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  • 大澤 康次, 幡野 恵, 西宮 一尋, 岡崎 彬, 太田 真一, 宇田 文昭, 柳田 由紀, 檜垣 洋子, 吉田 知江里
    薬物動態
    1988年 3 巻 4 号 441-446
    発行日: 1988年
    公開日: 2007/03/29
    ジャーナル フリー
    ラットに
    3H
    -CU-
    83
    (S)を25μg/kgで静脈内あるいは経口投与し,血液中濃度および尿糞中排泄を検討した.
    静脈内投与後の血液中濃度推移は投与後5分より上昇し,投与後45分に25.74ng eq./mlのCmaxを示し,それ以後t1/2
    3
    .05時間とt1/2 33.09時間の二相で減少した.投与後72時間までのAUCは135.42ng eq.·hr/mlであった.
    経口投与では,投与後
    3
    時間でCmax 4.10ng eq./mlに達し,以後t1/2α 4.46時間とt1/2β 26.
    83
    時間の二相で減少した.投与後72時間までのAUCは48.62ng eq.·hr/mlであった.
    静脈内投与と経口投与のいずれの場合も,尿および糞中への放射能の排泄は,投与後48時間でほぼ終了した.静脈内投与では,投与後72時間までに投与量の30.52%が尿中に,60.42%が糞中に排泄された.経口投与では,同じく72時間までに40.34%が尿中に,69.24%が糞中に排泄された.
  • Yosizo YAMAMOTO
    Nippon Sugaku-Buturigakkwai Kizi Dai
    3
    Ki

    1940年 22 巻 12 号 1048-1052
    発行日: 1940年
    公開日: 2009/06/09
    ジャーナル フリー
    The numerical values of the intervals betrween optieal levels are competed for the configurations
    1s22s22p83s23p83
    *94s, 5s, 6s and
    7
    s of Cu+, according to the general expression of energy-levels derived in the previous paper The self-consistent field radial functions computed by Hartree adn Hartree are used for1s, 2s, 2p,
    3
    s,
    3
    p and
    3
    d
    . Those of 4s, 5s,
    e
    .s and are ealenlated from Hartree Hartree's core-functions by the numerical integrations. The calculated results are shown in Table I.The agreement with experiment is satisfactory
  • Keishi Hata, Fuyuki Sugawara, Naganori Ohisa, Saori Takahashi, Kazuyuki Hori
    Biological and Pharmaceutical Bulletin
    2002年 25 巻 8 号 1040-1044
    発行日: 2002年
    公開日: 2002/08/01
    ジャーナル フリー
    We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC
    3
    T
    3
    -
    E
    1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC
    3
    T
    3
    -
    E
    1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (
    22E
    ,24R)-ergosta-
    7
    ,
    22
    -diene-
    ,5α,6β-triol (1). 1 induced ALP activities of MC
    3
    T
    3
    -
    E
    1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC
    3
    T
    3
    -
    E
    1 cells by 1, ergosterol (2), ergocalciferol (
    3
    ), cholesta-
    ,5α,6β-triol (4),
    7
    -dehydrocholesterol (5) and cholecalciferol (6) were tested. The enzyme activities of MC
    3
    T
    3
    -
    E
    1 cells were increased
    3
    .0-fold by 10 μM 1 and 2.4-fold by 10 μM 4. However, 2,
    3
    , 5 and 6 did not induce MC
    3
    T
    3
    -
    E
    1 cell ALP activity at 0.1—10 μM. These results suggested that the OH groups at C-5 and/or C-6 of 1 and 4 played an important role in their differentiation-inducing activities on MC
    3
    T
    3
    -
    E
    1 cells. Furthermore, 1 suppressed induction of MC
    3
    T
    3
    -
    E
    1 cell apoptosis by serum starvation.
