ラットに

3H

-CU-

83

(S)を25μg/kgで静脈内あるいは経口投与し，血液中濃度および尿糞中排泄を検討した．
静脈内投与後の血液中濃度推移は投与後5分より上昇し，投与後45分に25.74ng eq./mlのC_maxを示し，それ以後t1/

2

3

.05時間とt1/

2

33.09時間の二相で減少した．投与後72時間までのAUCは135.42ng eq.·hr/mlであった．
経口投与では，投与後

3

時間でC_max

4

.10ng eq./mlに達し，以後t1/

2α

4

.46時間とt1/

2β

26.

83

時間の二相で減少した．投与後72時間までのAUCは48.62ng eq.·hr/mlであった．
静脈内投与と経口投与のいずれの場合も，尿および糞中への放射能の排泄は，投与後48時間でほぼ終了した．静脈内投与では，投与後72時間までに投与量の30.52%が尿中に，60.42%が糞中に排泄された．経口投与では，同じく72時間までに40.34%が尿中に，69.24%が糞中に排泄された．

抄録全体を表示

The acetone extracts from three samples of the hepatopancreas of the poisonous scallops obtained on the Okhotsk Coast of Hokkaido Island were fractionated into two parts, hexane soluble fraction (fraction H) and 85% aqueous ethanol soluble fraction (fraction ) by partition to two layers. The majortoxic components in the mouse assay of “diarrheic shellfish toxin” by intra-peritoneal injection were found to be free unsaturated fatty acids showed the following toxicity in MU per g, 18:1 n- 35, 18: n- , 18: n- 167, 18: n- , 20:5 n- 167, and : n- , respectively. Toxicity of the fraction Hin MUper g was much lower than that of the fraction . However, the toxicity of the fraction H per 1 g of the hepatopancreas was about twice that of the fraction , since the fraction Hwas much more abundant than the fraction in the hepatopancreas. The method for the assay of the diarrhetic shellfish toxin must be reexamined by considering the toxic effect of the free unsaturated fatty acids.

抄録全体を表示

The numerical values of the intervals betrween optieal levels are competed for the configurations *⁹4s, 5s, s and 7s of Cu⁺, according to the general expression of energy-levels derived in the previous paper The self-consistent field radial functions computed by Hartree adn Hartree are used for1s, s, p, s, p and . Those of s, 5s, .s and are ealenlated from Hartree Hartree's core-functions by the numerical integrations. The calculated results are shown in Table I.The agreement with experiment is satisfactory

抄録全体を表示

Chemical synthesis of (, 24R)- and (, 24S)-1, 24-dihydroxy--vitamin has been achieved starting with the commercially available dinorcholenic acid acetate. Synthesis involved introduction of the 1-hydroxy group by a reduction of the 1, -epoxide generated by epoxidation of the 1, , -trien--one. The side chain on the steroid was then constructed by means of a Wittig reaction followed by introduction of the Δ⁷ bond by standard methods and its protection with 1-phenyl-1, , -triazoline-, 5-dione. Subsequent reduction of the hydroxy groups in the steroid side chain followed by reduction of the Diels-Alder addition products yielded the both 24-isomers. The 5, 7-dienes were irradiated and the corresponding vitamin compounds isolated. Nuclear magnetic resonance was used to identify individual isomers. The (, 24S)-1, 24-hydroxyvitamin compound bound equally well to the chick intestinal cytosol receptor as 1, 25-dihydroxyvitamin , while the 24R-isomer was approximately ten times less active. In vivo, both isomers were less active than 1, 25-dihydroxyvitamin ; however, the 24S-isomer was considerably more active than the 24R-isomer approaching the activity of 1, 25-dihydroxyvitamin .

抄録全体を表示

性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-AおよびType203で測定した結果,励起光538nm,螢光552.5nmであった。この螢光特性は, およびにそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•5±•5nmを読み,ALLENの補正を行なった。この条件において,および-methyletherの最少検出量は1ngであった。回収率補正の目的で加えた,7-

3H

-

E₂

-17βの全過程における回収率は平均60.

