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  • Mota
    ,
    Mota
    化学と教育
    1990年 38 巻 1 号 51-
    発行日: 1990/02/20
    公開日: 2017/07/13
    解説誌・一般情報誌 フリー
  • Shuichi Nakamura, Tohru Minamino, Nobunori Kami-ike, Seishi Kudo, Keiichi Namba
    BIOPHYSICS
    2014年 10 巻 35-41
    発行日: 2014年
    公開日: 2014/06/14
    ジャーナル フリー
    The bacterial flagellar motor generates torque by converting the energy of proton translocation through the transmembrane proton channel of the stator complex formed by
    MotA
    and MotB. The
    MotA
    /B complex is thought to be anchored to the peptidoglycan (PG) layer through the PG-binding domain of MotB to act as the stator. The stator units dynamically associate with and dissociate from the motor during flagellar motor rotation, and an electrostatic interaction between
    MotA
    and a rotor protein FliG is required for efficient stator assembly. However, the association and dissociation mechanism of the stator units still remains unclear. In this study, we analyzed the speed fluctuation of the flagellar motor of Salmonella enterica wild-type cells carrying a plasmid encoding a nonfunctional stator complex,
    MotA
    /B(D33N), which lost the proton conductivity. The wild-type motor rotated stably but the motor speed fluctuated considerably when the expression level of
    MotA
    /B(D33N) was relatively high compared to
    MotA
    /B. Rapid accelerations and decelerations were frequently observed. A quantitative analysis of the speed fluctuation and a model simulation suggested that the
    MotA
    /B(D33N) stator retains the ability to associate with the motor at a low affinity but dissociates more rapidly than the
    MotA
    /B stator. We propose that the stator dissociation process depends on proton translocation through the proton channel.
  • 香山 晃
    核融合研究
    1992年 67 巻 6 号 485-489
    発行日: 1992/06/25
    公開日: 2010/10/22
    ジャーナル フリー
    The most important and difficult part of materials research for fusion reactor is realized to be irradiation studies of fusion reactor materials.Irradiation studies of fusion reactor materials utilizing FFTF/
    MOTA
    , as one of Japan/U.S.A.Fusion Collaboration Programs, have important role to establish fundamental understanding of heavy irradiation effects on materials behavior and properties and to develop methods and technologies for advanced irradiation studies under fusion reactor environment.This paper briefly reviews the history, the state of the art, and the future of the FFTF/
    MOTA
    program.
  • 松浦 弘幸, 根本 哲也, 野田 信雄, 小井手 一晴, 伊藤 安海, 中野 正博
    バイオメディカル・ファジィ・システム学会誌
    2007年 9 巻 1 号 35-41
    発行日: 2007/07/31
    公開日: 2017/09/04
    ジャーナル オープンアクセス
    我々は,確率共鳴の原理で動くルーズカップリングモータの原理を提案した.細胞膜の間に付属したプロトンポンプの働きで,水素イオンの静電的膜電位を形成される.形成されたポテンシャルが,水素イオン流を加速し細胞内部に流入させる.水素イオンの電位勾配による細胞内へのプロトンの流入過程でで,多くのプロトンは,膜(グリカン膜と細胞膜)内に埋め込まれた
    motA
    に衝突する.
    motA
    膜複合体は,確率共鳴現象を生じる.この共鳴は,量子論的にはコヒーレント状態である.外力(水素イオンの
    motA
    への衝突)により生じる量子コヒーレント状態は,
    motA
    のポテンシャルエネルギー曲線を下方に押し下げる.これにより以前の基底状態は,もはや最低エネルギー状態ではなく,シフト後の新しい基底状態よりエネルギー的に高い状態となり,過剰となるエネルギーを放出する.これにより基底状態の下方への変位が維持され,これにより,鞭毛モータが回り続けるのである.
  • Teruyuki Yorioka
    Journal of the Mathematical Society of Japan
    2017年 69 巻 3 号 913-943
    発行日: 2017年
    公開日: 2017/08/04
    ジャーナル フリー

    Recently, David Asperó and Miguel Angel

    Mota
    discovered a new method of iterated forcing using models as side conditions. The side condition method with models was introduced by Stevo Todorčević in the 1980s. The Asperó–
    Mota
    iteration enables us to force some Π2-statements over H(ℵ2) with the continuum greater than ℵ2. In this article, by using the Asperó–
    Mota
    iteration, we prove that it is consistent that ℧ fails, there are no weak club guessing ladder systems, 𝔭 = add($\mathcal{N}$) = 20 > ℵ2 and MA1 fails.