  • 浅岡 顕, 小高 猛司
    土質工学会論文報告集
    1992年 32 巻 3 号 117-129
    発行日: 1992/09/15
    公開日: 2008/02/29
    ジャーナル フリー
    Failure problems of saturated soils are classified in the present study into the following four types : Type I : The failure of loose and/or normally consolidated soils under fully drained loading conditions. Type II : The same soils but under perfectly undrained conditions. Type III : The failure of dense and/or overconsolidated soils under fully drained conditions. Type IV : The same soils but under perfectly undrained conditions. The soil-water coupling limiting equilibrium analysis on the basis of the critical state concept is shown possible to draw a distinction between type I, II and IV problems, and the limitations of the analysis procedure happen in solving the type problem in the above. This is demonstrated experimentally, in which seepage failure experiments in laboratory with the use of a saturated silty sand are employed to make distinctions clearly between the four types of failure problems. The definite distinctions both in failure load and failure mode appeared in the four types of experiments are summarized as follows : (1) the smallest failure load in loose sand in the undrained condition, (2) the largest failure load with the largest failure region in dense sand under undrained loading, (
    3
    ) the observation of no global deformation before failure within the soil under the fully drained condition that suggests the development of very localized shear deformation in the sand. Three out of four experiments are described well by the limit analysis computation mentioned above as far as the failure load and the shape and the size of the failure region after boiling failure are concerned, and thus the type III problem is identified to be the problem that should be solved by any other means in near future.
  • 高木 徹, 林 賢治, 板橋 豊
    日本水産学会誌
    1984年 50 巻 8 号 1413-1418
    発行日: 1984/08/25
    公開日: 2008/02/29
    ジャーナル フリー
    The acetone extracts from three samples of the hepatopancreas of the poisonous scallops obtained on the Okhotsk Coast of Hokkaido Island were fractionated into two parts, hexane soluble fraction (fraction H) and 85% aqueous ethanol soluble fraction (fraction
    E
    ) by partition to two layers. The majortoxic components in the mouse assay of “diarrheic shellfish toxin” by intra-peritoneal injection were found to be free unsaturated fatty acids showed the following toxicity in MU per g, 18:1 n-
    9
    35, 18:2 n-6
    83
    , 18:
    3
    n-
    3
    167, 18:4 n-
    3
    83
    , 20:5 n-
    3
    167, and
    22
    :6 n-
    3
    83
    , respectively. Toxicity of the fraction Hin MUper g was much lower than that of the fraction
    E
    . However, the toxicity of the fraction H per 1 g of the hepatopancreas was about twice that of the fraction
    E
    , since the fraction Hwas much more abundant than the fraction
    E
    in the hepatopancreas. The method for the assay of the diarrhetic shellfish toxin must be reexamined by considering the toxic effect of the free unsaturated fatty acids.
  • 斉 洋之, 高津戸 秀, 池川 信夫, 田中 洋子, スミス コニー, デルカ F.ヘクター
    Chemical and Pharmaceutical Bulletin
    1984年 32 巻 10 号 3866-3872
    発行日: 1984/10/25
    公開日: 2008/03/31
    ジャーナル フリー
    Chemical synthesis of (
    22
    E
    , 24R)- and (
    22
    E
    , 24S)-1, 24-dihydroxy-
    Δ22
    -vitamin
    D3
    has been achieved starting with the commercially available dinorcholenic acid acetate. Synthesis involved introduction of the 1-hydroxy group by a reduction of the 1, 2-epoxide generated by epoxidation of the 1, 4, 6-trien-
    3
    -one. The side chain on the steroid was then constructed by means of a Wittig reaction followed by introduction of the
    Δ7
    bond by standard methods and its protection with 1-phenyl-1, 2, 4-triazoline-
    3
    , 5-dione. Subsequent reduction of the hydroxy groups in the steroid side chain followed by reduction of the Diels-Alder addition products yielded the both 24-isomers. The 5,
    7
    -dienes were irradiated and the corresponding vitamin
    D
    compounds isolated. Nuclear magnetic resonance was used to identify individual isomers. The (
    22
    E
    , 24S)-1, 24-hydroxyvitamin
    D3
    compound bound equally well to the chick intestinal cytosol receptor as 1, 25-dihydroxyvitamin
    D3
    , while the 24R-isomer was approximately ten times less active. In vivo, both isomers were less active than 1, 25-dihydroxyvitamin
    D3
    ; however, the 24S-isomer was considerably more active than the 24R-isomer approaching the activity of 1, 25-dihydroxyvitamin
    D3
    .