3

±11.7%であった。正常性周期を示す黒毛和種

2

頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共

E₁

,

E₂

の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,

E₂

は

E₁

にくらべ全般に高値であった。また

E₃

は検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.

3

および

99.8ng

/l;

E₁5.9

および16.0ng/l,

E₂29.4

および

83.8ng

/l)を形成し,排卵後は急激に減少して最低値(

3

.

8

~

5.3ng

/l;

E₁1.6

および

1.9ng

/l,

E₂2.2

および

3.4ng

/l)を示した。黄体期の最高値(10.1および27.0ng/l;

E₁2.4

および

3.4ng

/l,

E₂7.7

および

23.6ng

/l)は排卵後

6

~

8

日に認めた。すなわちestrogenの血中濃度は性周期の間に

2

つのピークを形成することを認めた。

抄録全体を表示

A full account is given of the chemical behavior observed for 7, -dialkyladeninium salts (16). On treatment with boiling 1N aqueous NaOH for 60min, 16a, , , (X=I), 16 (X=Br), and 16f (X=) rearranged to isomeric , 7-dialkyladenines (21a-f) in 50-91% yields. Treatment of the salts with 0.5N aqueous at room temperature for 30-90min or with Amberlite CG-400 (OH^-) in at room temperature gave the ring-opened derivatives a-f (in the trans-formamide form) in 56-% yields, and rate constants for the ring-opening reactions of 16a, , -g (X=) and 16 (X=Br) leading to a-g were determined in at pH .84 and ionic strength 0.50 at 25°. Cyclization of a with NaH in at room temperature or with boiling 1N aqueous NaOH produced 21a in 84% or 72% yield, respectively.In solution, the trans-formamides seemed to transform slowly into the cis-formamides 23, attaining equilibria. The existence of such an equilibrium in or - at 25° or in at pH .84 and ionic strength 0.50 at 25° was kinetically confirmed in the case of a, and the mechanism of the rearrangement of 16 to 21 through is discussed on the basis of the above kinetic results and Deslongchamps' theory of stereoelectronic control. On treatment with in MeOH at room temperature, 16a (X=I) furnished the 7, -dihydro derivative 28 (84% yield), which slowly decomposed in at 60° to give a in 49% yield.The 7, -dialkyladeninium salts (16) were found to be obtainable from N'-alkoxy-1-alkyl-5-formamidoimidazole--carboxamidines () through an alternative synthetic route : Alkylations of with alkyl halides in in the absence of base, followed by hydrogenolysis of the N'-alkoxy group and cyclizatio (or vice versa) produced 16 in acceptable yields. In order to interpret the proton nuclear magnetic resonance spectrum of a, the -deuterated species 26 was also synthesized from 24 via 25 and 27.

抄録全体を表示

We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MCT-1. Sixteen phosphate buffered saline (PBS), boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MCT-1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (,24R)-ergosta-7,-diene-,5α,-triol (1). 1 induced ALP activities of MCT-1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MCT-1 cells by 1, ergosterol (), ergocalciferol (), cholesta-,5α,-triol (), 7-dehydrocholesterol (5) and cholecalciferol () were tested. The enzyme activities of MCT-1 cells were increased .0-fold by 10 μM 1 and .-fold by 10 μM . However, , , 5 and did not induce MCT-1 cell ALP activity at 0.1—10 μM. These results suggested that the OH groups at -5 and/or - of 1 and played an important role in their differentiation-inducing activities on MCT-1 cells. Furthermore, 1 suppressed induction of MCT-1 cell apoptosis by serum starvation.

