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  • 標題: tiselius OR
  • 標題: 移動界面電気 OR
  • 抄録: 移動界面電気 OR
  • 抄録: tiselius

検索結果 34件

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電気泳動法のトリロジー
1-4
「電気泳動法のトリロジー」と題して電気泳動法の歴史の一端を述べる.1915年『化学本論』に「粒子が動電力に従い動く.この種の現象を電気泳動と云う.」と記されているが,1807年ロシアのReussが電気泳動と電気浸透の現象を報告したとされている.その後,スウェーデンのTiseliusが,移動境界電気泳動法を開発してヒト血清蛋白質の分離に成功し,抗体の研究等の功績によって1937年ノーベル化学賞を受賞した.本邦では平井と島尾がチゼリウス型電気泳動装置の組み立てに成功して電気泳動法を用いた血清蛋白質の分析と医学への応用が始まった.1950年電気泳動研究会が創設され,翌年に機関誌『生物物理化学』が創刊された.1959年中村らが世界に先駆けて交叉泳動法を開発し,電気泳動法による酵素‐基質,抗原‐抗体,核酸‐蛋白質などの複合体の分析を報告した.この方法は後に親和電気泳動法として生物特異結合反応の解析に広く応用され,生命科学研究の有力な技術となっている.
  
241-250
A Mathematical theory of steady state of moving boundary electrophoresis was described in detail and the theory was applied to the Tiselius' moving boundary of serum albumin in Barbital and phosphate buffers. The theory showed that the steady boundary could be formed in the ascending but not in the descending boundary. The changes of concentrations of the protein and buffer components, conductance and pH along the direction of electrophoresis were calculated for the ascending boundary and the results were in agreement with experiments.
  
電子冷却装置による電気泳動槽の冷却
  • 勝見 世英子, 松本 重一郎
  • 生物物理化学
  • Vol. 12 (1966) No. 1
  • 公開日: 2009年03月31日
63-64
1) Tiselius式電気泳動装置の恒温水槽に電子冷却装置をくみ合わせ,水槽温度の制御を試みたところ,水槽温度を0℃附近に保持し,かつ鮮明な泳動図を得ることができた。
2) このために実験上注意すべき点若干を明らかにした。
  
1.Tiselius装置組立ての懐旧
159-161
電氣泳動法に關する基礎的研究に就て
4-14,46
電気泳動学会50年
59-65
The Society of Electrophoresis was founded in 1950 by a group of the researchers in Japan who were engaged with the Tiselius Electrophoresis, and the name of the Society was changed to The Japanese Electrophoresis Society in 1993. The main activities of the Society include biannual scientific meetings (the General Meeting and the Spring Meeting), publications of the Physico-Chemical Biology (renamed to the Japanese Journal of Electrophoresis since 1995) and the monographs on Electrophoretic Methods, proposal of the standard electrophoretic methods (Tiselius, filter paper and cellulose acetate electrophoresis, and the standard films for densitometry), annual training courses of electrophoresis for beginners since 1963, and presentation of two types of academic awards (the Kodama Prize and the Hirai International Prize).
  
血清アルブミンの易動度及荷電の温度變化
120-122
Electrophoretic mobility of crystalline horse serum albumin was determined with Tiselius apparatus at various temperatures between 0°C and 30°C Electric charge per molecule of the protein was calculated by the application of moving boundaries. The results shows that electrophoretic mobility of the protein is approximately inversely inversely proportional to the viscosity of the solvent, while small deviations from this behavier can be interpreted as the displacement of electrolytic discossiation of the protein caused by the difference in temperature. This can also be proved by electric charge of the protein as calculated from electrophoretic mobility using the theory of moving boundaries.
  
正常日本人成人血漿の電気泳動分画
299-306
Plasma protein fractions of forty six cases of healthy adult Japanese, male and female, between 18-59 years old, were analyzed by Tiselius as well as cellulose acetate membrane electrophoresis.
The results were as follows:
In comparison with the nomal values determined before 1955, present data indicated definitely an increase of albumin and a decrease of γ-globulin.
The accuracy of the both methods was also checked by repeating the determination.
  
新方式Zone-Electrophoresisに関する研究
241-248
Electrophoretic diagram of human plasma was obtained with the apparatus using electrophoresis under density gradiet of dextran. Plasma protein was separated into sixteen fractions by either veronal buffer at pH 8.6 and ionic srength 0.1 or sodium bicanate buffer at pH 8.37 and ionic strength 0.05. While, only ten clearly separated fractions were detectable with phosphate buffer at pH 7.8 and ionic stength 0.144. Quantity and mobility of each fraction were determined and the result was disscussed in relation to that obtained by Tiselius apparatus.
  