  • Yuki Sudo, Hiroyuki Terashima, Rei Abe-Yoshizumi, Seiji Kojima, Michio Homma
    BIOPHYSICS
    2009年 5 巻 45-52
    発行日: 2009年
    公開日: 2009/06/12
    ジャーナル フリー
    Flagellar motor proteins,
    MotA
    /B and PomA/B, are essential for the motility of Escherichia coli and Vibrio alginolyticus, respectively. Those complexes work as a H+ and a Na+ channel, respectively and play important roles in torque generation as the stators of the flagellar motors. Although Asp32 of MotB and Asp24 of PomB are believed to function as ion binding site(s), the ion flux pathway from the periplasm to the cytoplasm is still unclear. Conserved residues, Ala39 of MotB and Cys31 of PomB, are located on the same sides as Asp32 of MotB and Asp24 of PomB, respectively, in a helical wheel diagram. In this study, a series of mutations were introduced into the Ala39 residue of MotB and the Cys31 residue of PomB. The motility of mutant cells were markedly decreased as the volume of the side chain increased. The loss of function due to the MotB(A39V) and PomB(L28A/C31A) mutations was suppressed by mutations of
    MotA
    (M206S) and PomA(L183F), respectively, and the increase in the volume caused by the MotB(A39V) mutation was close to the decrease in the volume caused by the
    MotA
    (M206S) mutation. These results demonstrate that Ala39 of MotB and Cys31 of PomB form part of the ion flux pathway and pore with Met206 of
    MotA
    and Leu183 of PomA in the
    MotA
    /B and PomA/B stator units, respectively.
  • 松井 秀樹
    核融合研究
    1992年 67 巻 6 号 508-511
    発行日: 1992/06/25
    公開日: 2010/10/22
    ジャーナル フリー
    Vanadium alloys are expected to be potential reduced activation materials for fusion power reactors. R&D works of vanadium alloy have been emphasized in FFTF/
    MOTA
    program, where a new simulation irradiation technique, Dynamic Helium Charging Experiment (DHCE), has been developed.This paper provides recent results from FFTF/
    MOTA
    program with the emphasis of helium effects on swelling and on helium embrittlement.
  • A. Kohyama, Y. Kohno, K. Asakura, M. Yoshino
    Materials Transactions, JIM
    1993年 34 巻 11 号 1061-1068
    発行日: 1993年
    公開日: 2007/05/23
    ジャーナル フリー
    Irradiation creep behavior of low activation steels, developed as structural materials for fusion reactors, was investigated. The objective of this study is to provide a fundamental understanding of the irradiation creep mechanism based on microstructural evolution under fast neutron irradiation. Pressurized tube creep specimens fabricated from tube segments were irradiated in the Fast Flux Test Facility (FFTF), Materials Open Test Assembly (
    MOTA
    ) during FFTF Cycles 11 and 12. This paper provides the first creep results obtained after FFTF cycle-11 irradiation. (2.25–3)Cr–(1–2)W bainitic steels and 12Cr–2W ferritic/martensitic steels showed equivalent or superior creep resistance to a modified 316 stainless steel, known as Japanese Prime Candidate Alloy (JPCA), under fast neutron irradiation up to 600°C. For the case of ferritic steels, with increasing Cr content, creep strain increased up to 7 Cr and further increments of Cr content to 8, 9 and 12% reduced creep strain. Swelling enhanced creep near peak swelling temperature of 410°C was observed. Preliminary TEM observation suggests that irradiation induced precipitation and void nucleation were enhanced by applied stress near peak swelling temperature.
  • 四竈 樹男, 佐東 信司
    核融合研究
    1992年 67 巻 6 号 519-523
    発行日: 1992/06/25
    公開日: 2010/10/22
    ジャーナル フリー
    Materials handling techniques fbr highly radio-activated materials are key issues to accomplish post irradiation examinations (P. I. E. s) of heavily irradiated materials.To perform P. I. E. s of FFTF/
    MOTA
    irradiated specimens in hot-cells has been a technical challenge and many efforts to develop those techniques have been devoted. This paper describes status of the art of the techniques in Oarai Branch of IMR and hose developed in the frame work of the FFTF/
    MOTA
    program.