抄録全体を表示

The aim of the present study was to examine the survival rate of embryos after bilateral nonsurgical transfer in recipient cows given a superovulatory treatment of low-dose FSH. In total, 54 parous Holstein or Japanese Black breed cows were used as recipients. These recipients were assigned to the three groups based on hormonal treatments during the preceding estrous cycle. The treatments included A) FSH (10 or 15 mg) and (n=18), ) (n=14), and ) no treatment (n=). Morula to blastocyst-staged embryos that were transferred were derived either from in vitro maturation & in vitro fertilization or they were collected from superovulated Japanese Black heifers. Some embryos collected from donors were routinely frozen and thawed. Most of the cows (%, 15/18) that were given the combination of FSH and formed corpora lutea (CLs) on the ovaries (mean number CLs ± s.. per cow=.1 ± 0.48). Pregnancy rates on day 30 in groups A, and were 67% (12/18), 64% (/14) and 55% (12/), respectively (P>0.05). The rates of twin pregnancies to all pregnancies were 50% (/12), % (/) and 42% (5/12), respectively (P>0.05). Consequently, 19 cows carried their pregnancies to term, producing 25 calves, including six pairs of twins. Calving rates in the three groups were 67% (/12), 33% (/), and 67% (/12), respectively (P>0.05). Calf crop rates were 125% (10/), 133% (/), and 138% (11/), respectively (P>0.05). The embryonic survival rate at Day 30 in group A was likely to be higher than those of cows in the other two groups (50% [18/36] vs 39% [28/72]). No difference between the origins of embryos was observed in the embryonic survival rates. These results suggested that superovulatory treatment with low-dose FSH to recipients bilaterally receiving embryos would be beneficial for increasing embryonic survival, although the results were not statistically significant.

抄録全体を表示

本検討は, 経皮的冠動脈形成術 (PCI) 後の翌日血液透析 (HD) 時における透析中低血圧 (IDH) の発生因子について検討した. 対象は待機的PCI後, 翌日HDを施行した慢性HD患者連続

名 (年齢70±7歳, 男性64名). IDHの定義は, 収縮期血圧20mmHg以上の低下で, 症状を伴いかつHD中断を要したものとした. また, HD記録よりIDHを後方視的に調査し, Logistic回帰分析にてIDHの予測因子を検討した. IDH群 (12名) は非IDH群 (71名) に比して, 有意に低体重 (52.0±. vs. 62.±1.5kg; p=0.007), 造影剤量/体重が多く (.5±0. vs. 1.±0.1mL/kg; p=0.018), 低左室駆出率で (45.±. vs. 56.0±1.% ; p=0.030), /’ が高値 (.±. vs. 17.±0.cm/sec; p=0.038) であった. また, 冠動脈病変や侵襲的な治療について有意差は認めなかった. Logistic回帰分析では, 造影剤量/体重 (OR, .87; 95%CI, 1.-25.; p=0.004) がIDHの有意な予測因子であった. 造影剤使用量が多い症例は, IDHを生じる危険性が高いことが示唆された.

抄録全体を表示

The area investigated is located at longitude 66°30′W and latitude 48°N, on the northern shore of Chaleur Bay. Some 124 specimens (46 samples) were collected from sites in the following geological formations: La Garde (10 samples, late Early to early Middle Devonian), Pirate Cove (11 samples, early Middle Devonian), Fleurant (15 samples, early Late Devonian) and Escuminac (10 samples, early Late Devonian). These formations are composed of conglomerates, silty and calcareous sandstones deposited in a continental environment. The Upper Devonian geological units are amongst a few found in Canada to the east of Alberta. The main goal of this study is a better definition of the Apparent Polar Wander Path of the Appalachians of North America. This study allowed the identification of geological formations favourable to paleomagnetic sampling. Remanent magnetizations and magnetic susceptibilities were measured; both alternating field and thermal demagnetizations were carried out. After demagnetization, components (A, , and ) were isolated and several components were found in each formation. Fold and conglomerate tests were performed; the first is significantly negative except for component and the second is positive in the Fleurant Formation. The mean direction of the components is: 156°, +31° for A, 52°, -46° for , 167°, -21° for and 223°, +25° for . The corresponding paleopoles are °N 141°, °S 77°, 51°N 137° and 17°N °. Based on the fold test, degree of alteration and unblocking temperatures, it was found that components A, and are secondary. Component is either primary or secondary. The relative age of acquisition of magnetization is the following:

B

>

D

>A>. The paleolatitudes vary from 17°S to 10°N going from A to (tilt corrected), to and to . The results are discussed in relation to the Devonian Apparent Polar Wander Path of North America.