新方式Zone-Electrophoresisに関する研究
58-62
1. For the purpose of improving the ability of electrophoretic separation of the Tiselius apparatus, the author developed a method by which to form a dependable zone between solutions in cells. At the same time he obtained a particular zone electrophoretic diagram by means of an optical apparatus of his own making.
2. From the viewpoint of relative mobility, at least new 10 peaks were observed, in addition to 6 (A, α1, α2, β, φ and γ) which have been found.
3. Though these new peaks may be regarded as sub-fractions of the said fractions, the author now desists from forming his conclusion.
  
手作りの電気泳動法50年
67-72
During fifty years' history of the Japanese Electrophoresis Society, many types of handmade electrophoresis experiments by many members of the Society contributed to the progress of electrophoretic methods. Starting from the handmade Tiselius' electrophoresis apparatus by Hirai and Shimao, recollections of the members active in the handmade electrophoreses in the first fifteen years of the Society are described. Then, two examples of handmade electrophoresis by the author, namely, a buffer gradient gel electrophoresis and a comparative study of computer simulations of glycine and tricine discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis made in the last twenty years are shown.
  
寒天内における蛋白質等の電気泳動法
  • 平井 秀松, 鈴木 佳枝
  • 生物物理化学
  • Vol. 3 (1956) No. 2
  • 公開日: 2009年03月31日
138-141
Using U-tube electrophoresis it is not easy either to separate certain fraction in sufficient amount or to analyze low molecular substances. To avoid these disadvantages the auther attempted the electrophoresis in an agar gelly column.
Some quantitive analytical results of serum proteins showed good coincidence with those by Tiselius electrophoresis and extaction of certain desired fraction of the protein from agar column was made successfully.
Analysis of human saliva indicated that the amylase of saliva is a single component electrophoretically.
Mixture of certain amino acids in definite amount was analyzed. Results indicated satisfactory separation.
This method may particularlly be available for electrophoretic purification of subjected substance in relatively larger amount.
  
268-277
我々は濾紙電気泳動法における各分屑の動きを理論的並に実験的に考察したが,これを要約すれば,
1) Kunkel & TiseliusおよびMacheboeuf一派の考え方を更に一般化することの可能性を理論的に示した.
2) Macheboeufの蒸発効果は專らJoule熱によるものであり,従ってどのような型式の濾紙電気泳動法でも彼等の整一蒸発に近い状態にあることを明らかにした.
3) 濾紙法において血清蛋白分屑の相対移動度の決定或いはその同定に就て述べ,α2-Glob.の相対移動度がfree Tiselius法より常に15%低いことを注意した.
  
実験的腹水の蛋白像に関する研究
259-267
The author has studied the protein pattern of some experimental ascites induced by various methods by classical Tiselius procedure and paper electrophoresis and has obtained the following results.
1) Animals having an accumulation of ascitic fluid did not necessarily reveal hypoalbuminemia, but in general they showed higher percentage of albumin in ascitic fluid than in the peripheral blood.
2) Generally speaking, the protein patterns of concomittant ascitic fluids resembled that of serum regardless to the inducing procedures. Careful observation, however, indicates that in most cases, albumin percentage is higher in the ascites than in the circulating blood, but in some cases about equal or higher in the serum.
  
肺結核患者の血漿蛋白像に及ぼすチビオンの影響
  • 向井 壽徳, 山田 榮士郎, 工藤 卓哉, 吉田 司, 白井 正敏
  • 生物物理化学
  • Vol. 1 (1951) No. 1
  • 公開日: 2009年03月31日
30-34,46
We studied the plasma protein fraction of healthy men and of patients of pulmonary tuberculosis by means of Tiselius' apparatus. We admihistered tibione for 1-4 months to the patients of pulmonary tuberculosis, and observed the change of plasma protein fraction, with various clinical investigations.
The results are as follows;
The total protein, albumin and albumin-globulin quotient decreased, but γ-globulin and fibrinogen increased more in the plasma protein fraction of the cases of pulmonary tuberculosis than in those of healthy men.
When tibione gave effective results, pathological change of plasma protein fractions had a tendency to return to healthy conditions.
  
透析操作を省いた迅速電気泳動法について
  • 吉田 鉄郎, 高橋 重郎
  • 生物物理化学
  • Vol. 5 (1958) No. 2
  • 公開日: 2009年03月31日
61-68
A method for dispensing with the dialysis of serum and plasma samples prior to electrophoresis according to Tiselius' method in free solution has been described.
One volume of the sample was mixed with threee volumes of the phosphate buffer solution, and the mixture was analyzed by using a certain buffer solution (pH 7.9 and μ 0.15) as the supernatant in the electrophoretic run.
The electrolyte compositions of these buffer solutions were shown.
In cases where no pathological changes in the electrolyte composition of the blood cun be observed, a close agreement between electrophoretic analyses carried out accoring to this method and according to the conventional one with dialysis has been demonstrated on eight samples of the sera of healthy men and on eight samples of the sera and on four samples of the plasmas (obtained by means of heparinazed blood) of patients of various diseases.
  
