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  • 平石 春義, 玉井 忠
    医療
    1974年 28 巻 8 号 675-680
    発行日: 1974/08/20
    公開日: 2011/10/19
    ジャーナル フリー
    Kaolin-agglutination test by Takahashi evaluated as a serodiagnostic test specific for tuberculosis was reexamined on the problems of preparation of the test solution, preservation of serum and conditions of measurement. Further, pattern of agglutination in the positive reaction of the test and γ-globulin content of serum related to antibody titer were studied in comparison of tuberculous disease with the nontuberculous one.
    The physiological saline solution used in preparation of THE saline solution, which should be made from chemically pure sodium chloride and non-ionic water, could be substituted with the physiological saline solution for injection prepared in the pharmaceutical company without making an error in the reaction. The serum was preserved in frozen state in which was antibody titer of the serum kept more stable than in hypothermic state at 4°C and kept constant for about ten days.
    For centrifugal sedimentation of the sample after the end of the reaction, 1, 800 to 2, 000 r. p. m. (for 5 minutes) in revolution of centrifuge were reconfirmed to be necessary and enough as a condition of sedimentation.
    It was noticed in the measurement of antibody titer that the sample should be so slightly shaken before the measurement as a sediment on the bottom of test tube is not broken to fine particles and that the same procedure, if the sediment were too much dispersed, should be taken after the second centrifugal sedimentation of the sample so as not to produce an error into titer measured.
    In representation of positivity of the reaction, not only antibody titer but pattern of the positive reaction i. e. grade of agglutination in each dilution of the sample should be adopted, because the titer of serum was comparably high in some patients with non-tuberculous diseases as in the tuberculous one though the patterns of the reaction were considerably different in each other, γ-globulin contents of both of serum of tuberculous and non-tuberculous patients were measured, showing a parallel relationship with the antibody titer in the former but not in the latter.
  • 沓掛 伸二, 近藤 哲理, 安部 明郎, 島野 毅八郎
    医療
    1977年 31 巻 3 号 273-276
    発行日: 1977/03/20
    公開日: 2011/10/19
    ジャーナル フリー
    赤血球
    凝集反応
    (HAR)を利用したαフエトプロテイン(AFP)の測定法を検討するために, 肝細胞癌や他の種々の疾患患者の血清や腹水のHARによるAFP値をRadioimmunoassay (RIA)によるそれと比較した. RIAによるAFP値100ng/ml以上の検体で, HARでAFPは検出可能で, HARとRIAによるAFP値は, 全く一致するとはいえないが, 強い正の相関があつた. 特異性に関してはHBs Ag陽性, RA因子陽性, CRP陽性, 高蛋白血症, 高γグロブリン血症, 高血糖などの条件では偽陽性は出現しなかつた. HARは他の簡易的AFP測定法に比べて, 感度, 特異性ともに高く, AFPのスクリーニングテストとしてはすぐれた検査法と考える.
  • 山田 昇
    レプラ
    1958年 27 巻 2 号 127-135
    発行日: 1958/03/20
    公開日: 2008/06/30
    ジャーナル フリー
  • 西村 武, 市川 陽三, 千葉 忠二郎
    医療
    1957年 11 巻 8 号 638-643
    発行日: 1957年
    公開日: 2011/10/19
    ジャーナル フリー
    The present paper deals with the characteristics of cold-agglutinin, found in a particular case of blood-group B. The case was noted at the time of cross-match test, when its serum showed pan-agglutination to all groups of tested red cells, including group B.
    At room temperature, its serum agglutinated all types of human red cells tested. At 20°C, the serum agglutinated its own red cells and the reaction disappeared at 37°C. The reaction however, re-appeared when these sets of test-tubes were cooled down to 20°C. Thus the reversibllity of the reaction was apparent.
    The colder the temperature, the higher the agglutinin titer. With group B and O cells, titers of 64 were obtained at 0°C and at 5°C, titers of 16, 8, 4 and 2 were obtained at 10°C, 15°C, 20°C and 25°C, respectively. At 37°C, no reaction occurred.
    Once the agglutinins were consumed in the cold agglutination, the supernatant showed normal reactibility in cross-match tests. The absorbed agglutinins were eluted again from red cells with saline solution at high temperature.
    The agglutinin titer was fairly stable. It did not decrease even after 30 days in an icebox. The agglutinin was resistant to the heating at 60°C for 30 days, but not at 65°C.