  • Hiroshi Xavier Chiura, Masataka Umitsu
    Microbes and Environments
    2004年 19 巻 1 号 20-30
    発行日: 2004年
    公開日: 2004/04/21
    ジャーナル フリー
    Virus-like particles (VLPs) were collected from geothermal vent water samples in the drift-way at Toyoha Mine, Hokkaido, Japan (−500 m level, 63.5°C) whose VLP and bacterial abundance was (No/ml±SD, n: 500), VLP:
    9
    .60±0.29×108 and bacteria:
    3
    .61±0.14×106. VLPs ranged in diameter from 30 to 320 nm, and the major size distribution (ca 62%) was
    83
    .33.
    3
    nm (n: 843). Ultrafiltration followed by CsCl density equilibrium ultracentrifugation gave purified TY-VLPs: 6.64×1013. Regardless of UV treatment, TY-VLP reduced the efficiency of plating to 68.6-
    83
    .4% at a multiplicity of infection of ca 0.
    3
    on Escherichia coli
    AB
    1157. Generalised transduction was observed on
    E
    . coli
    AB
    1157 with a frequency between 10-4 and 10-5 cells/particle using TY-VLPs without UV-treatment. The growth of generated
    E
    . coli
    transductants (TY-
    E
    -trans) was compared to that of an
    E
    . coli
    transductant (ST-
    E
    -trans) generated by Aquificales originating VLP (Chiura, 2002). The extent of the maximum growth of both transductants was ca 40% of the parental
    E
    . coli
    used as a recipient. TY-
    E
    -trans acquired "budding-like" particle productivity, which has been demonstrated for ST-
    E
    -trans. ST-
    E
    -trans produced five different size particles, whose DNA content ranged between 291.6 and 382.0 kb, and TY-
    E
    -trans produced ten different size particles between 68.5 and 190.2 kb, respectively.
  • 石畝 史, 東方 美保, 山崎 貢, 松雪 星子, 森屋 一雄, 田中 大祐, 磯部 順子, 京田 芳人, 村岡 道夫
    感染症学雑誌
    2006年 80 巻 5 号 507-512
    発行日: 2006/09/20
    公開日: 2011/02/07
    ジャーナル フリー
    1991年~2005年の福井県など4県における散発下痢症患者由来の大腸菌0153107株について, 市販の薬剤感受性ディスクを用いたKB (Kirby-Bauer) 法で12剤の薬剤感受性を調べた. 薬剤別耐性菌出現率はampicillinが72.
    9
    %, streptomycinが48.6%, tetracyclinおよびsulfisoxazoleが46.
    7
    %, nalidixic acid (NA) が29.
    9
    %およびciprofloxacin (CPFX) が24.
    3
    %などであった.
    7
    ~10剤に耐性を示す18株中16株など計26株が, NAおよびCPFXに耐性を示した. NAおよびCPFXに耐性を示した24株とNAに耐性を示した1株について, gyrAおよびparC遺伝子の解析を行った結果, 次の4typesに分けられた. type1 (1株) GyrA (S
    83
    L) ・ParC (S80I), type2 (12株) GyrA (S
    83
    L &
    D
    87N) ・ParC (S80I), type
    3
    (8株) GyrA (S
    83
    L &
    D
    87N) ・ParC (S80I &
    E
    84G) または (S80R &
    E
    84V), type4 (4株) GyrA (S
    83
    L &
    D
    87N) ・ParC (S80I & A108T). アミノ酸変異とfluoroquinolone (FQ) 系薬剤の最小発育阻止濃度 (MIC) との関連をみると, CPFX, ofioxacinおよびnorfloxacinのMICはtype1では, それぞれ1μg/mL, 2μg/mLおよび8μg/mL, type2では8~32μg/mL, 8~32μg/mLおよび16~256μg/mL, type
    3
    , 4では32~256μg/mL, 32~128μg/mLおよび128~>512μg/mLであった. 患者由来のFQ系剤耐性大腸菌O153が多剤耐性傾向を示すとともに, gyrAおよびparCで各々1~2カ所の変異がみられた.