細胞顆粒の界面に関する研究
278-283
The interfacial potential of cytoplasmic granules, i. e., mitochondria, microsome and of ultra-microsome, was studied by means of classical moving boundary method and Tiselius' electrophoretic method, and the following results were obtained.
1. In Miller-Golder's buffer of Γ/2=0.1, the isoelectric-point was about pH 4.5.
2. In neutral or alkaline solution, these particles were negatively charged.
3. It seems to the author that owing mainly to this negative charge of the particles that cytoplasmic granules, such as microsome and ultramicrosome, can stably exist in cytoplasm, are basophilic, and do not agglutinate when the pH of mediumm is kept alkaline during cell fractionation.
4. Judging from the isoelectric points of granules, it may be supposed that there exists about equal amounts of lecithine and cephalin on the surface of granules, however, further experiments are necessary to determine this precisely.
  
健康新生兒血漿蛋白質の電気泳動法的研究
  • 橋元 祐二, 永好 千鶴子, 緒方 昌一
  • 生物物理化学
  • Vol. 2 (1954) No. 1
  • 公開日: 2009年03月31日
31-34
The results of an electropholetical study on 15 healthy newborns, with Tiselius' apparatus (Type HT-B, Hitachi made) are as follows:
Pooled plasma were dialyzed for 16-20 hours, before electrophoresis, against 1/20M phosphate buffer. Experimental conditions were were as follows: electric voltage 90-130V., electric current 7.5-11mA., time 45-70 minutes, temperature at 3-16°C., Diagonal-slit angle 25°.
When compared with children,
1) total protein showed remarkable decrease,
2) albumin little or no decrease,
3) globulin remarkable decrease,
4) α-globulin remarkable decrease,
5) β-globulin remarkable decrease,
6) γ-globulin little decrease or nothing,
7) fibrinogen decrease,
8) A/G, increase,
9) α-G/A, decrease,
10) γ-G/A and γ-G/T. P. showed no remarkable differences.
  
AlbuminおよびGlobulinpheresisに伴う血清蛋白質の変動について
  • 東 悳彦, 長谷川 福市
  • 生物物理化学
  • Vol. 5 (1958) No. 2
  • 公開日: 2009年03月31日
41-46
In order to obtain some additional findings in the research for the formation of plasma proteins, authors have carried out two types of experiments with dogs and rabbits which might be called albuminphersis and globulinpheresis. These have the similar character as plasmapheresis performed by Whipple and others. In alouminpheresis or globulinpheresis, a certain volume of blood was withdrawn from animals, and blood cells were separated from plasma by centrifugation, washed twice with saline, suspended in globulin or albumin solution respectively which had been previously prepared from pooled plasma of the same species, and the resulting mixture, having approximately the same volume and concentration of globulin or albumin as depleted blood, was injected back into amimals intravenously.
By these procedures, animals were brought under the condition in which albumin or globulin fraction was partially lost while other blood components were remained almost unchanged. Changes in serum proteins after these treatments were analyzed at intervals by the micro-Kjeldahl method and the electrophoresis using a Tiselius apparatus.
In this report, it has been shown that the decrease in albumin and the increase in α- and β-globulin have been observed with some differences in details between albumin- and globulinpheresis. Furthermore, on the basis of the results obtained, some discussions have been made about the formation of serum proteins in relation to the labile tissue protein.
  
濾紙電気泳動法の応用(第1報)
116-122,139
The apparatus devised consists of two buffer vessels, two Ag(AgCl)KCl-electrodes, two electrode vessels, a horizontal plate, a cover, and a supporter frame-work for filterpaper.
A few basic experiments were performed by means of this apparatus.
The buffer used was veronal-acetate buffer at pH8.6.
Results:
1) Veronal-buffer was found incomparably best adapted for filterpaper-electrophoretic analysis of blood serum.
2) 15 hours' electrophoresis under the current of 2mA or so was found to be most appropriate.
3) 0.02cc of serum was sufficient for the purpose.
4) The same buffer could be used 5 times in succession without any practical disadvantage.
5) The result obtained was approximately identical with that obtained by the use of the classical Tiselius apparatus.
6) The serum values obtained in 10 normal persons averaged Alb. 59.0%, α1-Glob. 3.7%, α2-Glob. 5.9%, β-Glob. 10.8% and γ-Glob. 20.6%.