The mechanism of rejection of allogeneic organ grafts is not fully understood. The rejection of grafted skin is complicated as skin epidermal cells express skin specific antigens. Thus skin epidermal cells are suitable target cells for analyzing skin graft rejection in in vitro studies.
In this study, ⁵¹Cr labeled skin epidermal cells were prepared from rat tail skin by trypsin digestion. The susceptibility of skin epidermal cells to immune spleen cells in a lymphocyte mediated cytotoxicity assay and LDA in an antibody dependent cytotoxicity assay was examined in allogeneic skin grafts in rats. Skin epidermal cells were specifically killed by immune killer lymphocytes or LDA in combination with normal rat spleen cells. The optimal condition for assays were 10 to 12 hours of incubation and a 100:1 to 200:1 effector to target cell ratio. When the kinetics of LDA activity after allogeneic skin graft was examined, LDA appeared in the recipient serum just after the rejection and maintained activity for more than 40 days after the skin graft.
To analyze the mechanism of graft rejection, it is resonable to use the same target cells as the graft itself in both cellular and humoral immunoassays. In this sense, skin epidermal cells are useful for the study of skin rejection.

抄録全体を表示

We report a patient with a sore throat and trismus associated with tetanus. The 71-year-old man had a sore throat and so consulted the hospital. However, no abnormal physical or fiberscopic findings were noted except for a high CPK level (438 U/l) in the serum. CT and MRI did not demonstrate abnormal findings. He showed trismus and convulsion after 6 days, and was then diagnosed with tetanus. Respiratory care involving intubation and the administration of Penicillin G (24 million units/day for 11 days) and human anti-tetanus immunoglobulin were performed, resulting in cure without any sequelae. We should consider tetanus in patients with a sore throat and trismus because one quarter of tetanus patients show no injury history.

抄録全体を表示

各種チオウレア誘導体のオゾン劣化防止効果を評価するため, エタノール中におけるチオウレア誘導体のオゾンによる消費速度, およびチオウレア誘導体を含むシス-1,4-ポリイソプレンゴム溶液のオゾン化酸素による粘度低下を測定した.その結果, (1) チオウレア置換体の置換基数がモノ, ジ, トリと増加するにつれて一定量のオゾンを通した場合のチオウレアの消費速度は減少し, ゴム溶液粘度低下防止率も下がる。 (2) 1,3-ジアルキルチオウレアのアルキル基が大きくなるにつれ, チオウレアの消費速度および粘度低下防止率が下がる.また, 同じ炭素数でも枝分かれしたアルキル基の場合には, 相当するn-置換体より効果は低下する. (3) 芳香族チオウレアはアルキルチオウレアに比し, いずれも粘度低下防止率は低かったが, その置換基定数と粘度低下防止率との間にはハメット則が成り立つことが明らかとなり, 電子押出し効果の大きい置換基を有するものほど粘度低下を抑制する効果が強いことが認められた.

抄録全体を表示