  • PIETRO DE RUGGIERI, Carmelo Gandolfi, Umberto Guzzi
    International Symposium on the Chemistry of Natural Products
    1964年 1964 巻 E-7-3
    発行日: 1964/04/12
    公開日: 2017/08/18
    会議録・要旨集 フリー
  • 大原 信行
    日本消化器がん検診学会雑誌
    2017年 55 巻 6 号 1045-1052
    発行日: 2017年
    公開日: 2017/12/13
    ジャーナル フリー
    2014年LIA法を原理とする血清H. pylori抗体価測定キット「LZテスト」が上市された。LZテストは従来のELISA法による
    E
    プレートとは異なる測定系であり,
    E
    プレートに関する知見が通用する保証はない。今回, LZテストの陰性高値(抗体価
    3
    .0~
    9
    .
    9
    U/ml)が
    E
    プレートと同様の取扱いができるかを検証した。対象は両検査法で抗体価を同時測定し両抗体価の少なくとも一方が10U/ml未満であった230例であり, 陰性高値内のH. pylori感染状態の構成比および“新ABC分類”(カットオフ値=
    3
    .0U/ml)による分類結果を比較した。
    E
    プレートでは陰性高値となる未感染は存在せず, LZテストでは16例(
    22
    .5%)であった。未感染98例に新ABC分類を適応すると
    E
    プレートではA群97例(99.0%)
    D
    群1例(1.0%), LZテストではA群
    83
    例(84.
    7
    %), BCD群合わせて15例(15.
    3
    %)であった。LZテストの陰性高値には未感染が多く含まれ, 新ABC分類をLZテストで行うと未感染者を要精検とする割合が増えるため, 未感染者が多数を占める検診では
    E
    プレートを用いることが望ましい。
  • 馬場 輝郎
    照明学会雑誌
    1968年 52 巻 3 号 107-111
    発行日: 1968/03/25
    公開日: 2011/07/19
    ジャーナル フリー
  • 百目鬼 郁男, 中原 達夫, 山内 亮
    家畜繁殖研究會誌
    1974年 20 巻 2 号 76-80
    発行日: 1974/08/30
    公開日: 2008/05/15
    ジャーナル フリー
    性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
    Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-2AおよびType203で測定した結果,励起光538nm,螢光552.5nmであった。この螢光特性は
    E1
    ,
    E2
    および
    E3
    にそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•5±
    22
    •5nmを読み,ALLENの補正を行なった。この条件において
    E1
    ,
    E2
    および
    E3
    -methyletherの最少検出量は1ngであった。回収率補正の目的で加えた6,
    7
    -
    3H
    -
    E2
    -17βの全過程における回収率は平均60.
    3
    ±11.
    7
    %であった。正常性周期を示す黒毛和種2頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共
    E1
    ,
    E2
    の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,
    E2
    E1
    にくらべ全般に高値であった。また
    E3
    は検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.
    3
    および99.8ng/l;
    E15.9
    および16.0ng/l,
    E229.4
    および
    83.8ng
    /l)を形成し,排卵後は急激に減少して最低値(
    3
    .8~
    5.3ng
    /l;
    E11.6
    および
    1.9ng
    /l,
    E22.2
    および
    3.4ng
    /l)を示した。黄体期の最高値(10.1および27.0ng/l;
    E12.4
    および
    3.4ng
    /l,
    E27.7
    および23.6ng/l)は排卵後6~8日に認めた。すなわちestrogenの血中濃度は性周期の間に2つのピークを形成することを認めた。
  • 膵臓
    2007年 22 巻 1 号 e350-e352
    発行日: 2007年
    公開日: 2007/03/27
    ジャーナル フリー
  • II動物の健康時における血液ガス値について
    小笠原 成郎
    日本獣医師会雑誌
    1960年 13 巻 10 号 434-438
    発行日: 1960/10/20
    公開日: 2011/06/17
    ジャーナル フリー
    The following average values were determined. on all or some of 27 Saanen goats, 19 Holstein-Friesian and 4 Jersey cows,
    7
    Japanese native black oxen, and 13 dogs, all of which were healthy.
