1) Glycerinated Merzonin vaccine, a rabies vaccine prepared through an in activation process with glycerin and Merzonin, proved to be slightly more potent than the original Merzonin vaccine. Inactivation by glycerin alone reduced the potency of the vaccine remarkably. The Merzonin vaccine was better reinforced in its potency by 1% gelatin than by 5-10% sucrose. The former could nearly substitute glycerin to produce a high potency vaccine.
2) Glycerinated Merzonin vaccine exhibited a greater resistance than the original Merzonin vaccine, when exposed to a wide temperature range. Its potency was not lowered by -10°C, nor by +43°C. Consequently, the vaccine was not only superior to the original one in its antigenicity, but it could be preserved better.
3) Even the ordinary Merzonin vaccine could maintain its original fiter at least for a year, when stored at 4°C. A sample, which had a potency of 2, 401, 100 (+) immediately after the production, still kept a potency of 10, 000 after 3 years' storage at that temperature. The phenolized vaccine, which was prepared after the “minimum requirement” and had a potency of 125, 900 at the time of production, lost its potency to 50, 100 already after 4 months, to 1/5 of the original fiter after a year and to only 158 (-) after 3 years. Phenolized vaccine, even when glycerinated, still evidently inferior in its antigenicity to the glycerinated Merzonin vaccine.
4) The potency of vaccine, prepared by ultraviolet irradiation from the same brain material employed for the Merzonin and the glycerinated Merzonin vaccines, was always slightly lower to that of the latter group.
5) Provided that the original vaccine is sufficiently potent, a certain degree of dilution or centrifugation was regarded to be applicable in a practical use without impairing antigenicity.
6) Twenty-six human cases bitten by rabietic dogs were inoculated with 0.2cc of 10% Merzonin vaccine 7 times in 4 to 7 days. Neither rabies nor accidental side effects of vaccination developed in any case.
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