Anti
B. thuringiensis H-sera were prepared with
B. thuringiensis, Kurstaki (H-3a3b3c) and
B. thuringiensis, Aizawai (H-7) which are used in the BT microbial pesiticide formulations, in addition to
B. thuringiensis, Thuringiensis (H-1),
B. thuringiensis, Sotto (H-4a4b) and
B. thuringiensis, Israelensis (H-14). The prepared anti H-sera had relatively high specificity, and the titres of each anti H-sera ranged from 6, 400 to 12, 800.
Eighty-four strains of
B. thuringiensis isolated from various foodstuffs and farmland soil were identified with the 5 prepared anti H-sera. Consequently, 49 strains were identified by the anti H-sera, and included 38 of serovar H-3a3b3c, 7 of serovar H-7, 3 of serovar H-4a4b and 1 of serovar H-14.
Particularly, strains presumed as serovar H-3a3b3c were frequently detected from imported corn and from native vegetables and soil. This suggested that BT formulations were applied to various crops as preharvest and/or postharvest pesticides, and that attention should be paid to the contamination of
B. thuringiensis from the aspect of food hygiene.
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