抄録全体を表示

The compound, ()-1-(1,-benzodioxol-5-yl)--(-chlorophenyl)prop--en-1-one (I), , crystallizes in the monoclinic crystal system with space group P2₁/ and cell constants: a = .7231(15), = .9239(), = 11.9995(7)Å, β = 99.953()°, V = 1322.37(16), Z = . ()-1-(1,-Benzodioxol-5-yl)--(,-dimethoxyphenyl)prop--en-1-one (II), , crystallizes in the triclinic crystal system, space group P1 with a = .2961(), = 13.8829(), = 14.6713(7)Å, α = 64.185(), β = .560(). γ = 84.966()°, V = 1510.10(10), Z = . Hydrogen bonding and crystal packing effects influence the twist angle between the mean planes of the 1,-benzodioxol-5-yl and benzene groups in both I and II, while π-π stacking interactions help to stabilize the crystal packing.

抄録全体を表示

ウシリンパ球の-ロゼット法の成績に影響する諸要因を検討し, 本法を標準化するため用いる羊血球の品種および個体差, 処理法 (ノイラミニダーゼ(E_N), -アミノエチルイソチオウロニウムブロマイド(E_AET)), ロゼットメディウム, T, 両マーカーをもつリンパ球について検討し, 正常牛リンパ球のサブポピュレーションをしらべた。サフォーク種, コリデール種および雑種の血球間でのロゼット形成率(RFC)には差を認めなかった。処理(E_N, E_AET) 血球のRFCは無処理血球のそれに比べ有意に高かった (P<.01,<.01)。標準法としては, 20%FCS 加 Eagle's MEMリンパ球 (-×10⁶ml)浮遊液とAET処理1%羊赤血球デキストラン (%デキストラン, Mol.wt. 177000)浮 遊液とを等量(400μ1)混合し, 37℃, 30分反応後遠心し, ℃, 16～20時間静置後の算定が適当であった。本法による-ロゼット法, 蛍光標識抗ウシIgGを用いた表面免疫グロブリンの測定およびイースト粒子取込み法による単球の同定法により, 正常牛頭の末梢血単核細胞をTリンパ球 (61.±.%, 56-68%), リンパ球 (.±.1%, 16-30%), 単球 (.1±.%, -10%) に分別し得た。T, 両マーカーをもつリンパ球は約% (0-%) 認められた。

抄録全体を表示

1991年～2005年の福井県など県における散発下痢症患者由来の大腸菌0153107株について, 市販の薬剤感受性ディスクを用いたKB (Kirby-Bauer) 法で12剤の薬剤感受性を調べた. 薬剤別耐性菌出現率はampicillinが72.%, streptomycinが48.%, tetracyclinおよびsulfisoxazoleが46.7%, nalidixic acid (NA) が29.%およびciprofloxacin (CPFX) が24.%などであった. 7～10剤に耐性を示す18株中16株など計26株が, NAおよびCPFXに耐性を示した. NAおよびCPFXに耐性を示した24株とNAに耐性を示した1株について, gyrAおよびparC遺伝子の解析を行った結果, 次のtypesに分けられた. type1 (1株) GyrA (SL) ・ParC (S80I), type (12株) GyrA (SL & 87N) ・ParC (S80I), type (株) GyrA (SL & 87N) ・ParC (S80I & 84G) または (S80R & 84V), type (株) GyrA (SL & 87N) ・ParC (S80I & A108T). アミノ酸変異とfluoroquinolone (FQ) 系薬剤の最小発育阻止濃度 (MIC) との関連をみると, CPFX, ofioxacinおよびnorfloxacinのMICはtype1では, それぞれ1μg/mL, μg/mLおよびμg/mL, typeでは～32μg/mL, ～32μg/mLおよび16～256μg/mL, type, では32～256μg/mL, 32～128μg/mLおよび128～>512μg/mLであった. 患者由来のFQ系剤耐性大腸菌O153が多剤耐性傾向を示すとともに, gyrAおよびparCで各々1～カ所の変異がみられた.

