As yet no reports have been made concerning the secretion of peptide within the pancreas during the utilization of artificial endocrine pancreas. This study was designed to investigate the changes in plasma thyrotropinreleasing hormone (TRH), pancreatic polypeptide (PP) and somatostatin (SOM) levels by the glucose
clamp
technique with artificial endocrine pancreas in 50 patients with non-insulin-dependent diabetes mellitus (NIDDM) (31 males and 19 females).
After an overnight fast, 90 min. euglycemic
clamp
was performed and followed by 90 min. hyperglycemic
clamp
. Blood samples were collected before and 30, 60, 90 min. after each glucose
clamp
to measure glucose, IRI, TRH, PP and SOM.
TRH showed no remarkable change throughout each of the glucose
clamp
. Plasma PP levels decreased significantly at 30 and 60 min. in the euglycemic hyperinsulinemic
clamp
only. Likewise, plasma SOM levels decreased significantly at 30, 60 and 90 min. in the euglycemic hyperinsulinemic
clamp
. And plasma SOM levels increased significantly at 30 and 60 min. in the hyperglycemic
clamp
.
Although it was not clear whether the mechanism of this phenomenon was due directly to the insulin effect or due to other factors, present results of the glucose
clamp
were quite different from those in previous acute effects of insulin loading.
The present study indicated that plasma TRH showed no significant change but that plasma PP and SOM showed significant changes from each glucose
clamp
using artificial endocrine pancreas in patients with NIDDM. It is suggested that these changes may be related to glucose level, insulin level and insulin sensitivity.
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