  • 高橋 平七郎
    核融合研究
    1992年 67 巻 6 号 501-504
    発行日: 1992/06/25
    公開日: 2010/10/22
    ジャーナル フリー
    One of strategies for developing fusion reactor materials is to start from clear understandings of microstructural evolutions and of their mechanisms.The pure and model alloys group in FFTF/
    MOTA
    program cares for elementary and basic processes of radiation damage mainly from damage microstructural development.This paper provides recent results from
    MOTA
    program on austenitic steels.The importance of radiation induced segregation and radiation enhanced precipitation in microstructural evolution and phase stability is emphasized.
  • 松浦 弘幸, 藤正 巖, 根本 哲也, 中野 正博
    年次大会講演論文集
    2002年 2002.4 巻
    発行日: 2002/09/20
    公開日: 2017/08/01
    会議録・要旨集 認証あり
    We constructed a new Flagellar model based on Quantum Physics, and this model for the transduction of energy in bacterial flagellar motor was presented. We proposed that Lagrageian were made of the four main parts, i.e.
    MotA
    -FliG complex, water molecules, dissipative terms and rotor of Flagellar. In this model, the flux of ions across the channel interacts with the charges, which existed in the wall of ionic channel. In this results, stochastic resonance generated between the Mot-A Flig-G complex and the ions. The
    MotA
    -FliG complex produced travelling waves in one of the subcomponents of the motor, the C ring. This wave stabilized the cyclical movement of the channel which generated the rotating force.
  • Hitomi Komatsu, Fumio Hayashi, Masahiro Sasa, Koji Shikata, Shigeru Yamaguchi, Keiichi Namba, Kenji Oosawa
    Biophysics and Physicobiology
    2016年 13 巻 13-25
    発行日: 2016年
    公開日: 2016/01/28
    ジャーナル フリー
    電子付録
    FliF is the protein comprising the MS-ring of the bacterial flagellar basal body, which is the base for the assembly of flagellar axial structures. From a fliF mutant that easily releases the rod-hook-filament in viscous environments, more than 400 revertants that recovered their swarming ability in viscous conditions, were isolated. The second-site mutations were determined for approximately 70% of them. There were three regions where the mutations were localized: two in Region I, 112 in Region II, and 71 in Region III including the true reversion. In Region I, second-site mutations were found in FlgC and FlgF of the proximal rod, suggesting that they affect the interaction between the MS-ring and the rod. In Region II, there were 69 and 42 mutations in
    MotA
    and MotB, respectively, suggesting that the second-site mutations in
    MotA
    and MotB may decrease the rotational speed of the flagellar motor to reduce the probability of releasing the rod under this condition. One exception is a mutation in FlhC that caused a down regulation of the flagellar proteins production but it may directly affect transcription or translation of
    motA
    and motB. In Region III, there were 44, 24, and 3 mutations in FliG, FliM, and FliF, respectively. There were no second-site mutations identified in FliN although it is involved in torque generation as a component of the C-ring. Many of the mutations were involved in the motor rotation, and it is suggested that such reduced speeds result in stabilizing the filament attachment to the motor.
  • Mota
    , 田坂 興亜
    化学と教育
    1989年 37 巻 5 号 547-
    発行日: 1989/10/20
    公開日: 2017/07/13
    解説誌・一般情報誌 フリー
  • Hitoshi NISHIMURA, Naoya MAKIBUCHI, Kazuyuki TASAKA, Yasutomo KAWANISHI, Hiroshi MURASE
    IEICE Transactions on Information and Systems
    2020年 E103.D 巻 6 号 1265-1275
    発行日: 2020/06/01
    公開日: 2020/06/01
    ジャーナル フリー

    Multiple human tracking is widely used in various fields such as marketing and surveillance. The typical approach associates human detection results between consecutive frames using the features and bounding boxes (position+size) of detected humans. Some methods use an omnidirectional camera to cover a wider area, but ID switch often occurs in association with detections due to following two factors: i) The feature is adversely affected because the bounding box includes many background regions when a human is captured from an oblique angle. ii) The position and size change dramatically between consecutive frames because the distance metric is non-uniform in an omnidirectional image. In this paper, we propose a novel method that accurately tracks humans with an association metric for omnidirectional images. The proposed method has two key points: i) For feature extraction, we introduce local rectification, which reduces the effect of background regions in the bounding box. ii) For distance calculation, we describe the positions in a world coordinate system where the distance metric is uniform. In the experiments, we confirmed that the Multiple Object Tracking Accuracy (

    MOTA
    ) improved 3.3 in the LargeRoom dataset and improved 2.3 in the SmallRoom dataset.

