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  • 粟津 邦男, 鈴木-吉橋 幸子
    日本レーザー医学会誌
    2004年 25 巻 2 号 79-82
    発行日: 2004年
    公開日: 2006/11/02
    ジャーナル フリー
  • 佐藤 出, 吉橋 幸子, 鈴木 幸子, 間 久直, 粟津 邦男
    レーザー研究
    2007年 35 巻 6 号 382-387
    発行日: 2007/06/15
    公開日: 2014/03/26
    ジャーナル フリー
    Matrix-assisted laser desorption/ionization (MALDI) is an important method to analyze such biomolecules as proteins. In particular, MALDI using an infrared laser (IR-MALDI) is expected to measure various samples such as sugar chains, lipids, and cells because a molecular vibration can be directly excited by IR laser. We developed an IR-MALDI time-of-flight mass spectrometer (TOFMS) using a tunable IR laser (Difference Frequency Generation laser: DFG laser) that can adjust wavelength from 5.5 to 10 μm. The mass-to-charge spectra of insulin with a succinic acid matrix can be taken at a wavelength of 5.8 μm, which differs from the infrared absorption of succinic acid. With a urea or sinapic acid matrix, insulin spectra are detected by irradiating the DFG laser with the wavelength shifted from the absorption of these matrices.
  • 内藤 康秀, 粟津 邦男
    レーザー研究
    2003年 31 巻 1 号 16-20
    発行日: 2003年
    公開日: 2006/02/03
    ジャーナル フリー
    This commentary article provides short accounts of matrix-assisted laser desorption/ionization (MALDI) and time of flight mass spectrometry (TOFMS), particularly perspective views of MALDI using an infrared light source (IR-MALDI), and MALDI in conjunction with a tunable infrared free-electron laser (FEL-MALDI) as a state-of-the-art IR-MALDI methodology. The benefits and precautions to use FEL in MALDI are discussed. A novel approach to address the low-sensitivity issue of FEL-MALDI, simultaneous exposure to UV and FEL, is proposed. In the proposed method, each laser can be adjusted to minimize the excess energy under the conditions of rapid heating, which allows analyte molecules to be desorbed and ionized. The current status of the development and preliminary data are reported.
  • 鈴木-吉橋 幸子, 内藤 康秀, 石井 克典, 粟津 邦男
    レーザー研究
    2003年 31 巻 12 号 835-840
    発行日: 2003年
    公開日: 2006/02/03
    ジャーナル フリー
    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a powerful and robust tool for protein identification. We proposed a novel MALDI method (UV/FEL MALDI) based on simultaneous irradiation of a nitrogen laser (UV) and a free electron laser (FEL) to perform selective ionization, improving sensitivity and extending the upper limit of detectable molecular weight. Simultaneous exposure of UV and FEL showed some advantages over conventional MALDI cases. First, tuning FEL to an absorption wavelength of the matrix advanced the ionization efficiency. Second, multimers of keratin were observed up to approximately 900 kDa. These results suggest that UV/FEL-MALDI can be a more effective method for functional analysis of macromolecules and supramolecules of proteins than conventional used of MALDI.
  • 部谷 学, 内園 岳志, 佐野 秀, 石井 克典, 岩本 由美子, 熊崎 洋晃, 鈴木-吉橋 幸子, 粟津 邦男
    レーザー研究
    2003年 31 巻 12 号 805-810
    発行日: 2003年
    公開日: 2006/02/03
    ジャーナル フリー
    A mid-infrared Free Electron Laser (MIR-FEL) has potentials for medical application research. The MIR-FEL has great two advantages: (1) tunability and (2) double pulse structure. The Osaka MIR-FEL is tunable from 5 to 17 μm, and produces a macropulse, composed of a train of micropulses, each approximately 5 ps long and separated by 44.8 ns. (1) Since there exist main absorbers such as water, protein, and apatite in the tissue, wavelength tunability leads to a choice of the primary absorber. (2) Laser-tissue interactions are mainly determined by the power density of the laser used. Therefore the interactions can be precisely controlled by a combination of the energy density and pulse duration of the macropulse, and the repetition rate of the micropulse. Thus, the MIR-FEL can carefully optimize the laser conditions for a non-invasive treatment without accompanying side and adverse effects by individually controlling the FEL parameters (wavelength, power density, repetition rate).
  • 内藤 康秀, 粟津 邦男
    レーザー研究
    2003年 31 巻 12 号 799-804
    発行日: 2003年
    公開日: 2006/02/03
    ジャーナル フリー
    As an emerging field of molecular biology, proteomics gives a great impact on pharmaceutics, medicine and life science. The objectives of proteomics include protein identification and quantification, profiling of expression dynamics and localization, mapping of post-translational modifications, and probing of protein-ligand interactions. Because of tremendous complexity and diversity of protein world, proteomics requires high-throughput analytical technologies, which basically consist of gel electrophoresis, in-gel digestion, mass spectrometry and bio-informatics based on genome database. There are two aspects in which mid-infrared free-electron laser (MIR-FEL) can facilitate proteomics study: being used as a desorption laser in matrix-assisted laser desorption/ionization (MALDI) and as an activation laser in infrared multi-photon dissociation (IRMPD). This commentary article describes how MIR-FEL can be of benefit to proteomics, and indicates an ideal approach utilizing MIR-FEL to the goal of proteomics.
  • 内藤 康秀, 佐々木 理江, 部谷 学, 粟津 邦男
    レーザー研究
    2003年 31 巻 3 号 219-224
    発行日: 2003年
    公開日: 2006/02/03
    ジャーナル フリー
    A use of a free-electron laser (FEL) as the source of selective vibrational excitation was examined for infrared matrix-assisted laser desorption/ionization time of flight mass spectrometry (IR-MALDI-TOFMS). A polycrystalline mixture of fumaric acid/potassium chloride was exposed to FEL macropulses (15 μs). To make comparison, a pulse TEA-CO2 laser was also employed for the light source of IR-MALDI-TOFMS. In the present study, both the FEL and CO2 lasers were tuned at 10.2 μm. Preliminary results and novel findings are reported. The ability of ionization in the mid-infrared wavelength was evidenced by the mass spectrometric detection of fumaric acid ions. Contrastive absence and presence of low m/z peaks in the TOFMS spectra imply that a softer situation of desorption-ionization was achieved by exposure to the FEL than that to the CO2 laser. A novel ionization process via absorption of a single micropulse was suggested to be possible.
  • 医用電子と生体工学
    2001年 39 巻 Supplement 号 516-523
    発行日: 2001/05/09
    公開日: 2011/10/14
    ジャーナル フリー
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