarity values, RAPD analysis could separateV. vulnificusstrains into four molecular types, and the isolates from the same hospitals tended to belong to the same molecular type. There was no specific correlation between molecular types and serotypes ofV. vulnificus.
  • 清原 知子, 佐藤 知子, 山本 弘史, 戸塚 敦子, 森次 保雄
    1997 年 50 巻 3 号 p. 123-131
    発行日: 1997年
    公開日: 2010/03/19
    ジャーナル フリー
    Age-specific prevalence of anti-hepatitis A virus antibody (antiHAV) was surveyed with 2, 708 sera collected in 1994 in various areas of Japan. By age-group analyses, we found strong association of anti-HAV antibody with higher. age group. The prevalence ratios of antibody in the groups of 30-34, 35-39, 40-44, 45-49, 50-54, 55-59, 60-64 and 65 years or older were 0, 4.2, 22.0, 44.8, 57.6, 76.4, 84.5 and 91.4%, respectively. Geometric mean titers of anti-HAV antibody in the positive age groups were approximately 6, 000 mIU/ml. The seropositives among older population were ascribed to the infections more than 40 years ago and the high anti-HAV titers have been maintained since that time. In Japan, people younger than 40 years of age are extremely risky to HAV infection, since 99% have no antibody. Those in forties are also risky since two-thirds of them are seronegative. In Japan, an inactivated vaccine was licensed in 1994. Vaccination may be recommended for such high-risk groups as travelers going to endemic areas, patients who have received blood product medication and child-care staffs.
  • 川津 健太郎, 濱野 米一, 依田 知子, 寺野 由剛, 柴田 忠良
    1997 年 50 巻 3 号 p. 133-150
    発行日: 1997年
    公開日: 2010/03/19
    ジャーナル フリー
    A monoclonal antibody against tetrodotoxin (TTX) was obtained from Balb/c mice immunized with TTX-bovine serum albumin (BSA) conjugate. The monoclonal antibody was highly specific for TTX and had no cross-reaction to tetrodonic acid, which is a TTX derivative, or gonyautoxins, although a minor cross-reaction to anhydro-tetrodotoxin was observed. The monoclonal antibody neutralized the lethal activity of TTX. By using the monoclonal antibody, a rapid and highly sensitive competitive enzyme immunoassay (EIA) for quantitative analysis of TTX was developed. By the competitive EIA system, TTX can be determined quantitatively in about 30 min (90 min are required if the time for preparation of the solid-phase antigen was included), and the working range for quantitative analysis of TTX was 2-100 ng/ml. In recovery tests and examinations of TTX samples, results of the mouse bioassay and ETA analyses correlated well (r=0.987) . Moreover, it was demonstrated that low concentrations of TTX, which could not be detected by the mouse bioassay, could be determined quantitatively by the competitive EIA.
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