    Normal specimens of group B tested for the control showed titers of cold-agglutinin raging from 1 to 16, and their average was 5. None of the control reacted at 20°C. The authors discussed the possibility of finding out such particular specimens, at the time of cross-match tests.
  • 田村 奈保美, 上芝 幸雄, 富本 敏夫, 二村 久
    医療
    1977年 31 巻 6 号 547-550
    発行日: 1977/06/20
    公開日: 2011/10/19
    ジャーナル フリー
    結核の疑いで紹介されてくる患児の中には原発性異型肺炎(PAP)がかなり含まれている. またPAPの原因は, M. pneumoniaeが最も多いといわれる. 一方昭和51年秋より昭和52年冬は, マイコプラスマ肺炎の周期的な流行の年にあたるといわれている. そこで昭和47年より昭和51年の5年間の状況について, 臨床的観察を行い,次の結果を得た.
    1)入院患者中PAPの割合は, 昭和47年, 昭和51年には各22.8%, 26.5%であり, 他の年は5%前後であつた.
    2)昭和51年, 18名のPAPのうち, マイコプラスマ肺炎と診断されたのは9名で, 50%にとどまつた.
  • 須子田 キヨ, 平野 憲正, 湯川 智, 須藤 昭子
    レプラ
    1961年 30 巻 2 号 81-88
    発行日: 1961/06/20
    公開日: 2008/12/10
    ジャーナル フリー
    Sera obtained from 86 leprosy patients were tested for the presence of circulating antibody by means of the Ouchterlony method. The antigens employed were filtrate Preparations of Youman's culture media of the H37Rv strain, M. bovis (BCG, 263), two strains of atypical acid-fast microorganisms isolated from the sputum of tuberculous patients and saprophytic microbacterial strains (scotocromogenic acid-fast bacilli and milk strain). The results were as follows:
    Fifty-four Specimens of serum from 86 leprosy patients reacted with the above antigens on agardouble diffusion test. Thirteen specimens of these 54 cases gave a positive reaction only with the antigen of H37Rv, and five specimens reacted only with the antigen of M. bovis. Two of the remaining 36 specimens reacted only with the antigens present in atypical acid-fast bacilli. The last 34 specimens of sera reacted with the common antigens present in H37Rv, bovine strains (BCG, 236), atypical acid-fast bacilli, milk strain and Dharmendra antigen.
    Antibodies to the antigens of atypical mycobacteria were detected also in sera of tuberculous patients as indicated in the preceding paper. The positive rate of the Ouchterlony test in sera of tuberculous patients was less than that in leprosy patients. In so far as these tests show, there is significant differences between the positive cases in the Ouchterlony test obtained in lepromatous and tuberculoid leprosy. For example fifty-one of fifty-four cases of a positive Ouchterlony test were observed in lepromatous leprosy and only the remaining three cases in tuberculoid. Sex, age and process of diseases as will as associated tuberculosis do not effect the results of the Ouchterlony test. Sera taken from healthy nurses did not show any precipitation band with the exception of one case. In this case no evidence of tuberculous disease had been noted in the past.
  • 河野 敏郎, 高橋 義行
    日本植物病理学会報
    1997年 63 巻 5 号 403-405
    発行日: 1997年
    公開日: 2009/02/19
    ジャーナル フリー
    The flocculation test using high density latex (HDLF) which gives a reaction figure very similar to that of sensitized sheep red blood cells was established for simplified detection of rice stripe virus from infected rice plants and viruliferous insect vectors. Furthermore, six other plant viruses such as rice dwarf virus, odontoglossum ringspot virus, cymbidium mosaic virus, turnip mosaic virus, carnation mottle virus and cucumber mosaic virus were detected by HDLF of each infected host. These dilution end points for the positive reaction of sap were 1:8×103 to 1:16×103. Though weak non-specific reactions of HDLF were observed in sap from some healthy plants, they could be prevented by diluting sap more than 1:100.
  • 植村 許子, 吉野 勇次
    レプラ
    1966年 35 巻 1 号 8-14
    発行日: 1966/01/30
    公開日: 2008/12/10
    ジャーナル フリー
    Numerous reports have already been published on the ABO blood type in leprosy patients and it has been stated that there is no significant difference in distri-bution compared to the Japanese in general.
    The blood type in Japanese patients in the Oshima seishoen was examined. The percentage was determined according to the X2-method and the results compared to the findings obtained by the same method in 1932.
