Metacercaiae of
Metagoniwus yokogawai parasitic on the sweetfish (
ayn) living in Abu River, Yamaguchi Prefecture were collected through the artificial gastric juice digestion method and every six groups of about 1000 metacercariae were artificially cultivated by using the following culture media.
A. 5% liquid glucose, 8ml+complex streptomycin, 0.08g+a piece of an uninfected rats intestine, 1g. Kept in a culture box (1822°C).
B. 5% liquid glucose, 8ml+complex streptomycin, 0.08g. Kept in a culure box (1822°C).
C. City water, 8ml+complex streptomycin, 0.08g. Kept in a culture box (1822°C).
D. Physiological solution of sodium chloride+complex streptomycin, 0.08g. Kept in a culture box (1822°C)
E. City water, 8ml+complex strptmycin, 0.08g. Kept in a room tempetature (520°C).
F. 5% liquid glucose, 8ml+complex streptomycin, 0.08g. Kept in a refrigerator (5°C).
In culture medium A larvae which have dropped off their cysts are seen to move actizely on the first two days. On the 7th day, larvae without cysts are still found. In cnlture medium E larvae without cysts are first found on the 18th day. As dead metacercariae increase in number, their existence is made sure of through the infection test on animals from the 9th day on. Culture fluid A is given to four rats 16 days after cultivation and then all of four rats are infected. Culture fluid C is given to three rats 32 days after cultivation and the result is negative. Culture fluid D is given to two rats 34 days after cultivation and the result is positive in both the hosts. Culture fluid F is given to two rats 14 days after cultivatin and the result is positive in both the hosts.
These results have proved that metacercaria of
Metagonimus yokogawai has a parasitic ability to a final host as late as 34 days after it leaves the sweefish.
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