    1. The oxygen content on a volume per cent basis of the arterial blood (
    AB
    ) was 14.059 in goats (G) and 14.71 in cattle (C). That of the venous blood (VB) was
    9
    .72 in G, 10.71 in C, and 10.74 in dogs (
    D
    ).
    2. The oxygen capacity on a volume per cent basis was 15.25 in G, 15.98 in C, and 16.66 in
    D
    .
    3
    . Oxygen per cent saturation in
    AB
    was
    89
    .37 in G and 91.5 in C. That in VB was 63.
    83
    in G, 67.20 in C, and 64.71 in
    D
    .
    4. The CO2 pressure in mmHg of
    AB
    was 41. 76 in G and 37.
    9
    in C. That of VB was 47.08 in G, 42.6 in C, and 43.5 in
    D
    .
    5. The total CO2 on a volume per cent (and MM/L) basis of
    AB
    was 56.43 (25.63) in G and 52.08 (23.55) in C. That of VB was 61.08 (27.63) in G, 56.53 (25.53) in C, and 58.44 (26.40) in
    D
    .
    6. The free CO2 (as
    H2CO3
    ) on an MM/L basis of
    AB
    was 1.31 in G and 1.19 in C. That of VB was 1.47 in G, 1.33 in C, and 1.36 in
    D
    .
    7
    . The combinative CO2 (as
    HCO3
    -) on an ME Q/L basis of
    AB
    was 25.12 in G and
    22
    .61 in C. That of VB was 26.10 in G, 24.19 in C, and 25. 12 in
    D
    .
    8. The plasma buffer base (BB+) on an mEQ/L basis of
    AB
    was 42.57 in G and 40.2 in C. That of VB was 43.46 in G, 41.
    3
    in C, and 41.98 in
    D
    .
    9
    . The pH of
    AB
    was
    7
    .39 in G and
    7
    .40 in C. That of VB was
    7
    .36 in G and C and
    7
    .37 in
    D
    .
  • Manabu NUKINA, Shin ITO, Takayuki KUREBAYASHI
    Bioscience, Biotechnology, and Biochemistry
    1996年 60 巻 12 号 2097-2098
    発行日: 1996/12/23
    公開日: 2008/02/08
    ジャーナル フリー
    Indene was found to be microbially transformed to (-)-(1R, 2S)-cis-1, 2-indandiol (
    7
    .
    3
    % yield, 15%
    e.e
    .), (+)-(1R, 2R)-trans-1, 2-indandiol (
    3
    %) yield,
    83
    %
    e.e
    .), (-)-(R)-indenol (0.1% yield,
    89
    %
    e.e
    .), (+)-(S)-2-hydroxy-1-indanone (0.1% yield, 31%
    e.e
    .), 1-indanone (0.2% yield), and (+)-(S)-1-indanol (trace, 99%
    e.e
    .) by the Pyricularia zingiberi fungus.
  • / 石原 研而, KENJI ISHIHARA
    地盤工学会論文報告集
    2000年 40 巻 4 号 103-119
    発行日: 2000/08/15
    公開日: 2008/02/29
    ジャーナル フリー
    The flow deformation or strain softening in undrained shear of saturated sand is discussed in this paper. When evaluating the flow of field deposits, the prime issue to be addressed is whether the soil in its in-situ state has the potential to develop flow deformation or not. This paper presents a rational method which allows for such an assessment to be made and aims at quantifying the effects of grain-size distribution on the flow potential of sandy soils. On the basis of existing laboratory and field test results on sandy soils, a flow potential formulation was developed within the framework of the state concept. Here, the SPT blow count was used as a parameter for field characterization of sand deposits whereas the void ratio range (emax-emin) was employed as a measure indicative of the grading properties of sandy soils. It is shown that sands with a large value of void ratio range have a high flow potential, indicating that fines-containing sands are more susceptible to flow than clean sands. Results of this study also suggest that flow with zero residual strength is limited to field deposits with a very low SPT blow count, and that soils with a void ratio range of less than 0.35, such as coarse sands and gravels, are practically safe against flow with zero residual strength.