  • (第2報) LL2株およびハワイ株の寒天内沈降反応による成績の比較
    須子田 キヨ, 平野 憲正, 中野 寿夫, 弥吉 真澄
    レプラ
    1966年 35 巻 1 号 21-26
    発行日: 1966/01/30
    公開日: 2008/12/10
    ジャーナル フリー
    The results of the 1st and 2nd report are summarized as follows. A mycobacterium was isolated from mice inoculated with human leprous material into the testis and extra-testis as shown in the first report. This bacillus is transmissible from mouse to mouse and unable to be cultivated on artificial culture media. Besides, LL2 was difficult to differentiate morphologically from murine leprous Hawaii. Pattern of the skin test on guinea pigs sensitized with the attenuated tubercle bacillus showed that antigen of the LL2 in mouse and antigen of the murine leprosy Hawaii are iden-tical. These two patterns are different from the Mitsuda antigen. But the antigen pattern of LL2 and antigen pattern of murine loeprsy Hawaii indicated different reactions by means of gel diffusion test. Therefore, it is believed that LL2 and murine leprosy Hawaii are of a different strain.
  • 中島 省一
    日本微生物學病理學雜誌
    1935年 29 巻 2 号 204-210
    発行日: 1935/02/01
    公開日: 2009/09/16
    ジャーナル フリー
    ちふす菌免疫血清竝ニぱらちふす菌免疫血清ヲ供試シ, ちふす菌竝ニぱらちふす菌ヲ用ヰテ夫々
    凝集反應
    ヲ檢査シ, 免疫血清卜免疫元トヲ相互ニ組合セ, 主
    凝集反應卜副凝集反應
    トヲ檢シ, 副
    凝集反應即チ類屬凝集反應
    ニ於テモ, 補體トシテ家兎若シクハもるもつと新鮮血清ヲ添加スレバ, 等シク凝集效價上昇ヲ來スコトヲ見タリ.
  • 阿部 正英, 松尾 一夫, 高橋 俊一郎, 稲葉 俊雄, 立川 昇
    レプラ
    1957年 26 巻 6 号 297-304
    発行日: 1957/11/20
    公開日: 2008/06/30
    ジャーナル フリー
    Lepromin reaction with Dharmendra antigen, Leproagglutination (Ogata) with the cardiolipin-lecithin antigen and Middlebrook-Dubos hemagglutination reaction were tested in 571 cases of leprosy (lepromatous type-436, tuberculoid type-135) and the relation of the intensity of the three reactions to the disease types and the interrelationship between the reactions were studied statistically.
    Lepromin reaction was negative in 391 of the 436 lepromatous cases (89.7%) and positive in 109 of 135 tuberculoid cases (80.7%).
    An endtiter higher than 1: 32 by Leproagglutination was observed in 83.2% of the lepromatous cases and an endtiter less than 1:16 was seen in 86.7% of the tuberculoid cases.
    The endtiter of Middlebrook-Dubos hemagglutination in relation to the disease type was not so definite as with the previous two reactions but the mean endtiter was higher in the lepromatous cases.
    Lepromin reaction was in a negative correlation to both Leproagglutination and Middlebrook-Dubos hemagglutination, the latter two were in a positive correlation. Significance of the above correlation among the three reactions in view of the nature of the disease, however, has not been cleared yet.
    Lepromin reaction and Leproagglutination are considered to be important means for determining the type of leprosy and by utilizing these two reactions, it will not only be possible to diagnose the disease type with greater accuracy, but also will contribute much to the immunological study of leprosy.
  • V. 体液性免疫 寒冷凝集素と肺疾患
    猿田 栄助
    医療
    1974年 28 巻 11 号 1010-1011
    発行日: 1974/11/20
    公開日: 2011/10/19
    ジャーナル フリー
  • 滝本 義一
    日本輸血学会雑誌
    1973年 19 巻 4-6 号 135-141
    発行日: 1973年
    公開日: 2010/03/12
    ジャーナル フリー
    Using tannic acid or bis-diazotized benzidine, Australia antigen was coated to glutaraldehyde-fixed human erythrocytes. The observed titers were comparable to those obtained with sheep erythrocytes, but clear cut pictures of negative hemagglutination were rot obtained in human erythrocytes. The indistinctness of settling patterns was not improved by decreasing of Au antigen or coupling reagents concentration, and also not by the addition of Gum arabic or Tween 80 to the agglutination medium. The advantages were, however, recognized that for the detection of anti-Au antigen in human sera the sensitized human erythrocytes were able to avoid difficulties caused by the presence in the sera being examined of hemagglutinins againt non-homologous erythrocytes and the test was read more rapidly in human cells than in sheep cells because of rapid production of settling patterns.
