Nihon Yoton Gakkaishi
Online ISSN : 1881-655X
Print ISSN : 0913-882X
ISSN-L : 0913-882X
Volume 44, Issue 2
Displaying 1-4 of 4 articles from this issue
Original
  • Satoru IJIRI, Aki NAKAYAMA, Kimitaka NAKANO, Shinya YAMAUCHI, Koji KAK ...
    2007 Volume 44 Issue 2 Pages 31-39
    Published: June 20, 2007
    Released on J-STAGE: December 05, 2007
    JOURNAL FREE ACCESS
    Fermented liquid feed (FLF), which was designed for growing-finishing pigs based on nutritional requirements, was prepared using by-products of food processing such as bread crusts, potato peelings, potato chip crumbs, jam residue, green-tea sullage, bean curd refuse, and other ingredients. FLF was fermented for 20hr at 33°C using Lactobacillus plantarum A305 as a starter. After fermentation, the concentration of lactic acid bacteria in the FLF reached 109 cfu/g and the pH value of the FLF decreased to 4.2.
    A feeding experiment was carried out to examine the difference in growth effects between formula feed (FF) and FLF. In the experiment, 11 pigs were used. They were divided into two groups based on weight. A control group was fed FF and an experimental group was fed FLF, ad libitum for 16 days. The average daily weight gain (DG) for the control group and the experimental group was 965g/d and 1,207g/d respectively (P<0.05). The growth performance of pigs fed FLF increased in proportion to nutritional intake, and the feed conversion ratio calculated based on the total dry matter intake of the experimental group fed FLF (2.96) was superior to that of the control group fed FF (3.16) in the experiment.
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  • Koji KAKUGAWA, Satoru IJIRI, Kimitaka NAKANO, Shinya YAMAUCHI, Yoshino ...
    2007 Volume 44 Issue 2 Pages 40-50
    Published: June 20, 2007
    Released on J-STAGE: December 05, 2007
    JOURNAL FREE ACCESS
    A fecal microbial community structure in pigs fed fermented liquid feed was investigated. The phylogenetic diversity of the fecal microbial community in pigs was analyzed by the PCR-clone library study and PCR-DGGE analysis. The V3 region of 16s rDNA was amplified by PCR using total DNA extracted from pig feces as a template. A clone library comprising of 120 cloned 16s rDNA sequences was classified into 39 ribotypes by RFLP analysis, and all clones were found to belong to Bacteroides, Spirochaetes, and Firmicutes. The abundance ratio of Firmicutes increased greatly from 66.7% to 83.3% for a pig fed fermented liquid feed. From the results of PCR-DGGE, Pig26 clone that belongs to Ficmicutes showed a marked increase. Because the homology between 16s rDNA of a known bacterium and Pig26 clone was low (<86%) as analyzed by Blast, we could not identify the Pig26 clone. Therefore, it was impossible to presume a feeding effect of fermented liquid feed based on a dominant organism name. On the other hand, the gastric acid resistance of Lactobacillus plantarum A305 used as a starter was insufficient. Therefore, Lactobacillus plantarum A305 was hardly able to grow in the digestive organ. Taken all together, the change in a pig's microbial community due to Lactobacillus plantarum A305 was slight and that change did not influence the pig's health.
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  • Shinya YAMAUCHI, Koji KAKUGAWA, Hideyuki MATSUMOTO, Yoshinubu TSUCHIYA ...
    2007 Volume 44 Issue 2 Pages 51-58
    Published: June 20, 2007
    Released on J-STAGE: December 05, 2007
    JOURNAL FREE ACCESS
    A conversion method for liquid feed for pigs by lactic acid fermentation was investigated for the purpose of recycling food by-products generated from food companies in Hiroshima prefecture. Batch cultivations were performed to obtain the growth properties such as specific growth rate (μ), growth yield from glucose (Yx/s) and lactic acid yield from glucose (Yp/s) at 30 or 38°C, using three types of lactic acid bacterium. Moreover, the possibilities of utilization of starch and stomach acid tolerance and bile tolerance of lactic acid bacteria were also examined.
    From the results, Lactobacillus plantarum A305 was shown to have the highest aptitude. A305 had both high growth rates and greater possibilities for utilizing starch compared with the other two. Furthermore, A305 had good growth rates as both a rice-cleaning drainage culture medium used as a pre starter and as a food by-products culture medium of the same composition as liquid feed.
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  • Naoko OIKAWA-TAKADA, Toshio OSHIDA, Naoko MORI, Chikaku DOHGASAKI
    2007 Volume 44 Issue 2 Pages 59-65
    Published: June 20, 2007
    Released on J-STAGE: December 05, 2007
    JOURNAL FREE ACCESS
    The concentrations of vitamin E (hereafter, V.E) in sow's milk were measured from parturition to weaning. Changes in these V.E concentrations, the correlation between V.E concentrations in the colostrum and those in sow's plasma and erythrocytes, and further correlations between V.E concentrations and selenium concentrations in colostrums were examined. The highest value (17.8μg/ml) of V.E concentration was obtained in the colostrum at parturition. V.E concentrations maintained high levels (average 14.9μg/ml) during the four days after parturition, and then declined to the lowest level (3.1μg/ml) at weaning (21 days after parturition). The V.E concentrations 7 days before parturition were 4.05μg/ml in the plasma and 0.47μg/ml in the erythrocytes. Significant correlations were observed between colostrum V.E concentrations and plasma V.E concentrations (r=0.500, p<0.05) and also between V.E concentrations and selenium concentrations in milk (r=0.649, p<0.01).
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