抄録全体を表示

Background: Oxidative stress, the imbalance between pro- and antioxidants, has been implicated in the etiology and pathophysiology of the incidence and mortality of many diseases. We aim to investigate the relations of dietary intakes of vitamin

and and main carotenoids with all-cause mortality in Japanese men and women.

Methods: The Japan Collaborative Cohort Study for Evaluation of Cancer Risk had

,795 men and 35,539 women, aged 40–79 years at baseline (1988–1990), who completed a valid food frequency questionnaire and were followed up to the end of 2009.

Results: There were

,179 deaths in men and 5,355 deaths in women during the median follow-up of 18. years for men and 19. years for women. Multivariate hazard ratios for the highest versus lowest quintile intakes in women were 0. (95% confidence interval [CI], 0.76–0.90; P for trend < 0.0001) for vitamin , 0.85 (95% CI, 0.78–0.93; P for trend < 0.0001) for vitamin , 0. (95% CI, 0.81–0.; P for trend = 0.0006) for β-carotene, and 0.90 (95% CI, 0.82–0.98; P for trend = 0.0002) for β-cryptoxanthin. The joint effect of any two of these highly correlated micronutrients showed significant 12–17% reductions in risk in the high-intake group compared with the low-intake group in women. These significant associations were also observed in the highest quintile intakes of vitamin , vitamin , and β-carotene in female non-smokers but were not observed in female smokers, male smokers, and non-smokers.

Conclusions: Higher dietary intakes of antioxidant vitamins may reduce the risk of all-cause mortality in middle-aged Japanese women, especially female non-smokers.

抄録全体を表示

Mace- extract obtained from seed covering of nutmeg is expected to show an antiarteriosclerotic effect due to its potentiation with unsaturated fatty acid, various vitamins, etc. In this study an effect of Mace- was examined from serum lipid and six constituents of thoracic aorta.
A total of 16 male rabbits of 38 months, including with the Mcce- (M group), with cholesterol ( group) and with Mace- plus cholesterol (MC group) were used. to produce arterosclerosis cholesterol was orally given at 1g to the and MC group. To the M and MC groups Mace- was given at 500mg/day. Sixteen weeks later referring to all the rabbits, total cholesterol (T. CHO), triglyceride (TRIG), β-lipoprotein (β-LIPO), HDL-cholesterol (HDL), in total of serum lipids, were measured. Furthermore, all the rabbits were killed and constituents media of thoracic aorta were stained and observed histochemically, i. ., SMC with Azocarmine G stain, EL with Weigert's stain, CL with Van Gieson's stain, AMPS with Alcian blue stain and GP with PAS stain. T. CHO was 90.5mg/dl in M group, 369.mg/dl in group and 81.mg/dl in MC group respectively. The MC group showed no difference with M group but the significantly lower value than that of group (p<0.01). TRIG was 108.5mg/dl in M group, .mg/dl in group and 94.mg/dl in MC group respectively. No significant difference was observed among these groups. Beta-LIPO was 184.mg/dl in M group, 995.mg/dl in group and 151.5mg/dl in MC group respectively. Compared with group, M and MC groups showed significantly low value (p<0.001-p<0.01). HDL was 18.mg/dl in M group, .mg/dl in group and 26.5mg/dl in MC group respectively. There was no significant difference among those group, although it was highest in MC group. When histochemically observed, group was strongly disturbed, while MC group showed slight swelling of SMC and cell nucleus that was not remarkable though, and damage of EL and CL was minimal. AMPS and GP remained unchanged. From changes of serum lipid and observation of arterial tissue, it was suggested that Mace- has an antisclerotic effect.

抄録全体を表示