  • 西村 武, 菊池 公児, 千葉 忠二郎
    医療
    1961年 15 巻 2 号 153-155
    発行日: 1961年
    公開日: 2011/10/19
    ジャーナル フリー
  • 辻 正周, 中溝 保三, 奥山 雄介, 飯村 達, 清水 長也, 柳下 徳雄, 中島 邦夫, 足立 利幸, 新美 裕成, 保井 英憲
    感染症学雑誌
    1977年 51 巻 3 号 136-142
    発行日: 1977/03/20
    公開日: 2011/09/07
    ジャーナル フリー
    ASO titre (hemolysis inhibition reaction), Blue ASO titre (passive agglutination reaction) and ASK titre (Kinase test) of the paired serum specimens which were taken from 40 patients with scarlet fever were measured.
    A rise in antibody titre by more than 2 tubes in the course of illness was observed in 47.5% by ASO, in 75.0% by Blue ASO and in 42.5% by ASK.
    In 16 cases by ASO and 18 cases by ASK in which no change in antibody titre was observed, Blue ASO titre showed significant rise by more than 2 tubes in the couse of illness in 68.7%(11 in 16 cases) and in 66.7%(12 in 18 cases), respectively. However hard we tried to decrease negative diagnosis rate by combining the above 3 tests, we could not down it below 20%.
  • 春日 壽夫
    日本鑑識科学技術学会誌
    1999年 4 巻 1 号 37-41
    発行日: 1999年
    公開日: 2010/05/30
    ジャーナル フリー
      For ABO blood typing of human salivary stains, a new mixed agglutination reaction method was devised, in which, OHP film was used as antigen-antibody reaction plate. By this method a series of examination procedures such as extraction of blood group substances, heating-fixation of the substance and incubation with monoclonal antibodies could be performed successively on the OHP film. For the secretor's salivary stain samples, specific agglutination reaction which presents an agglutination circle was observed. ABO blood typing from secretor's salivary stains was correctly performed by this method.
  • TPHAテスト補遺
    畑 清一郎
    皮膚
    1970年 12 巻 1 号 97-101
    発行日: 1970年
    公開日: 2010/08/25
    ジャーナル フリー
    225 cases were serologically studied for syphilis. TPHA titers had no relation to VDRL and CF titers (Table 2). TPHA showed low but positive titers in old syphilis who got infected 30-50 years ago and congenital syphilis, while VDRL and CF were negative in some of them.
  • 第2報 血球の自家呼吸とその血球の凝集価との関連性について
    中川 洋, 新宮 正久
    ウイルス
    1958年 8 巻 2 号 100-110
    発行日: 1958/04/25
    公開日: 2010/03/16
    ジャーナル フリー
    Many interesting problems still remain to be studied in the field of viral hemagglutnation. The present authores have taken particular interest in the findings that viruses have specific affinity to the componen's of the erythrocytes. The biochemical studies of the metabolic process of erythrocytes treated with virus are to be made in parallel with electrostatical studies, and so the mechanism of the viral hemagglutination might be expected to be easily understood.
    The authores have made biochemical and electrostatical studies on viral hemagglutination.
    The present paper deals with the results of the manometrical experiment which have been undertaken on oxidative respiration of erythrocytes treated with virus. And it has been observed that the existence of a certain mathematical relationship between the endogenous oxidative respiration of erythrocytes and its hemagglutination titer.
    In fact, if, changing various conditions of hemagglutination (kinds of reaction medium; addition of serum or glucose to medium) and using various erythrocytes (those treated with trypsin, tannic acid, virus, or RDE), measurement is to be conducted on the influence of the endogenous oxidative respiration of erythrocytes on the hemagglutination titer, one may notice that hemagglutination titer decreases with the increase of the endogenous oxidative respiration, on the other hand, hemagglutination titer increases with the decrease of the endogenous oxidative respiration of erythrocytes.
    All these findings would indicate that there exists a common component, between the substrate of endogenous oxidative respiration enzyme end the important part of receptor of erythrocytes.