  • Yuka Takahashi, Kotomi Koyama, Masahiro Ito
    The Journal of General and Applied Microbiology
    2014年 60 巻 4 号 131-139
    発行日: 2014年
    公開日: 2014/10/01
    ジャーナル フリー
    The bacterial flagellar motor is mainly energized by either a proton (H+) or sodium ion (Na+) motive force and the motor torque is generated by interaction at the rotor-stator interface.
    MotA
    /MotB-type stators use H+ as the coupling ion, whereas MotP/MotS- and PomA/PomB-type stators use Na+. Bacillus subtilis employs both H+-coupled
    MotA
    /MotB and Na+-coupled MotP/MotS stators, which contribute to the torque required for flagellar rotation. In Escherichia coli, there is a universally conserved Asp-32 residue of MotB that is critical for motility and is a predicted H+-binding site. In B. subtilis, the conserved aspartic acid residue corresponds to Asp-24 of MotB (MotB-D24) and Asp-30 of MotS (MotS-D30). Here we report the isolation of two mutants, MotB-D24E and MotS-D30E, which showed a non-motile and poorly motile phenotype, respectively. Up-motile mutants were spontaneously isolated from each mutant. We identified a suppressor mutation at MotB-T181A and MotP-L172P, respectively. Mutants MotB-T181A and MotP-L172P showed about 50% motility and a poorly motile phenotype compared to each wild type strain. These suppressor sites were suggested to indirectly affect the structure of the ion influx pathway.
  • 耐鹽酸ニツケル合金の研究(其三)Ni-Mo(30%)合金の鹽酸による溶解度に及ぼす他元素添加の影響
    遠藤 彦造, 板垣 彰
    日本金屬學會誌
    1939年 3 巻 8 号 294-313
    発行日: 1939年
    公開日: 2008/11/13
    ジャーナル フリー
    First of all in this experiment, the effect of addition of various amounts of Mo to Ni on the dissolution rate of Ni by hydrochloric acid under following conditions, in 10 percent and 20 percent boiling solutions, and in 10, 20 and 30 percents solutions at room temperature, was systematically studied and compared the resistivities of this binary alloys of various compositions with those of Hastelloy A and Nemic, the latter was prepared by the present writers and its quality was also mentioned in the fifth report. Those alloys containing more than 35 percent Mo have high degree of resistivity against concentrated hydrochloric acid at room temperature and in the solutions of various concentrations under boiling condition, although they can be hardly cast and forged. But the alloy containing 30 percent Mo could be cast, forged and rolled with a special precaution and has also a good resistivity under all conditions mentioned above. Then the improvement of the resistivity of the alloy Ni-Mo (30%) was undertaken, i. e. the respective effect of Cu, W, Cu-Be, Fe-Ta, Fe-Ti, Fe-V and Fe-Zr, which were added to replace some part of Ni in the alloy, on the resistivity against the acidic solutions under conditions mentioned above and then pure metals, Ta, Mo, W and Ag were studied and compared with the above alloys. From each series of the alloys, six or seven kinds of composition were prepared making the total of the alloys studied to be 42 kinds. As every solution in all conditions has no oxidizing action, the effect of Cr was not studied. According to these experiments, the resistivities of MO40 (Ni-40% Mo) cast alloy against the boiling hydrochloric acid of 10 and 20 percents was the greatest and the next was MOW3, MOW5, either of these alloys were of cast alloy and superior in quality as that of Nemic alloy.