  • 斉 洋之, 高津戸 秀, 池川 信夫
    Chemical and Pharmaceutical Bulletin
    1985年 33 巻 11 号 4815-4820
    発行日: 1985/11/25
    公開日: 2008/03/31
    ジャーナル フリー
    Two new vitamin
    D3
    analogues, 1α-hydroxy-24, 24-dimethyl-
    22
    E
    -dehydrovitamin
    D3
    (13) and 1α, 25-dihydroxy-24, 24-dimethyl-
    22
    E
    -dehydrovitamin
    D3
    (17), which are blocked for 24-hydroxylation by the methyl groups, were synthesized from 1α,
    -bismethoxymethoxypregn-5-ene-20Scarbaldehyde (6) by using the orthoester Claisen rearrangement for construction of the carbon skeleton of their side chains. These compounds (13 and 17) elicited a rise in serum calcium, but not in serum inorganic phosphorus in rats. In a bioassay for alkaline phosphatase, they were found to show much weaker activity than 1α-hydroxy vitamin
    D3
    (5).
  • 高次脳機能研究 (旧 失語症研究)
    2019年 39 巻 1 号 55-56
    発行日: 2019/03/31
    公開日: 2020/04/03
    ジャーナル フリー
  • Wei Xu, Rui Xia, Feng Tian, Lei Liu, Meng Li, Shiyuan Fang
    Journal of Hard Tissue Biology
    2022年 31 巻 4 号 263-268
    発行日: 2022年
    公開日: 2022/10/27
    ジャーナル フリー

    Fracture healing is a complex dynamic process that involves the balance between osteoblasts and osteoclasts. Several microRNAs (miRNAs) have been shown to participate in fracture healing. In this study, we investigated the role of miR-324-

    3
    p in osteoblast differentiation. MC
    3
    T
    3
    -
    E
    1 cell differentiation was induced by icariin, and miR-324-
    3
    p expression levels during cell differentiation were measured using qRT-PCR. Cell proliferation and differentiation were assessed to evaluate the function of miR-324-
    3
    p. Luciferase activity was used for target gene verification. During MC
    3
    T
    3
    -
    E
    1 differentiation, miR-324-
    3
    p levels gradually increased over time. Further experiments showed that miR-324-
    3
    p overexpression significantly promoted cell viability, whereas miR-324-
    3
    p downregulation showed the opposite effect. For cells with miR-324-
    3
    p mimic, the levels of bone sialoprotein, Runx2, osteocalcin, and alkaline phosphatase activity were significantly elevated. SMAD
    7
    is the target gene of miR-324-
    3
    p, and its level is gradually downregulated during MC
    3
    T
    3
    -
    E
    1 cell differentiation. MiR-324-
    3
    p may promote MC
    3
    T
    3
    -
    E
    1 cell differentiation by targeting SMAD
    7
    .

  • 村上 孝夫, 木村 毅, 和田 浩志, 田中 信寿, 斎木 保久, 陳 秋明
    Chemical and Pharmaceutical Bulletin
    1981年 29 巻 3 号 866-868
    発行日: 1981/03/25
    公開日: 2008/03/31
    ジャーナル フリー
    From the fronds of Polystichum tripteron (KUNZE) PR. a new norcarotenoid glycoside was isolated and shown to be (6R,
    7
    E
    ,
    9
    R)-
    9
    -hydroxy-megastigma-4,
    7
    -dien-
    3
    -one-
    9
    -O-β-
    D
    -glucoside. The fronds of Dennstaedtia wilfordii (MOORE) CHRIST. contain the same glucoside.
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