  • 松沢 茂隆, 田中 博子
    日本輸血学会雑誌
    1971年 18 巻 3-4 号 63-72
    発行日: 1971年
    公開日: 2010/03/12
    ジャーナル フリー
    Medium solutions for hemagglutinating tests reported herein have advantages such as potent enhancing effect toward incomplete hemagglutinin, relatively low viscosity and stable colloid conditions.
    Details of preparation methods of the media had been previously described. Compositions of the media are as follows:
    PF medium—7ml of 30% PVP k30 (pH 7.5), 2.0ml of 25% ficoll, 0.68g imidazole, 0.82g natrium chloride, 0.1g sodium azide, 0.01ml Tween 80, 85ml deionized water and an appropriate quantity of hydrochloric acid to adjust the pH of the mixture to 7.5.
    PFP medium—85ml of PF medium, 15ml of 30% PVP k15 (pH 7.5) and 2 to 5g bovine serum albumin (optional).
    (1) Serological properties of blood group agglutinins diluted with PF or PFP medium.
    The incomplete-agglutinin enhancing effect of PFP medium was weaker than that of PF, but stronger than 20 to 30% bovine serum albumin solution. After mixing with PF or PFP medium, most blood group agglutinins, e. g. anti-D, anti-Lea and anti-P1, caused the enhanced hemagglutinating reaction even outside their respective optimal temperatures. Marginally positive Le(a+) and P1(+) red cells became susceptible to react with corresponding agglutinins to form large sized and firm agglutinates. Owing to these effects, blood group determination due to agglutinin reaction was performed without reading difficulties when the reagents were diluted with PF or PFP medium. The mixture of blood grouping reagent and the PF medium sometimes caused weak agglutination of red cells obtained from persons belonging to uncorresponding groups. This disturbance was supposedly due to either or both (1) enhancement of dormant incomplete hemagglutinin, and/or (2) rouleaux formation. The former type of the nonspecific agglutination was in most cases caused by a kind of incomplete cold hemagglutinin, which persistently remained in normal or immune sera even after overnight storage of whole blood at 5°C. This type of disturbance did not occur after further absorption with red cells. The latter phenomenon was completely inhibited by adoption of the testing techniques in which excessive evaporation from the surface of the reacting mixture was prevented, e. g. tube test and slide test performed in a moist chamber. PFP medium hardly caused nonspecific hemagglutination.
    (2) Examples of application of PF and PFP media on blood group serology.
    1) Detection of blood group agglutinin activity from electrophoreticaly or chromatographicaly obtained fractions of antisera: Not infrequently, hemagglutinating activities of highly diluted solutions of fractionized serum components could not be detected by means of saline and albumin test or by the tests with enzyme treated red cells. Addition of PF medium to the mixture of the fractionized solution and red cell suspension promoted the agglutinin activity to feasibly determine the distribution of the agglutinin. This technique may be applied to many experiments in place of the Coombs' test.
    2) Detection of incomplete hemagglutinins for serological tests of ABO-incompatible pregnancy: Incomplete anti-A, anti-B and anti-C (of ABO) agglutinins in sera of pregnants were strongly enhanced by the addition of 1/3part of PF medium into the reacting mixture of tested sera and red cell suspension. Titers obtained by this test were 4 to 8 times higher than those by enzyme treatment test; whereas the sensitivity of PFP medium test was intermediate of the enzyme treatment test and albumin test.
    3) PF medium test for substitution of the indirect Coombs' test: Selected were 6 blood grouping reagents for which the use of Coombs' test was directed by the manufacturers. One drop each of the reagent, 3% red cell suspension and PF medium were mixed, allowed to stand for 20min at 25°C and centrifuged for 1min at 1.5000 r. p. m. Four of the antisera properly caused positive reaction of red c
  • 太田 真, 西村 武
    医療
    1963年 17 巻 2 号 73-81
    発行日: 1963年
    公開日: 2011/10/19
    ジャーナル フリー
    Between epidemy of C. R. -positive P. A. P. and agglutinin value of healthy people in the same district a sort of relationship can be found.
    On the beginning of the epidemy, remarkable high value cases can be found, but not on prolonged epidemy.
    On the beginning of epidemy, along the alteration of it, positivity and negativity of C. R. of healthy people seems to be inverse each other. But, by prolonged epidemy, if positivity is relative low, negativity is also low. And, till at least 2∼3 years after epidemy, negativity seems to remain low.
    By these facts, we believe to be able to inspect an epidemiological condition of P. A. P. of a district through C. R. results of so called healthy but latent infected people in the same district.
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