    As forgeablealloys, the qualities of MOV 0.3, MOV 0.5 and
    MOTa
    1 were similar to that of Nemic and MOV 0.5 has appreciably better resistivity then that of Nemic in 10 percent boiling solution. In the solution of 10 percent at room temperature, those of the alloys studied in these experiments were not so good as those of Nemicle C and Necomicle alloys, mentioned in the second report, prepared by the present writers, but those of forgeable alloys MOZ1, MOZ2 were a little better than that of Nemic alloy in the solutions of 10 and 30 percents. As cast alloys, those of MOV5, MOTi5 and MOW10 were also superior to that of Nemic, especially that of MOW10, which was twice better than Nemic in 30 percent solution. The famous alloy Hastelloy A was not so good as those of Nemic and the alloys mentioned above. After all, under room temperature condition, Cu, V, Ta and W have a beneficial effect and under boiling condition, Cu, Zr, V and Ti gave good effect on the resistivity of the allloy Ni-Mo (30%) against hydrochloric acid.
    Pure metals, Mo, W, and Ta were hardly attacked by 10 percent boiling solution and by 10, 20 and 30 percents solutons at room temperature but attacked by 20 percent solution under boiling condition. On the other hand, Ag was attacked by as much as that of Nemic by the boiling solutions of 10 and 20 percents, and the resistivity of Ag against the solutions at room temperature was the better, the stronger the concentration of the solution used.
  • 藤倉 輝道, 國友 万由美, 山口 潤, 鶴窪 一行, 稲葉 真, 大塚 博邦
    日本鼻科学会会誌
    1993年 31 巻 2 号 259-263
    発行日: 1993/03/01
    公開日: 2010/03/11
    ジャーナル フリー
    We have examined the influence of preservation before fixation and the procedure of making permanent section on the staining properties of mast cells in human nasal mucosa. Operative specimens of human nasal mucosa was fixed in MLA (
    Mota
    's basic lead acetate fixative). And sections were stained with toluidine blue solution.
    1. The influence of preservation of specimen in saline upon the stainability of mast cells.
    Before fixation a part of specimen was rinsed and preserved in saline for 1 hr. Another part of specimen was fixed directly in MLA without preserving in saline. There was no significant differnce of mast cell counts whether specimen was preserved in saline before fixation or not.
    2. The influence of mounting procedure on the staining property of mast cells.
    After fixation in
    Mota
    's BLA fixative, sections were stained with toluidine blue PH0.5. One drop of toluidine blue was put on the section and immediately mast cell count was done for 20min. After counting mast cells, the same section was rinsed in water and taken through graded alcohls to xylene and mounted in coverbond malinol. Then mast cell counting was done again. The number of mast cells in permanent section were fewer than that of mast cells counted immediately after staining. About 20% of mast cell stainability was reduced after mounting procedure. The reduction of stainability was seen in epithelial layer and in lamina propria at same grade.
  • 中村 修一, 南野 徹
    化学と生物
    2011年 49 巻 1 号 22-31
    発行日: 2011/01/01
    公開日: 2012/01/01
    ジャーナル フリー
    大腸菌やサルモネラ菌などの細菌は,「べん毛」と呼ばれる繊維状の運動器官をスクリューのように高速回転させて水中を泳ぐことにより,栄養が豊富で生育に適した温度やpHの環境に集まることができる.べん毛繊維を回転させているのは,繊維の根元に細胞膜に埋まって存在する「べん毛モーター」と呼ばれる直径約45 nmの回転分子モーターである.べん毛モーターは,細胞膜を隔てて形成される水素イオン(プロトン)の電気化学的ポテンシャル差を1秒間に約300回転の高速回転に変換し,その回転方向は環境中の化学物質などに反応して瞬時に切り替わる.ここでは,プロトン駆動型細菌べん毛モーターの構造と回転機構について,最新の研究内容を含めて紹介する.
  • Miguel
    Mota
    CYTOLOGIA
    1963年 28 巻 4 号 409-416
    発行日: 1963/12/25
    公開日: 2009/03/19
    ジャーナル フリー
    Electron microscope studies of tips of aerial roots of Chlorophytum capense (L.) Kuntze showed the formation in the nucleus of a large number of invaginations, in which mitchondria accumulate. These mitochondria are seen in many cases attached to the nuclear membrane, which appears destroyed at the point of attachment, as if the mitochondrion were exchanging some substances with the nucleus or were being absorbed by the nucleus. This phenomenon can conceivably be a mechanism to transfer ATP and possibly other substances from the mitochondria to the nucleus.
  • 石野 栞
    日本金属学会会報
    1987年 26 巻 11 号 1044-1046
    発行日: 1987/11/20
    公開日: 2011/08/10
    ジャーナル フリー
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