A screening method for deoxynivalenol (DON) residue in beer by use of a commercially available
ELISA
-kit was applied for
its accuracy management for method validation. DON in beer sample was subjected to acetonitrile extraction and cleanup with a
MycoSep #227 multifunctional column. Recovery experiments indicated that the trueness of the low concentration sample (10 ng/
mL) and that of the high concentration sample (100 ng/mL) were higher than 90%, respectively. The relative standard deviation
(RSD) of repeatability and that of intermediate precision were less than 25%, respectively. In addition, the interlaboratory precision
of seven laboratories was determined as an external quality control test with and without sample cleanup. The interlaboratory
precision of the high concentration sample (100 ng/mL) showed an acceptable Z-score (less than 2.0 and greater than -2.0;
|z| < 2) for all the seven laboratories, regardless of whether or not cleanup was performed. In addition, the gap between the
added concentration and the average value (most probable value) was less than 20%. On the other hand, in the case of the low
concentration sample (10 ng/mL) without cleanup, the Z-scores were “|z| < 2” for all the seven laboratories, but the residual variance
was large and the deviation from the most probable value was increased. However, by performing cleanup pretreatment, DON
concentrations down to 10 ng/mL could be measured by
ELISA
. Then, sixteen commercially available beer samples were subjected
to DON determination using the present
ELISA
and a confirmation test using GC/MS. DON was detected in 9 beer samples (56.3%);
the mean concentration was 14.3 ng/mL and the highest concentration was 54.8 ng/mL. The correlation coefficient (
r) was high at
0.887. The results suggest that the
ELISA
-kit with the cleanup method is useful for a screening of low level DON in beer.
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