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  • 全文: 細胞電気泳動

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シンポジウム
83-86
ヒトリンパ球の細胞電気泳動的研究
  • 橋本 信也, 鈴木 啓司, 前納 宏章, 田中 弘久, 佐々木 勝久, 斎藤 裕子, 阿部 正和
  • 生物物理化学
  • Vol. 18 (1974) No. 4
  • 公開日: 2009年03月31日
241-260
Cell electrophoresis is a useful method to measure electric charge on cell membrane and recognize precisely biological changes on the surface of cells. In the present paper, cell electrophoresis was used in an attempt to investigate various changes on the surface of human lymphocytes, which were separated from human peripheral blood by means of 3% gelatin sedimentation method, with the following results.
1. Cell electrophoretic mobility was 0.983±0.189μ/sec/V/cm in average of eighty one normal subjects. Human lymphocytes were shown to be heterogeneous electrophoretically.
2. Cell electrophoretic mobility of the human lymphocytes from patients with lymph tissue disorders was investigated. Electrophoresis of four cases of acute myelogenous leukemia (AML) did not show significant change in both electrophoretic mobility and cytopherogram. A case of AML in which 70% of cells in peripheral blood were leukemic, however, showed a cytopherographically homogeneous pattern. The lymphocytes from giant follicular lymphoma and reticulosarcoma did not show marked changes.
3. Cell electropheretic mobility of the lymphocytes treated with anti-human lymphocyte antibody (ALS) was investigated. Cytotoxic antibodies were obtained from the pregnant female sera as judged by the micro-droplet lymphocyte cytotoxicity test. Electrophoresis was carried out after the lymphocytes were incubated with ALS. Electrophoretic mobility of the lymphocyte treated with high titer ALS was lower than that treated with low titer ALS. It was shown that lowering of electrophoretic mobility was correlated with cytotoxic activity of ALS.
4. Cell electrophoretic mobility of the lymphocytes sensitized with the anti-homologous γ-globulin and various anti-immunoglobulin polypeptide chains was investigated.
Electrophoretic mobility of the lymphocytes treated with anti-human γ-globulin was lower. The phenomenon was significantly found in the lymphocytes treated with anti-μ, anti-κ, and anti-λ polypeptide chain sera, but not so markedly in those treated with anti-γ or anti-α chain sera. However, electrophoresis of the lymphocytes treated with anti-γ and anti-α sera showed that the delay phenomenon of mobility occurred on some of the cells. From the results of the experiments, it was suggested that large amounts of μ-chain, κ-chain and λ-chain and small amounts of γ-chain and α-chain are present on the surface of human lymphocytes.
The results were discussed with comprehensive survey of literatures on cell electrophoretic investigations of human lymphocytes.
  
細胞電気泳動法の臨床応用
323-328
細胞電気泳動法の展望
149-156
細胞電気泳動法の臨床的応用
209-226
シンポジウム
49-51
リンパ球の電気泳動的解析
  • 清水 本武, 岩口 孝雄
  • 生物物理化学
  • Vol. 36 (1992) No. 6
  • 公開日: 2009年03月31日
329-337
2.細胞電気泳動法
209-217
49-58
Diagnostic significance of tanned sheep erythrocyte electrophoretic mobility test (TEEM) was studied for gynecological malignancy.
Lymphocytes from gynecological malignancy and non-malignancy were obtained by differential centrifugation and incubated with encephalitogenic protein reported by Field and Caspary. Tanned sheep erythrocytes were added to the supernates and electrophoretic mobilities were measured in an analytical cell microelectrophoresis apparatus (Cytopherometer, Zeiss).
Decrease of the electrophoretic mobility was more significant in the supernate from malignancy than in that from non-malignancy. The substance which reduced electrophoretic mobility of erythrocytes also inhibited macrophage migration.
  
正誤表
448g
正誤表
448f
正誤表
448e
正誤表
448d
正誤表
448c
正誤表
448b
正誤表
448a
リンパ球subpopulationによる細胞電気泳動試験の検討
  • 橋本 信也, 揚塩 正樹, 堀田 正一, 能勢 俊一, 松本 尚美, 小林 敏子, 阿部 正和
  • 生物物理化学
  • Vol. 27 (1983) No. 3
  • 公開日: 2009年03月31日
119-123
The supernatants of human peripheral blood lymphocytes stimulated with antigen and non specific mitogen influence electrophoretic mobility of sheep erythrocytes as indicator cells of lymphocyte sensitization. This test system has been used for application to clinical immunology.
Lymphocytes from human peripheral blood were obtained by centrifugation in Ficoll-Conray method. Then separation of T and non T lymphocytes was performed by the ordinary E-rosetting technique. Separated T and non T lymphocyte fractions were incubated with PHA, Con A and DNA obtained from calf thymus. To cultured supernatants the tanned sheep erythrocytes were added, and electrophoretic mobility of the sheep erythrocytes was measured in an analytical cell microelectrophoretic apparatus.
In the supernatant from normal T lymphocytes stimulated with PHA and Con A, decrease of electrophoretic mobility of the sheep erythrocytes was more remarkable than in that from normal non T lymphocytes. In the supernatants of the lymphocyte subpopulation from patients with SLE stimulated with Con A, delay of electrophoretic mobility of the sheep erythrocyte was observed in non T lymphocytes. When the lymphocytes from the patients with SLE were stimulated with DNA, electrophoretic mobility of the sheep erythrocyte showed change in the supernatants from T lymphocyte culture.
  
2.アガロースを用いた新しい細胞電気泳動の方法について
163-166
ヒトリンパ球剌激培養上清による細胞電気泳動試験
  • 橋本 信也, 揚塩 正樹, 堀田 正一, 能勢 俊一, 松本 尚美, 小林 敏子, 阿部 正和
  • 生物物理化学
  • Vol. 27 (1983) No. 3
  • 公開日: 2009年03月31日
109-118
Change of surface charge of the tanned sheep erythrocytes added with supernatants of human lymphocytes stimulated with antigens, mitogens or immunopotentiators was investigated. In supernatants of the sensitized lymphocytes stimulated with PPD, percentage slowing of electrophoretic mobility of sheep erythrocytes were increased. In normal lymphocytes cultured with PHA and Con A, there were high percentage slowings in electrophoretic mobility test, and in immunopotentiators such as OK-432, PSK, LVS, methyl B12, poly-A:U, poly-I:C and NaIO4, percentage slowings were not significantly changed.
Percentage slowing was reduced in RA lymphocytes stimulated with PHA, but not changed in SLE lymphocytes. In normal lymphocytes incubated with Lentinan, percentage slowing value was similar to the result obtained by Con A, but it was low in lymphocytes from the patients with collagen diseases and malignancies.
In SLE lymphocytes added with DNA from calf thymus and RA lymphocytes added with human IgG, electrophoretic mobility of sheep erythrocytes delayed.
  
教育シンポジウム
216-222