As is well known, the number of eggs produced by a mosquito varies extensively, depending on the kind or amount of the ingested blood. Some important results of the recent investigations on this subject are summarized in the book by Bates (1949) . Previous information may be classified in two categories, one of which is dealing with utility of blood or blood fractions on egg development and the other with physiological differences of the mosquitoes, such as autogeny, diapause, gonotrophic dissociation, etc. The two problems are to be intimately related to each other; nevertheless there has been little work made on elucidating, for instance, the diapause state of adult females in terms of metabolic processes in the ovarian follicles.
The attempt, however, will not be easily realized at once, especially with such species as the Japanese Culex pipiens pallens in which no fundamental knowledge of the egg production has yet been obtained. Hence the present work was undertaken, to begin with, for ascertaining the fate of ovarian follicles during the course of their development. Techniques and methods are essentially not different from those described before (Hosoi, 1953) . In case statistical treatments are required, the significance of the differences is judged, or the confidence limits of the means are taken, by a 95% confidence coefficient.

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It has been pointed out in Part III of this report that immense variations appear in the number of matured follicles when mosquitoes of equal wing length are fed to repletion on human blood (Hosoi, 1953b) . Since growing follicles decrease in number usually by a fairly constant ratio, the above fact may presumably be due to differences in the number of follicles contained in the mosquitoes at the time of emergence. To what extent does this number relate to the wing length, or in what way is the relation changed by the breeding conditions? The following experiments were carried out for analysis of the problem.

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In the 1st and 2nd reports on a series of these studies, the authors discussed about a “phenomenon” of the so-called “Crystal Semi-permeable Membrane” by Miyamoto and Takigawa. They concluded that such a phenomenon can be observed, not peculiarly in silicate, but generally in all cases when water-insoluble salts are formed at the “Pringsheim's equilibrium point”, and they built up a conception of the crystal semi-permeable membrane.
By the way, the most parts of the semi-permeable membrane are made of organic high-molecular compounds ; the inorganic one has not seemingly been found since the membrane of copper ferrocyanide reported by Pfeffer in 1877. Even in the Pfeffer's membrane, there is nothing reliable on the “conditions and mechanism” of its formation, while natures after its formation have been considerably studied and cleared up.
A series of the following experiments were carried out with it into consideration that the Pfeffer's semi-permeable membrane will belong to the category of “crystal semi-permeable membrane” made by the present authors; that is, there is anyhow an equilibrium point in forming the so-called Pfeffers semi-permeable membrane from copper sulfate and potassium ferrocyanate, which Pfeffer is thought to have accidentally discovered.

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Burnet and Stone (1) reported that vaccinia hemagglutinin, which is to be referred to as VH, is a soluble substance which is distinct from the elementary body, as shown by suitable adsorption experiments. However, they could not show the elution of VH after prolonged contact with susceptible fowl erythrocytes. They suggested that VH is complexes of a phospholipid (probably lecithin) with an antigenically specific virus constituent, because VH was destroyed by phospholipid-splitting enzymes, such as C1. welchii type A toxin or cobra venom. Since protamine and histone were found capable of inhibiting vaccinia hemagglutination, Fisher (2) suggested that the red cell receptors for the phospholipid might be protein in nature. On the basis of some physicochemical and immunological analysis. Chu (3) (4) concluded that VH has thermolabile and thermostable components, which are distinct from L, S antigen. Gillen and his collaborators (5), and Briody (6) have shown that the thermostable and thermolabile components are of different particle size. Gillen and his collaborators (5) pointed out that the heat stable VH is more closely associated with the elementary body and not Seitz-filtrable and can be sedimented by centrifugation at 17, 000 rpm in three hours. They also showed that the soluble heat labile hemagglutinin is completely separable from the inf ective virus units. Briody (6), however, advocated a “unitarian” view of VH, suggesting that VH has a range of weights. Using fresh serum of guinea pig, Briody and his collaborators (7) demonstrated that the egg infectivity of vaccinia virus is neutralized, while VH remained unaffected. Thus, many important properties of VH have been found by these authors, but more detailed characteristics of VH are wanted to be established. The present paper deals with the results of investigation into the influence of some physico-chemical procedures upon VH.

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In a previous paper (1953) of this series was studied the perpendicular distribution and rheotaxis of the miracidium of Fasciola hepatica in water. As well known, the miracidium of F. hepatica showed a marked positive phototaxis. Takahashi (1927), Suzuki (1931) and others stated that the major part of the miracidia of this species generally advanced toward the light. However, these previous observations were all made fragmentarily in the course of other studies and there is no experimental study on the behavior of the miracidium of this trematode to light. The present paper is a record of various experimental study of phototaxis of the miracidium of F. hepatica.

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It was confirmed in our previous reports on the amount of free aminoacids within bacterial cells of sulphathiazol resistant strain of dysentery bacilli and of streptomycin resistant strain of Staphylococcus aureus that more aspartic acid is found in cells when cultivated in the presence of the drugs, to which they are resistant, than when cultivated in their absence. However, when streptomycin resistant strain of BCG was tested along the same line, somewhat different observations were obtained as will be described in the present paper.

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Since the first report of Bray in 1945 on Bacterium coli neapolitanum, which was antigenically identified as Escherichia coli O-111 B4 by Kauffmann in 1950 and was suspected to be the cause of summer diarrhea of infants, numerous papers on this subject have been published. Ferguson performed the feeding experiments with this organisms on 114 adult volunteers and concluded that symptoms similar to acute bacterial food-poisoning developed when the dosage of organisms was large. It was suggested that normal adults are resistant to infection under normal conditions but that Escherichia coli O-111 B4 may cause diarrhea in the debilitated adult. In Japan, Fukumi and Kosakai carried out the feeding experiments on adult volunteers with Escherichia coli O-111 B4 and non type-specific strains of coliform organisms isolated from normal human adult and monkey. Their results were almost the same as those of Ferguson, except that mild diarrhea occurred when the volunteers were fed with the monkey strain.
The intubation study with Miller-Abbott's double lumen tube in feeding adult volunteers with Escherichia coli O-111 B4, to be reported in this paper, were designed with the following purposes: (1) to determine the site of multiplication of these organisms in the intestinal tract in the living state ; (2) to discuss the relationship between the multiplication of these organisms and the manifestations of symptoms of so called “Coli Enteritis”.

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